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Research Article

接触石棉样纤维的肺和淋巴结组织中MacroH2A.1的体内免疫组化研究

卷 20, 期 8, 2020

页: [653 - 660] 页: 8

弟呕挨: 10.2174/1566524020666200220130023

价格: $65

摘要

目标：本研究的目的是研究MacroH2A.1 在FE暴露绵羊组织中的免疫表达。 背景：石棉纤维、肺癌、胸膜间皮瘤和其他肺部疾病之间的相关性已经被确定为吸入氟齿矿(FE)所涉及的病理生理呼吸机制。后者表现为细胞增殖，诱导生长因子、细胞因子、活性氧和亚硝酸盐的释放，导致DNA损伤，导致慢性炎症和癌变。MacroH2A.1和组蛋白变体，似乎在感知细胞代谢状态和连接它与染色质的作用。生理学上，MacroH2A.1在干细胞中低水平表达，并在分化过程中上调，防止诱导多能干细胞的重编程和核移植后。特别是,MacroH2A.1已被证明在体内阐明了一种有效的抗肿瘤机制，因为它在衰老细胞中上调，从而决定永久性的生长停滞。 目的：评估组蛋白变体在机体中可能的作用，以深入了解毒性机制和细胞对铁的反应。 方法：选择暴露绵羊的肺和淋巴结。对样品进行组织学和免疫组化检测。进行了密度测定、形态测定和统计分析分析。 结果：与未暴露的样品相比，暴露铁的绵羊组织切片显示MacroH2A.1过表达。数据表明，这些分子参与了由铁直接暴露引发的细胞反应。 结论：在本次竞赛中，MacroH2A.1过表达支持其作为表观遗传稳定剂的功能，帮助建立和维持分化状态。

关键词: 肺，石棉，癌症，肿瘤，H2AFY，核心组蛋白宏- H2A.1，代谢。

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