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Current Analytical Chemistry

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ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

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Research Article

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

Author(s): Laleh Samini, Maryam Khoubnasabjafari, Mohamad M. Alimorad, Vahid Jouyban-Gharamaleki, Hak-Kim Chan and Abolghasem Jouyban*

Volume 16, Issue 8, 2020

Page: [1032 - 1040] Pages: 9

DOI: 10.2174/1573411015666191203104522

Price: $65

Abstract

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations.

Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS.

Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples.

Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

Keywords: Bioanalysis, budesonide, determination, exhaled breath condensate, HPLC-UV, microextraction.

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Graphical Abstract

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