Login Register Cart 0
Generic placeholder image

Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Ointment Formulation and Characterization of Arum Manis Mango (Mangifera indica L.) Leaves’ Methanol Extract as an Antibacterial Agent Against Propionibacterium acnes

Author(s): Dian R. Ningsih*, Zusfahair Zusfahair , Dadan Hermawan, Wulan Anggraeni and Hassan Y. Aboul-Enein*

Volume 17, Issue 1, 2021

Published on: 14 February, 2020

Page: [67 - 75] Pages: 9

DOI: 10.2174/1573407216666200214100855

Price: $65

Abstract

Background: Acne is caused by several factors, including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One plant which has the potential as an antibacterial agent is the extract of Arum Manis mango leaves.

Methods: The Minimum Inhibitory Concentration (MIC) of methanol extract of mango leaves was determined, which can inhibit Propionibacterium acnes activity. The antibacterial activity tests were performed using agar diffusion. The ointment formulation, the characteristics of ointment preparations, and the ointment activity against P. Acnes are discussed.

Results: MIC of methanol extract of mango leaves value is 5 ppm with an inhibition zone of 1 mm. The ointment obtained is white, has a distinctive smell, semisolid form, possesses a pH of 4.92 - 5.87, dispersive power of 5.05 - 6.30 cm, and adhesive power of 1 - 3.67 seconds, homogeneous and protective. Ointment preparations of methanol extract of mango leaves have activity on P. acnes on the 0 and 15th day of storage. The activities of ointment preparation on day 0 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 10.20 mm; 19.97 mm and 23.60 mm respectively, while the inhibition zones produced by the preparation of ointment on day 15 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 5.71 mm; 9.58 mm and 21.88 mm respectively.

Conclusion: Methanol extract of mango leaves (Mangifera indica L.) and ointment preparation can inhibit the growth of Propionibacterium acnes.

Keywords: Antibacterial agent, acne, Arum Manis mango leaves, MIC, Propionibacterium acnes, human pathogens.

Graphical Abstract

[1]
Brooks, G.F.; Butel, J.S.; Morse, S.A.; Mudihardi, E. Jawetz, Melnick, & Adelberg’s Mikrobiologi Kedokteran; Salemba Medika, 2005.
[2]
Kumaresan, A.; Deepthi, G.; Anandh, V.; Prathap, S. Effectiveness of positional release therapy in treatment of trapezitis. Int. J. Pharm. Sci. Health Care, 2012, 1, 71-81.
[3]
Bozin, B.; Mimica-Dukic, N.; Simin, N.; Anackov, G. Characterization of the volatile composition of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. J. Agric. Food Chem., 2006, 54(5), 1822-1828.
[http://dx.doi.org/10.1021/jf051922u] [PMID: 16506839]
[4]
Habbab, A.; Sekkoum, K.; Belboukhari, N.; Cheriti, A.; Aboul-Enein, H.Y. Essential oil chemical composition of. Curr. Bioact. Coumpounds, 2016, 12, 51-60.
[http://dx.doi.org/10.2174/1573407212666160330152633]
[5]
Gu, C.; Yang, M.; Zhou, Z.; Khan, A.; Cao, J.; Cheng, G. Purification and characterization of four benzophenone derivatives from Mangifera indica L. leaves and their antioxidant, immunosuppressive and α -glucosidase inhibitory activities. J. Funct. Foods, 2019, 52, 709-714.
[http://dx.doi.org/10.1016/j.jff.2018.11.045]
[6]
Engels, C.; Schieber, A.; Gänzle, M.G. Inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (Mangifera indica L.). Appl. Environ. Microbiol., 2011, 77(7), 2215-2223.
[http://dx.doi.org/10.1128/AEM.02521-10] [PMID: 21317249]
[7]
Gupta, C.; Garg, A.P.; Gupta, S. Antimicrobial and phytochemical studies of fresh ripe pulp and dried unripe pulp of Mangifera indica (AMCHUR). Middle East J. Sci. Res., 2010, 5, 75-80.
[8]
Pohan, A.P.N.; Purwaningsih, E.H.; Dwijayanti, A.; Hospital, M. The effect of mangiferin foetida leaves’ etanol extract on feritin serum of the patients suffering from Talasemia at Cipto Mangunkusumo Hospital. J. Ir. Med. Assoc., 2013, 1, 45-52.
[9]
Ningsih, D.R. Mango (Mangifera Indica L.) leave extract as antifungal agent to Candida albicans and its compound group identification. J. Kim. Ris., 2017, 2, 61-68.
[http://dx.doi.org/10.20473/jkr.v2i1.3690]
[10]
Rajan, S.; Suganya, H.; Thirunalasundari, T.; Jeeva, S. Antidiarrhoeal efficacy of Mangifera indica seed kernel on Swiss albino mice. Asian Pac. J. Trop. Med., 2012, 5(8), 630-633.
[http://dx.doi.org/10.1016/S1995-7645(12)60129-1] [PMID: 22840451]
[11]
Aliouche, L.; Larguet, H.; Amrani, A.; Brouard, I.; Benayache, S.; Zama, D.; Meraihi, Z.; Benayache, F. Isolation, antioxidant and antimicrobial activities of ecdysteroids from. Curr. Bioact. Compd., 2018, 14, 60-66.
[http://dx.doi.org/10.2174/1573407214666171211154922]
[12]
Ramesh Petchi, R.S.P.; Darwhekar, V.C.G.; GS, D. Antidiabetic effect of kernel seeds extract of Mangifera indica (Anacardiaceae). Int. J. Pharma Bio Sci. Res., 2011, 2, 385-393.
[13]
Ningsih, D.R. Zusfahair; Kartika, D.; Lestari, I.T. Synthesis of Anti-Acne Ointment of Ethanol Extract of White Plumeria Leaves. Plumeria Alba L.. IOP Conference Series: Materials Science and Engineering, Volume 172, 11th Joint Conference on Chemistry in Conjunction with the 4th Regional Biomaterials Scientific Meeting 15–16 September 2016, Purwokerto, Indonesia , 2017.172, 012013.
[14]
Ningsih, D.R.; Zusfahair, Z.; Purwati, P. Antibacterial activity cambodia leaf extract (Plumeria alba l.) to Staphylococcus aureus and identification of bioactive compound group of cambodia leaf extract. Molekul., 2014, 9, 101-109.
[http://dx.doi.org/10.20884/1.jm.2014.9.2.156]
[15]
Wasitaatmadja, S.M. Giude To Cosmetics; Univ. Indones: Jakarta, 1997.
[16]
Bayuaji, T.S.; Astuti, I.Y.; Dhiani, B.A. Antifungal activity of chinese ketepeng leaf cream (Senna alata L. Roxb.) to trichophyton mentagrophytes. Pharmacy (Basel), 2012, 9(3)
[17]
Lieberman, H.A.; Rieger, M.M.; Banker, G.S. Pharmaceutical Dosage Forms-Disperse Systems; Marcel Dekker: New York, 1989.
[http://dx.doi.org/10.1201/9781420000955]
[18]
Holetz, F.B.; Pessini, G.L.; Sanches, N.R.; Cortez, A.G.; Nakamura, C.V.; Prado, B.; Filho, D. Screening Pl medicinais 2.Pdf. Mem. Inst. Oswaldo Cruz, 2002, 97, 1027-1031.
[19]
Cushnie, T.P.T.; Lamb, A.J. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents, 2005, 26(5), 343-356.
[http://dx.doi.org/10.1016/j.ijantimicag.2005.09.002] [PMID: 16323269]
[20]
Siregar, A.F.; Sabdono, A.; Pringgenies, D. Antibacterial petential of seawed extract againts skin disease Pseudomonas aeruginosa. Staphylococcus epidermidis microccus luteus Bacteria. J. Marine Res.,, 2012, 1, 152-160.
[21]
Chauhan, N.; Tyagi, A.K.; Kumar, P.; Malik, A. Kumar, P.; Malik, A. Antibacterial potential of Jatropha curcas Synthesized silver nanoparticles against food borne pathogens. Front. Microbiol., 2016, 7, 1748.
[http://dx.doi.org/10.3389/fmicb.2016.01748] [PMID: 27877160]
[22]
Nuria, M.C. Antibacterial activity test of methanol extract of jatropha leaves (Jatropha Curcas L.) on Staphylococcus aureus ATCC 25923, Escherichia coli ATCC25922 and Salmonella typhi ATCC 1408. Bacteria, 2009, 5(2), 26-37.
[23]
Oprica, C.; Emtestam, L.; Lapins, J.; Borglund, E.; Nyberg, F.; Stenlund, K.; Lundeberg, L.; Sillerström, E.; Nord, C.E. Antibiotic-resistant Propionibacterium acnes on the skin of patients with moderate to severe acne in Stockholm. Anaerobe, 2004, 10(3), 155-164.
[http://dx.doi.org/10.1016/j.anaerobe.2004.02.002] [PMID: 16701513]
[24]
Nasional, D.S.N.; Standardisasi, D. Sun Block Dosages; Jakarta Board Natl. Stand, 1996.
[25]
Stiani, S.N.; Rumantir, R.; Megawati, S. Formulation of oinment etanol extract of Ocimum basilium L. leaves as anti-fungal agent with the oinment basic type variations and its physical evaluation characteristics. J. Farmagazine, 2016, 2, 1-5.

Rights & Permissions Print Cite
Article Metrics
6
© 2024 Bentham Science Publishers | Privacy Policy