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Current Protein & Peptide Science

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ISSN (Print): 1389-2037
ISSN (Online): 1875-5550

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Review Article

Detecting Protein-Protein Interaction Based on Protein Fragment Complementation Assay

Author(s): Tianwen Wang, Ningning Yang, Chen Liang, Hongjv Xu, Yafei An, Sha Xiao, Mengyuan Zheng, Lu Liu, Gaozhan Wang and Lei Nie*

Volume 21, Issue 6, 2020

Page: [598 - 610] Pages: 13

DOI: 10.2174/1389203721666200213102829

Price: $65

Abstract

Proteins are the most critical executive molecules by responding to the instructions stored in the genetic materials in any form of life. More frequently, proteins do their jobs by acting as a roleplayer that interacts with other protein(s), which is more evident when the function of a protein is examined in the real context of a cell. Identifying the interactions between (or amongst) proteins is very crucial for the biochemistry investigation of an individual protein and for the attempts aiming to draw a holo-picture for the interacting members at the scale of proteomics (or protein-protein interactions mapping). Here, we introduced the currently available reporting systems that can be used to probe the interaction between candidate protein pairs based on the fragment complementation of some particular proteins. Emphasis was put on the principles and details of experimental design. These systems are dihydrofolate reductase (DHFR), β-lactamase, tobacco etch virus (TEV) protease, luciferase, β- galactosidase, GAL4, horseradish peroxidase (HRP), focal adhesion kinase (FAK), green fluorescent protein (GFP), and ubiquitin.

Keywords: Protein-protein interaction, protein complementation assay, interaction reporting system, enzyme, fluorescent protein, ubiquitin.

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