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Research Article

吗啡对家兔结肠和小肠运输中Cajal间质细胞影响的实验研究

卷 20, 期 3, 2020

页: [240 - 246] 页: 7

弟呕挨: 10.2174/1566524019666191023112837

价格: $65

摘要

目的：本研究旨在探讨吗啡和纳洛酮对家兔结肠组织肠蠕动及Cajal间质细胞数量的影响。 方法：30只家兔随机分为5组，每组6只，分别为生理盐水对照组（NS组），低浓度吗啡组（L组），中等浓度吗啡组（M组），高浓度吗啡组（M组）。吗啡组（H组），中等浓度的吗啡和纳洛酮与拮抗剂组（NM组）混合。在这五组中的兔子被施以硬膜外穿刺管和硬膜外背痛泵，并连续输注七天。观察粪便特性，并计算墨水推进率。通过western blot检测ICC C-kit蛋白在结肠组织中的表达水平。 结果：L，M和H组的粪便特征比NS和NM组的更为严重。此外，L，M和H组的肠道推进率低于NS和NM组。与NS和NM组相比，L，M和H组的兔子结肠中的C-kit mRNA和蛋白表达显着降低。 结论：纳洛酮阻断C-kit的mRNA和蛋白表达，改善肠道运动功能。

关键词: 吗啡，纳洛酮，硬膜外，cajal间质细胞，C-kit，mRNA。

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