Abstract
Background: H+/K+ ATPase a protein present in the gastric parietal cells is a better target for the prevention and treatment of gastric ulcer. Plant flavonoids have been reported to elicit anti-ulcer activity by inhibiting the proton pump as well as by antioxidant defense mechanism.
Methods: Chloroform fraction of hydro-alcoholic extract of passion fruit was screened for proton pump inhibitory assay using goat parietal cell. In-silico computational docking studies were carried out using Glide program in order to validate the inhibitory action of selected constituents.
Results: The flavonoid rich fruit possess a promising radical scavenging activity against DPPH. 10.41μg/mL is sufficient to inhibit 50% of ATPase enzyme activity. A synergistic activity was also achieved by the fruit with sub-effective doses of lansoprazole. Fenton's oxidation induced by H2O2 was also blunted by the fruit extract.
Conclusion: The in-vitro and in-silico findings indicated that, passion fruit can be a good dietary supplement for the prevention and management of ulcer.
Keywords: Passion fruit, ulcer, H+/K+ ATPase, flavonoids, oxidation, glide program.
Graphical Abstract
[1]
Jcetendra, K.G.; Neeraj, U.; Patnaik, A.C.; Papiya, M.M. Evaluation of Anti-ulcer activity of Leucas lavandulifalia on Mucosal lesion in rat. Asian J. Pharma. Clin. Res., 2010, 3(2), 110-120.
[2]
Sachs, G.; Maton, P.N.; Wallmark, B. Pharmacology of Peptic ulcer disease; Springer Verlag: New York, 1991.
[3]
Chan, F.K.; Leung, W.K. Peptic-ulcer disease. Lancet, 2002, 360(9337), 933-941.
[http://dx.doi.org/10.1016/S0140-6736(02)11030-0] [PMID: 12354485]
[http://dx.doi.org/10.1016/S0140-6736(02)11030-0] [PMID: 12354485]
[4]
Freitas, C.S.; Baggio, C.H.; Finau, J.; Anginoni, M.; Pizzolatti, M.G.; Santos, A.R.; Marques, M.C.; Maria, C.A. Inhibition of H+/K+ ATPase in the gastroprotective effect of Baccharis illinita DC. J. Pharm. Pharmacol., 2008, 60(8), 1105-1110.
[http://dx.doi.org/10.1211/jpp.60.8.0017] [PMID: 18644203]
[http://dx.doi.org/10.1211/jpp.60.8.0017] [PMID: 18644203]
[5]
Yao, X.; Forte, J.G. Cell biology of acid secretion by the parietal cell. Annu. Rev. Physiol., 2003, 65, 103-131.
[http://dx.doi.org/10.1146/annurev.physiol.65.072302.114200] [PMID: 12500969]
[http://dx.doi.org/10.1146/annurev.physiol.65.072302.114200] [PMID: 12500969]
[6]
Yang, D.H.; Tsuyama, S.; Murata, F. The expression of gastric H+-K+-ATPase mRNA and protein in developing rat fundic gland. Histochem. J., 2001, 33(3), 159-166.
[http://dx.doi.org/10.1023/A:1017948126730] [PMID: 11508339]
[http://dx.doi.org/10.1023/A:1017948126730] [PMID: 11508339]
[7]
Halliwell, B.; Gutteridge, J.M.C. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch. Biochem. Biophys., 1986, 246(2), 501-514.
[http://dx.doi.org/10.1016/0003-9861(86)90305-X] [PMID: 3010861]
[http://dx.doi.org/10.1016/0003-9861(86)90305-X] [PMID: 3010861]
[8]
Tandon, R.; Mukherjee, N.; Dixit, V.K.; Khanna, R.; Khanna, H.D. Lipid peroxidation levels in peptic ulcer and gastric carcinoma. Indian J. Physiol. Pharmacol., 2006, 50(1), 83-86.
[PMID: 16850909]
[PMID: 16850909]
[10]
Harborne, J.B. Phytochemical method- A guide to modern technical of plant analysis, 3rd ed; Chapman and Hall: London, 1998.
[11]
Patel, A.; Patel, A.; Patel, A. Dr. N. M. Patel. Estimation of Flavonoid, PolyphenolicContent and In-vitro AntioxidantCapacity of leaves of Tephrosia purpureaLinn. (Leguminosae). Int. J. Pharm. Sci. Res., 2010, 1(1), 66-77.
[12]
Doshi, G.M.; Une, H.D. Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography. Pharmacognosy Res., 2016, 8(1), 37-42.
[http://dx.doi.org/10.4103/0974-8490.171098] [PMID: 26941534]
[http://dx.doi.org/10.4103/0974-8490.171098] [PMID: 26941534]
[13]
Govindan, P.; Muthukrishnan, S. Evaluation of total phenolic content and free radical scavenging activity of Boerhavia erecta. J Acute Med., 2013, 3(3), 103-109.
[http://dx.doi.org/10.1016/j.jacme.2013.06.003]
[http://dx.doi.org/10.1016/j.jacme.2013.06.003]
[14]
Priyanka, Y.; Aditya, G.; Gopal, R. In vitro H+-K+ ATPase inhibitory potential of methanolic extract of Cissus quadrangularisLinn. Pharmacol. Res., 2012, 4(2), 123-126.
[15]
Zeraik, M.L.; Yariwake, J.H. Quantification of isoorientin and total flavonoids in Passiflora edulis fruit pulp. Microchem. J., 2010, 96(1), 86-91.
[http://dx.doi.org/10.1016/j.microc.2010.02.003]
[http://dx.doi.org/10.1016/j.microc.2010.02.003]
[16]
Li, H.; Zhou, P.; Yang, Q.; Shen, Y.; Deng, J.; Li, L.; Zhao, D. Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis ‘edulis’ and Passiflora edulis ‘flavicarpa’. J. Ethnopharmacol., 2011, 133(3), 1085-1090.
[http://dx.doi.org/10.1016/j.jep.2010.11.039] [PMID: 21111038]
[http://dx.doi.org/10.1016/j.jep.2010.11.039] [PMID: 21111038]
[17]
Shinoda, T.; Ogawa, H.; Cornelius, F.; Toyoshima, C. Crystal structure of the sodium-potassium pump at 2.4 A resolution. Nature, 2009, 459(7245), 446-450.
[http://dx.doi.org/10.1038/nature07939] [PMID: 19458722]
[http://dx.doi.org/10.1038/nature07939] [PMID: 19458722]
[18]
Lowry, O.H.; Rosenbourgh, N.J.; Randall, R.J. Neutraceutical properties of agro waste part of donageczi. J. Biochem., 1951, 193, 65.
[19]
Bellé, N.A.V.; Dalmolin, G.D.; Fonini, G.; Rubin, M.A.; Rocha, J.B. Polyamines reduces lipid peroxidation induced by different pro-oxidant agents. Brain Res., 2004, 1008(2), 245-251.
[http://dx.doi.org/10.1016/j.brainres.2004.02.036] [PMID: 15145762]
[http://dx.doi.org/10.1016/j.brainres.2004.02.036] [PMID: 15145762]
[20]
Ohkawa, H.; Ohishi, N.; Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 1979, 95(2), 351-358.
[http://dx.doi.org/10.1016/0003-2697(79)90738-3] [PMID: 36810]
[http://dx.doi.org/10.1016/0003-2697(79)90738-3] [PMID: 36810]
[21]
Kade, I.J.; Ibukun, E.O.; Nogueira, C.W.; da Rocha, J.B.T. Sun-drying diminishes the antioxidative potentials of leaves of Eugenia uniflora against formation of thiobarbituric acid reactive substances induced in homogenates of rat brain and liver. Exp. Toxicol. Pathol., 2008, 60(4-5), 365-371.
[http://dx.doi.org/10.1016/j.etp.2007.12.001] [PMID: 18539016]
[http://dx.doi.org/10.1016/j.etp.2007.12.001] [PMID: 18539016]
[22]
Megha, K.J.; Hareeshma, C. Babu, Sree Kutty MV, Kanthlal S K. Polyphenol rich passion fruit inhibits Fenton’s reagent induced lipid peroxidation in rabbit colon. Int J Pharm & Tech., 2016, 8(4), 25158-25163.
[23]
Gadekar, R.; Singour, P.K.; Chaurasiya, P.K.; Pawar, R.S.; Patil, U.K. A potential of some medicinal plants as an antiulcer agents. Pharmacogn. Rev., 2010, 4(8), 136-146.
[http://dx.doi.org/10.4103/0973-7847.70906] [PMID: 22228953]
[http://dx.doi.org/10.4103/0973-7847.70906] [PMID: 22228953]
[24]
Jorge, R.M.; Leite, J.P.; Oliveira, A.B.; Tagliati, C.A. Evaluation of antinociceptive, anti-inflammatory and antiulcerogenic activities of Maytenus ilicifoliaJ. J. Ethnopharmacol., 2004, 94(1), 93-100.
[25]
Shukla, A.; Verma, S.; Bishnoi, R.; Jain, C.P. In vitro Carissa H-K ATPase inhibitory potential of methanolic extract of Carissa carandas Linn. Leaves. Asian J Pharm Pharmacol, 2016, 2(5), 132-135.
[26]
Lindström, E.; Chen, D.; Norlén, P.; Andersson, K.; Håkanson, R. Control of gastric acid secretion:the gastrin-ECL cell-parietal cell axis. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 2001, 128(3), 505-514.
[http://dx.doi.org/10.1016/S1095-6433(00)00331-7] [PMID: 11246041]
[http://dx.doi.org/10.1016/S1095-6433(00)00331-7] [PMID: 11246041]
[27]
Prakash Gupta, R.K. Pradeepa; Hanumanthappa, M. In vitro antioxidant and H(+), K(+)-ATPase inhibition activities of Acalypha wilkesiana foliage extract. J. Pharm. Bioallied Sci., 2013, 5(3), 214-223.
[http://dx.doi.org/10.4103/0975-7406.116822] [PMID: 24082698]
[http://dx.doi.org/10.4103/0975-7406.116822] [PMID: 24082698]
[28]
Prabhakar, V.; Iqbal, H.; Balasubramanian, R. Antioxidant studies on monosubstituted chalcone derivatives - understanding substituent effects. Pak. J. Pharm. Sci., 2016, 29(1), 165-171.
[PMID: 26826830]
[PMID: 26826830]
[29]
Tandon, R.; Mukherjee, N.; Dixit, V.K.; Khanna, R.; Khanna, H.D. Lipid peroxidation levels in peptic ulcer and gastric carcinoma. Indian J. Physiol. Pharmacol., 2006, 50(1), 83-86.
[PMID: 16850909]
[PMID: 16850909]
[30]
Tandon, R.; Khanna, H.D.; Dorababu, M.; Goel, R.K. Oxidative stress and antioxidants status in peptic ulcer and gastric carcinoma. Indian J. Physiol. Pharmacol., 2004, 48(1), 115-118.
[PMID: 15270379]
[PMID: 15270379]
[31]
Viswanad, V.; Aleykutty, N.A.; Zacharia, S.M.; Thomas, L. Evaluation of antioxidant and free radical scavenging activity of Samadera indica using In vitro models. Pharmacogn. J., 2011, 3(23), 85-90.
[http://dx.doi.org/10.5530/pj.2011.23.13]
[http://dx.doi.org/10.5530/pj.2011.23.13]
[32]
Ajaikumar, K.B.; Asheef, M.; Babu, B.H.; Padikkala, J. The inhibition of gastric mucosal injury by Punicagranatum L. (pomegranate) methanolic extract. J. Ethnopharmacol., 2005, 96(1-2), 171-176.
[http://dx.doi.org/10.1016/j.jep.2004.09.007] [PMID: 15588667]
[http://dx.doi.org/10.1016/j.jep.2004.09.007] [PMID: 15588667]
[33]
Ishige, K.; Schubert, D.; Sagara, Y. Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Radic. Biol. Med., 2001, 30(4), 433-446.
[http://dx.doi.org/10.1016/S0891-5849(00)00498-6] [PMID: 11182299]
[http://dx.doi.org/10.1016/S0891-5849(00)00498-6] [PMID: 11182299]
[34]
La Casa, C.; Villegas, I.; Alarcón de la Lastra, C.; Motilva, V.; Martín Calero, M.J. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J. Ethnopharmacol., 2000, 71(1-2), 45-53.
[http://dx.doi.org/10.1016/S0378-8741(99)00174-9] [PMID: 10904145]
[http://dx.doi.org/10.1016/S0378-8741(99)00174-9] [PMID: 10904145]
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