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Infectious Disorders - Drug Targets

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ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

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Research Article

Evaluation of Putative Toxin-antitoxins Systems in Clinical Brucella melitensis in Iran

Author(s): Safoura Moradkasani, Ebrahim Kouhsari, Faramarz Masjedian Jazi, Behrooz Sadeghi Kalani and Iraj Pakzad*

Volume 21, Issue 1, 2021

Published on: 17 January, 2020

Page: [38 - 42] Pages: 5

DOI: 10.2174/1871526520666200117113420

Price: $65

Abstract

Background: Toxin-antitoxin systems (TAs) are two-component elements, which are extensive in the bacterial genome and have a regulatory role in many cellular activities including, growth arrest, survival, biofilm formation, and bacterial persistence.

Objective: TAs have not well studied in Brucella spp.

Methods: We evaluated the presence of different toxin-antitoxin systems, including relE- rhhlike, Fic- Phd, Cog- Rhh, and cogT- cogAT in 40 clinical Brucella melitensis isolates using PCRbased sequencing assay.

Results: Our results showed the high presence of relE-rhh-like, Fic-Phd, Cog-rhh, and cogTcogAT s TAs genes in B. melitensis isolates that were 96.25%, 92.5%, 96.25%, and 95%, respectively.

Conclusion: A high presence of TAs genes in clinical B. melitensis isolates revealed that the TA system could be an antibacterial target in B. melitensis but more investigation is necessitated to elucidate the exact roles of these genes.

Keywords: Brucella melitensis, toxin-antitoxin systems, relE- rhh-like, Fic- Phd, Cog- Rhh, cogT- cogAT.

Graphical Abstract

[1]
Lai, S.; Zhou, H.; Xiong, W.; Gilbert, M.; Huang, Z.; Yu, J.; Yin, W.; Wang, L.; Chen, Q.; Li, Y.; Mu, D.; Zeng, L.; Ren, X.; Geng, M.; Zhang, Z.; Cui, B.; Li, T.; Wang, D.; Li, Z.; Wardrop, N.A.; Tatem, A.J.; Yu, H. Changing epidemiology of human brucellosis, China, 1955–2014. Emerg. Infect. Dis., 2017, 23(2), 184-194.
[http://dx.doi.org/10.3201/eid2302.151710] [PMID: 28098531]
[2]
Tuon, F.F.; Gondolfo, R.B.; Cerchiari, N. Human-to-human transmission of Brucella - a systematic review. Trop. Med. Int. Health, 2017, 22(5), 539-546.
[http://dx.doi.org/10.1111/tmi.12856] [PMID: 28196298]
[3]
de Figueiredo, P.; Ficht, T.A.; Rice-Ficht, A.; Rossetti, C.A.; Adams, L.G. Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions. Am. J. Pathol., 2015, 185(6), 1505-1517.
[http://dx.doi.org/10.1016/j.ajpath.2015.03.003] [PMID: 25892682]
[4]
Makarova, K.S.; Wolf, Y.I.; Koonin, E.V. Comprehensive comparative-genomic analysis of type 2 toxin-antitoxin systems and related mobile stress response systems in prokaryotes. Biol. Direct, 2009, 4(1), 19.
[http://dx.doi.org/10.1186/1745-6150-4-19] [PMID: 19493340]
[5]
Yamaguchi, Y.; Park, J-H.; Inouye, M. Toxin-antitoxin systems in bacteria and archaea. Annu. Rev. Genet., 2011, 45, 61-79.
[http://dx.doi.org/10.1146/annurev-genet-110410-132412] [PMID: 22060041]
[6]
Leplae, R.; Geeraerts, D.; Hallez, R.; Guglielmini, J.; Drèze, P.; Van Melderen, L. Diversity of bacterial type II toxin-antitoxin systems: a comprehensive search and functional analysis of novel families. Nucleic Acids Res., 2011, 39(13), 5513-5525.
[http://dx.doi.org/10.1093/nar/gkr131] [PMID: 21422074]
[7]
Diaz-Orejas, R.; Diago-Navarro, E.; Arriaga, A.M.H.; Lopez-Villarejo, J.; Lemonnier, M.; Moreno-Cordoba, I. Bacterial toxin-antitoxin systems targeting translation. J. Appl. Biomed., 2010, 8(4), 179-188.
[http://dx.doi.org/10.2478/v10136-009-0021-9]
[8]
Page, R.; Peti, W. Toxin-antitoxin systems in bacterial growth arrest and persistence. Nat. Chem. Biol., 2016, 12(4), 208-214.
[http://dx.doi.org/10.1038/nchembio.2044] [PMID: 26991085]
[9]
Goeders, N.; Van Melderen, L. Toxin-antitoxin systems as multilevel interaction systems. Toxins (Basel), 2014, 6(1), 304-324.
[http://dx.doi.org/10.3390/toxins6010304] [PMID: 24434905]
[10]
Hu, M-x.; Zhang, X.; Li, E-l.; Feng, Y-J. Recent advancements in toxin and antitoxin systems involved in bacterial programmed cell death. Int. J. Microbiol., 2010, 2010 781430
[11]
Lee, K-Y.; Lee, B-J. Structure, biology, and therapeutic application of toxin–antitoxin systems in pathogenic bacteria. Toxins (Basel), 2016, 8(10), 305.
[http://dx.doi.org/10.3390/toxins8100305] [PMID: 27782085]
[12]
Ali, S.; Ali, Q.; Melzer, F.; Khan, I.; Akhter, S.; Neubauer, H.; Jamal, S.M. Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR. Trop. Anim. Health Prod., 2014, 46(1), 73-78.
[http://dx.doi.org/10.1007/s11250-013-0448-6] [PMID: 23868548]
[13]
Bouza, E.; Sánchez-Carrillo, C.; Hernangómez, S.; González, M.J. Spanish Co-operative Group for the Study of Laboratory-acquired Brucellosis. Laboratory-acquired brucellosis: a Spanish national survey. J. Hosp. Infect., 2005, 61(1), 80-83.
[http://dx.doi.org/10.1016/j.jhin.2005.02.018] [PMID: 16130212]
[14]
Gupta, V.; Verma, D.K.; Singh, K.; Kumari, R.; Singh, S.; Vihan, V. Single-step PCR for detection of Brucella melitensis from tissue and blood of goats. Small Rumin. Res., 2006, 66(1-3), 169-174.
[http://dx.doi.org/10.1016/j.smallrumres.2005.09.001]
[15]
Xie, Y.; Wei, Y.; Shen, Y.; Li, X.; Zhou, H.; Tai, C.; Deng, Z.; Ou, H.Y. TADB 2.0: an updated database of bacterial type II toxin-antitoxin loci. Nucleic Acids Res., 2018, 46(D1), D749-D753.
[http://dx.doi.org/10.1093/nar/gkx1033] [PMID: 29106666]
[16]
Sevin, E.W.; Barloy-Hubler, F. RASTA-Bacteria: a web-based tool for identifying toxin-antitoxin loci in prokaryotes. Genome Biol., 2007, 8(8), R155.
[http://dx.doi.org/10.1186/gb-2007-8-8-r155] [PMID: 17678530]
[17]
Mirkalantari, S.; Amirmozafari, N.; Kazemi, B.; Irajian, G. Molecular cloning of virB12 gene of Brucella melitensis 16M strain in pET28a vector. Asian Pac. J. Trop. Med., 2012, 5(7), 511-513.
[http://dx.doi.org/10.1016/S1995-7645(12)60089-3] [PMID: 22647810]
[18]
Zowghi, E.; Ebadi, A.; Yarahmadi, M. Isolation and identification of Brucella organisms in Iran. Iran. J. Clin. Infect. Dis., 2008, 3(4), 185-188.
[19]
Mirnejad, R.; Jazi, F.M.; Mostafaei, S.; Sedighi, M. Epidemiology of brucellosis in Iran: A comprehensive systematic review and meta-analysis study. Microb. Pathog., 2017, 109, 239-247.
[http://dx.doi.org/10.1016/j.micpath.2017.06.005] [PMID: 28602839]
[20]
Kędzierska, B.; Hayes, F. Emerging roles of toxin-antitoxin modules in bacterial pathogenesis. Molecules, 2016, 21(6), 790.
[http://dx.doi.org/10.3390/molecules21060790] [PMID: 27322231]

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