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Research Article

丁酸对IPEC-J2细胞分化，增殖，凋亡和自噬的影响

卷 20, 期 4, 2020

页: [307 - 317] 页: 11

弟呕挨: 10.2174/1566524019666191024110443

价格: $65

摘要

背景：短链脂肪酸丁酸（BT）是首选的结肠细胞能源。尚未完全阐明BT对仔猪小肠上皮细胞的分化，增殖和凋亡的影响及其潜在机制。 方法：在这项研究中，发现0.2-0.4 mM BT促进了前列腺空肠上皮（IPEC-J2）细胞的分化。 0.5 mM或更高浓度的BT以剂量和时间依赖性方式显着损害细胞活力。此外，高浓度的BT抑制IPEC-J2细胞增殖并诱导G2 / M期细胞周期停滞。 结果：我们的结果表明，BT通过caspase8-caspase3途径触发IPEC-J2细胞凋亡，并伴有过量的活性氧（ROS）和TNF-α的产生。高浓度的BT抑制了与溶酶体形成增加有关的细胞自噬。已发现，p38 MAPK抑制剂SB202190可减弱BT降低的IPEC-J2细胞活力。此外，SB202190减弱了负责正常酸性溶酶体的BT增加的p38 MAPK靶标DDIT3 mRNA水平和V-ATPase mRNA水平。 结论：总而言之，1）BT在0.2-0.4 mM时促进IPEC-J2细胞的分化； 2）0.5 mM或更高浓度的BT通过p38 MAPK途径诱导细胞凋亡； 3）BT抑制细胞自噬并在高浓度下促进溶酶体形成。

关键词: 丁酸，p38 MAPK，凋亡，自噬，V-ATPase，溶酶体。

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