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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Antimicrobial Effect of Quercus robur L. Leaves Selective Extracts from the Mezi Mountain of Djeniene Bourezg (West of Algeria)

Author(s): Elhassan Benyagoub*, Nouria Nabbou and Amal Dine

Volume 16, Issue 8, 2020

Page: [1181 - 1190] Pages: 10

DOI: 10.2174/1573407216666191226141609

Price: $65

Abstract

Background: Algeria, by its vast terrestrial extent and its climatic variation, has an abundant, rich and varied flora in which it was counted many aromatic and medicinal species that provide bioactive compounds characterized by their broad biological activities. In this context, this work is based on the evaluation of the antimicrobial activity of Quercus robur L. leaves extracts (Family of Fagaceae).

Methods: Firstly, the collected plant material was defatted; then, the extraction of tannins and saponins was carried out according to a standard protocol where the extracts obtained were tested on some uropathogenic microbial strains by disk diffusion method with the determination of the Minimum Inhibitory Concentrations (MICs) by broth macro-dilution method.

Results: The extraction yield of the selective extracts was 7.93 and 16.94% for tannins and saponins, respectively. The antibiotic resistance profile of the tested strains showed a resistance relatively important to several antibiotics, namely amoxicillin +clavulanic acid and ampicillin for Escherichia coli, while Pseudomonas aeruginosa showed resistance to amoxicillin+clavulanic acid, amikacin, cefotaxime and ceftazidime. However, Staphylococcus aureus was susceptible to penicillin, gentamicin, ofloxacin and chloramphenicol. Antifungal susceptibility testing has been shown that Candida albicans was susceptible to amphotericin B, econazole and it was clinically categorized as intermediate to miconazole drug. For antimicrobial tests, the tannins and saponins extracts exhibited a low to strong inhibitory effect at tested concentrations lower than 30 mg/mL (ranged from no inhibition to an inhibition zone diameter of 17.5 mm), depending on dose levels and tested microbial strains.

Conclusion: This activity is proportional to the tested concentrations, knowing that tannins extract was more active compared to saponins extract. For this, Q. robur could constitute an important source for drug discovery.

Keywords: Tannins, saponins, Quercus robur L. leaves, antimicrobial activity, uropathogenic strains, MIC.

Graphical Abstract

[1]
Riwom Essama, S.H.; Nyegue, M.A. Ndoye, F C.; Kamga, S.K.; Bouopda, T.S.P.; Etoa, F.X. Antibacterial and antioxidant activities of Hydro-ethanol extracts of barks, leaves and stems of Annona muricata. Am. J. Pharmacol. Sci., 2015, 3(6), 126-131.
[2]
Yakhlef, G. Study of the biological activity of extracts from the leaves of Thymus vulgaris L. and Laurus nobilis L.; Master thesis in Applied Biochemistry, El Hadj Lakhdar Batna University, Faculty of Sciences, Department of Biology: Algeria. 2010.
[3]
Bahorun, T.; Gressier, B.; Trotin, F.; Brunet, C.; Dine, T.; Luyckx, M.; Vasseur, J.; Cazin, M.; Cazin, J.C.; Pinkas, M. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung,, 1996, 46(11), 1086-1089.https://www.ncbi.nlm.nih.gov/pubmed/8955870#
[http://dx.doi.org/8955870]
[4]
Ivanenkov, Y.A.; Chufarova, N.V.; Veselov, M.S.; Majouga, A.G. Dietary polyphenols for prostate cancer therapy. Curr. Bioact. Compd., 2014, 10(2), 76-111.
[http://dx.doi.org/10.2174/157340721002141001102524]
[5]
OMS/WHO. The world health report 1998 - Life in the 21st century: A vision for all 1998.http://www.who.int/whr/1998/en/
[6]
Akharaiyi, F.C.; Boboye, B. Antibacterial and phytochemical evaluation of three medicinal plants. J. Nat. Prod., 2010, 3, 27-34.http://journalofnaturalproducts.com/Volume3.html
[7]
Jamet, R.; Beauvais, M.; Liska, P.; Waldhauserova, A. Plantes médicinales; Grund, L.P.C., Ed.; ISBN 2006.https://www.leslibraires.fr/livre/586271-plantes-medicinales-petr-liska-sylva-francova-andrea-waldhauserova-grund
[8]
Nemlin, J.; Brunel, J.F. Practical work booklet on Matière Médicale (3rd year). National University of Côte-d'Ivoire; Pharmacological faculty. Department of Pharmacognosy. Phytology laboratory, 1995.
[9]
El-Hela, A.A.; Abdelhady, N.M.; El-Hefnawy, H.M.; Ibrahim, T.A.; Abdallah, G.M. Anti-inflammatory effect, antioxidant potentials and phytochemical investigation of Crataegus sinaica boiss. roots growing in Egypt. Eur. J. Pharm. Med. Res., 2016, 3(11), 128-137.http://www.ejpmr.com/home/abstract_id/1653
[10]
Bruneton, J. Pharmacognosie, phytochimie, plantes médicinales. 5th ed; Technique et Documentation Lavoisier, Paris: Éditions Lavoisier; , 1999. https://www.decitre.fr/livres/pharmacognosie-9782743003159.html#resume
[11]
Okwus, D.E. Phytochemicals, vitamins and mineral contents of two Nigeria medicinal plants. Int. J. Mol. Adv. Sci, 2005, 1(4), 375-381.
[http://dx.doi.org/medwelljournals.com/abstract/?doi=ijmmas.2005.375.381]
[12]
CLSI Performance Standards for Antimicrobial Disk Susceptibility Testing M100. 28th ed., Wayne Pennsylvania 19087-USA; , 2018.
[13]
Sacchetti, G.; Maietti, S.; Murroli, M.; Scagliant, M.; Mansredini, S.; Radice, M. Comparative evaluation of 11 essentials oils different origin as functional antioxydants, antiradicals and antimicrobial in food. Food Chem., 2005, 91(4), 621-632.
[http://dx.doi.org/10.1016/j.foodchem.2004.06.031]
[14]
Celiktas, O.; Hames, E.; Bedir, E.; Vardar, S. Antimicrobiol activities of methanol extracts and essential oils of Rosmarinus officinalis depending on location and seasonal variations. Food Chem., 2007, 100(2), 553-559.
[http://dx.doi.org/10.1016/j.foodchem.2005.10.011]
[15]
CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, approved standard CLSI document M07-A9; 9th Clinical and Laboratory Standards Institute: 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, , 2012.
[16]
Balouiri, M.; Sadiki, M.; Ibnsouda, S.K. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal., 2016, 6(2), 71-79.
[http://dx.doi.org/10.1016/j.jpha.2015.11.005] [PMID: 29403965]
[17]
Singh, K. Phytochemical determination and antibacterial activity of Trichosanthes dioica Roxb (Patal), Cucurbita maxima (pumpkin) and Abelmoschus esculentus Moench (Okra) plant seeds. Master Dissertation in life sciences, Department of Life Science, National Institute of Technology Rourkela: Odisha (India),. 2012.
[18]
Mladenovic, N.; Mladenovic, A.; Pavlovic, S.; Baskic, D.; Zdravkovic, N. The aqueous extract of Quercus robur L. (Fagaceae) Shows promising antibacterial activity against Klebsiella pneumoniae. Global J. Pathol. Microbiol., 2014, 2(2), 53-58.
[19]
Tassouiket, S. Sensibilité aux antibiotiques d’Escherichia coli isolés d’infection urinaires communautaires à l’institut Pasteur de Casablanca Thèse de Doctorat en Pharmacie: Maroc, 2014.http://ao.um5s.ac.ma/xmlui/handle/123456789/14701
[20]
Chandra, H.; Bishnoi, P.; Yadav, A.; Patni, B.; Mishra, A.P.; Nautiyal, A.R. Antimicrobial resistance and the alternative resources with special emphasis on plant-based antimicrobials- A review. Plants (Basel), 2017, 6(2), 1-11.
[http://dx.doi.org/10.3390/plants6020016] [PMID: 28394295]
[21]
Benyagoub, E. Benyagoub, El-H; Berbaoui, H; Rahmani, C; Benyoucef, L. Identification and study of the emergence of antibiotic resistance of microorganisms responsible for urinary tract infections in Bechar (Algeria). ScienceLib, 2013, 5, 1-13.
[22]
Pappas, P.G.; Rex, J.H.; Sobel, J.D.; Filler, S.G.; Dismukes, W.E.; Walsh, T.J.; Edwards, J.E. Infectious Diseases Society of America. Guidelines for treatment of candidiasis. Clin. Infect. Dis., 2004, 38(2), 161-189.
[http://dx.doi.org/10.1086/380796] [PMID: 14699449]
[23]
Benyagoub, E.; Nabbou, N.; Boukhalkhel, S.; Dehini, I. The in vitro evaluation of the antimicrobial activity of Quercus robur L. methanolic and aqueous leaves’ extracts, from the Algerian high plateaus against some uro-pathogenic microbial strains; Phytother, 2019.
[24]
Ashok, P.K.; Upadhyaya, K. Tannins are Astringent. J. Pharmacogn. Phytochem., 2012, 1(3), 45-50.
[http://dx.doi.org/www.phytojournal.com/archives/?year=2012&vol=1&issue=3&ArticleId=21]
[25]
Nicolas, J.P. Pharmacopée traditionnelle des MAYA K’ICHÉ: étude ethnopharmacologique et système de classification indigène. Fait partie d'un numéro thématique. Annuaire. Tome, 1997, 106, 1997-1998.
[http://dx.doi.org/10.3406/ephe.1997.12825]
[26]
Lassus Saint-Geniès, G. Les vertus du Chêne (Quercus robur). La plume de l’Oiseau-Lyre, 2013 [Accessed on 10th Aug 2018]. 2013.http://laplumedeloiseaulyre.com/?p=548#respond
[27]
Min, B.R.; Pinchak, W.E.; Merkel, R.; Walker, S.; Tomita, G.; Anderson, R.C. Comparative antimicrobial activity of tannin extracts from perennial plants on mastitis pathogens. Sci. Res. Essays, 2008, 3(2), 66-73.
[http://dx.doi.org/www.academicjournals.org/journal/SRE/article-abstract/B16025414213]
[28]
Scalbert, A. Antimicrobial properties of tannins. Phytochemistry, 1991, 30(12), 3875-3883.
[http://dx.doi.org/10.1016/0031-9422(91)83426-L]
[29]
Djahra, A.B.; Bordjiba, O.; Benkherara, S. Extraction, séparation et activité antibactérienne des tanins de marrube blanc (Marrubium vulgare L.). Phytotherapie, 2013, 11(6), 348-352.
[http://dx.doi.org/10.1007/s10298-013-0819-1]
[30]
Krzyzowska, M.; Tomaszewska, E.; Ranoszek-Soliwoda, K.; Bien, K.; Orlowski, P.; Celichowski, G.; Grobelny, J. Tannic acid modification of metal nanoparticles: possibility for new antiviral applications. Nanostructures for Oral Medicine; Andronescu, E; Andronescu, A. M Micro and Nano Technologies. Romania, Elsevier;; , 2017, pp. 335-363.
[http://dx.doi.org/10.1016/B978-0-323-47720-8.00013-4]
[31]
Kouamé, J.; Gnoula, C.; Palé, E.; Bassolé, H.; Guissou, I.P.; Simporé, J.; Nikiéma, J-B. Etude des propriétés cytotoxiques et anti-radicalaires d’extraits de feuilles et de galles de Guiera senegalensis J. F. Gmel (Combretacae) Etude des propriétés cytotoxiques et anti-radicalaires d’extraits de feuilles et de galles de Guiera senegalensis J. F. Gmel (Combretacae), 2009, 32(1-2), 9-23.
[http://dx.doi.org/labiogene.org/spip.php?article152]
[32]
Benahmed-Djilali, A; Chemoul, T; Kal, S; Nabiev, M; Besombes, C Propriétés d’une pommade antibactérienne formulée à base de saponines extraites des feuilles de noyer. Phytother (Springer nature),, 2017, 1-9.
[33]
Pérez, A.J.; Pecio, Ł.; Kowalczyk, M.; Kontek, R.; Gajek, G.; Stopinsek, L.; Mirt, I.; Oleszek, W.; Stochmal, A. Triterpenoid Components from Oak Heartwood (Quercus robur) and their potential health benefits. J. Agric. Food Chem., 2017, 65(23), 4611-4623.
[http://dx.doi.org/10.1021/acs.jafc.7b01396] [PMID: 28535679]
[34]
Tayel, A.A.; El-Sedfy, M.A.; Ibrahim, A.I.; Moussa, S.H. Application of Quercus infectoria extract as a natural antimicrobial agent for chicken egg decontamination. Rev. Argent. Microbiol., 2018, 50(4), 391-397.
[http://dx.doi.org/10.1016/j.ram.2017.12.003] [PMID: 29691109]
[35]
Arabski, M.; Wasik, S.; Dworecki, K.; Kaca, W. Laser interferometric and cultivation methods for measurement of colistin/ampicilin and saponin interactions with smooth and rough of Proteus mirabilis lipopolysaccharides and cells. J. Microbiol. Methods 2009, 72(2), 178-183.https://www.ncbi.nlm.nih.gov/pubmed/19318050
[http://dx.doi.org/10.1016/j.mimet.2009.01.020] [PMID: 19318050]
[36]
Arabski, M.; Węgierek-Ciuk, A.; Czerwonka, G.; Lankoff, A.; Kaca, W. Effects of saponins against clinical E. coli strains and eukaryotic cell line. J. Biomed. Biotechnol., 2012, 2012,286216.
[http://dx.doi.org/10.1155/2012/286216] [PMID: 22500084]

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