Generic placeholder image

Current Nutrition & Food Science

Editor-in-Chief

ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

Research Article

Sauerkraut with the Addition of Lactobacillus casei: Effects of Salt and Sugar Concentrations on Fermentation and Antioxidant Activity

Author(s): Elok Zubaidah*, Mentari S. Arum, Tri D. Widyaningsih and Aldila P. Rahayu

Volume 16, Issue 8, 2020

Page: [1265 - 1269] Pages: 5

DOI: 10.2174/1573401316666200217112642

Abstract

Background: Spontaneous fermentations have several disadvantages, and additions of lactic acid bacteria can improve sauerkraut fermentations and quality. Salt and sugar concentrations are important factors in sauerkraut fermentations, and the growth and activity of lactic acid bacteria can affect antioxidant activity of sauerkrauts.

Objective: This study developed sauerkrauts with the addition of Lactobacillus casei and investigated how salt and sugar concentrations affected their fermentation and antioxidant activity.

Methods: A fresh cabbage was washed and cut, before salt (1.0%; 1.5% and 2.0%) and sugar (0% and 2.0%) were added, prior to inoculation with a L. casei culture at 10% (v/w). The cabbage was then incubated at 28oC for 5 days. The controlled lactic fermentation of the cabbage without culture and sugar, but with salt at 2.5% was performed. The sauerkrauts were evaluated for total lactic acid bacteria, pH, total acidity, phenolic content and DPPH scavenging activity.

Results: The fermentation increased the total lactic acid bacteria counts in the sauerkrauts from 1.38 x 109 to 3.86 x 109 cfu/mL, and this demonstrated significant (p<0.05) direct salt and sugar effects. The high salt and sugar concentrations also increased the total acidity, antioxidant activity and phenolic content, but lowered the pH of the sauerkrauts. The additives (L. casei culture, salt and sugar), therefore, improved the sauerkraut fermentation and antioxidant activity compared to the control.

Conclusion: With added sugar and salt, L. casei improved the sauerkraut fermentation and antioxidant activity, and both sugar and salt can each be added at a low (2%) concentration for maximum effects.

Keywords: Antioxidant activity, cabbage, fermentation, Lactobacillus casei, salt, sauerkraut, sugar.

Graphical Abstract

[1]
Farnworth ER, Mainville I. Kefir: A fermented milk product. Farnworth ER, Ed Handbook of fermented functional foods, 1st ed BR: CRC Press. 2003; pp. 79-115.
[2]
Trail AC, Fleming HP, Young CT, McFeeters RF. Chemical and sensory characterization of commercial sauerkraut. J Food Qual 1996; 19: 15-30.
[http://dx.doi.org/10.1111/j.1745-4557.1996.tb00402.x]
[3]
Lu Z, Breidt F, Plengvidhya V, Fleming HP. Bacteriophage ecology in commercial sauerkraut fermentations. Appl Environ Microbiol 2003; 69(6): 3192-202.
[http://dx.doi.org/10.1128/AEM.69.6.3192-3202.2003] [PMID: 12788716]
[4]
Plengvidhya V, Breidt F Jr, Lu Z, Fleming HP. DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations. Appl Environ Microbiol 2007; 73(23): 7697-702.
[http://dx.doi.org/10.1128/AEM.01342-07 PMID: 17921264]
[5]
Ciska E, Magdalena K, Agnieszka K. Antioxidant activity of extracts of white cabbage and sauerkraut. Pol J Food Nutr Sci 2005; 14/55(4): 367-73.
[6]
Podsędek A. Natural antioxidants and antioxidant capacity of Brassica vegetables: a review. Lebensm Wiss Technol 2007; 40: 1-11.
[http://dx.doi.org/10.1016/j.lwt.2005.07.023]
[7]
Penas E, Frias J, Sidro B, Vidal-Valvede C. Chemical evaluation and sensory quality of sauerkraut obtained fermentations at different NaCl levels from Brassica oleracea Var. capitata Cv. Bronco grown in eastrent Spain. Effect of storage. J Agric Food Chem 2010; 58: 3549-57.
[http://dx.doi.org/10.1021/jf903739a PMID: 20170112]
[8]
Karimi R, Mortazavian AM, Karami M. Incorporation of Lactobacillus casei in Iranian ultrafiltered Feta cheese made by partial replacement of NaCl with KCl. J Dairy Sci 2012; 95(8): 4209-22.
[http://dx.doi.org/10.3168/jds.2011-4872 PMID: 22818434]
[9]
Marinaki E, Kandylis P, Dimitrellou D, Zakynthinos G, Varzakas T. Probiotic Yogurt production with Lactobacillus casei and prebiotics Curr Res Nutr Food Sci 2016; 4(Sl. 2): 48-53.
[10]
Qu L, Ren J, Huang L, et al. Anti-diabetic effects of Lactobacillus casei fermented-yogurt through re-shaping gut microbiota structure in type 2 diabetic rats. J Agric Food Chem 2018; 66(48): 12696-705.
[http://dx.doi.org/10.1021/acs.jafc.8b04874 PMID: 30398060]
[11]
Bellinazo PL, Vitola HRS, Cruxen CES, Braun CLK, Hackbart HCS, da Silva WP, et al. Viability of Lactobacillus casei strains and bixin antioxidant effect (Bixa orellana L.). J Food Process Preserv 2019; 43(9)e14088
[http://dx.doi.org/10.1111/jfpp.14088]
[12]
Du R, Song G, Zhao D, Sun J, Ping W, Ge J. Lactobacillus casei starter culture improves vitamin content, increases acidity and decreases nitrite concentration during sauerkraut fermentation. Int J Food Sci Technol 2018; 53(8): 1925-31.
[http://dx.doi.org/10.1111/ijfs.13779]
[13]
Xiong T, Li J, Liang F, Wang Y, Guang Q, Xie M. Effects of salt concentration on Chinese sauerkraut fermentation. Lebensm Wiss Technol 2016; 69: 169-74.
[http://dx.doi.org/10.1016/j.lwt.2015.12.057]
[14]
Penas E, Martinez-Villaluenga C, Frias J, Jose M. Se improves indole glucosinolate hydrolysis product content, Se-Methylselenocysteine content, antioxidant capacity and potential anti-inflammatory properties of sauerkraut. Food Chem 2012; 132: 907-14.
[http://dx.doi.org/10.1016/j.foodchem.2011.11.064]
[15]
Zubaidah E, Dewantari FJ, Novitasari FR, Srianta I, Blanc PJ. Potential of snake fruit (Salacca zalacca (Gaerth.) Voss) for the development of a beverage through fermentation with the Kombucha consortium. Biocatal Agric Biotechnol 2018; 13: 198-203.
[http://dx.doi.org/10.1016/j.bcab.2017.12.012]
[16]
Yang J, Paulino R, Janke-Stedronsky S, Abawi F. Free radical scavenging activity and total phenol of noni (Moriada eitrifolia L.) juice and powder in processing and storage. Food Chem 2007; 102(1): 302-8.
[http://dx.doi.org/10.1016/j.foodchem.2006.05.020]
[17]
Molyneux P. The use of the stable free radical diphenyl picrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004; 26(2): 211-9.
[18]
Steinkraus KH. Fermentations in world food processing. Compr Rev Food Sci Food Saf 2002; 1: 23-32.
[http://dx.doi.org/10.1111/j.1541-4337.2002.tb00004.x]
[19]
Marianski S. The art of making fermented sausages. USA: Bookmagic LLC 2008.
[20]
Goh WN, Rosma A, Kaur B, Fazilah A, Karim AA, Bhat R. Fermentation of black tea broth (Kombucha): I. effects of sucrose concentration and fermentation time on the yield of microbial cellulose. Int Food Res J 2012; 19(1): 109-17.
[21]
Essawet HA, Cvetkovic D, Velincanski A, et al. Polyphenol and antioxidant activities of kombucha beverage enriched with coffeeberry extract. Chem Ind Chem Eng Q 2015; 21(3): 399-409.
[http://dx.doi.org/10.2298/CICEQ140528042E]
[22]
Martinez C, Penas E, Sidro B, Ullate M, Frias J, Vidal V. White cabbage fermentation improves ascorbate content, antioxidant and nitric oxide production inhibitory activity in LPS-induced macrophages. Lebensm Wiss Technol 2012; 46(1): 77-83.
[http://dx.doi.org/10.1016/j.lwt.2011.10.023]
[23]
Dipti P, Yogesh B, Kain AK, et al. Lead induced oxidative stress: beneficial effects of kombucha tea. Biomed Environ Sci 2003; 16(3): 276-82.
[PMID: 14631833]
[24]
Badarinath A, Rao K, Chetty CS, Ramkanth S, Rajan T, Gnanaprakash KA. Review on in-vitro antioxidant methods: comparisons, correlations, and considerations. Int J Pharm Tech Res 2010; 2(2): 1276-85.

© 2024 Bentham Science Publishers | Privacy Policy