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Research Article

检查miR-23a在耐热性发展中的作用

卷 20, 期 3, 2020

页: [194 - 201] 页: 8

弟呕挨: 10.2174/1566524019666191021111028

价格: $65

摘要

背景：耐热性是在暴露于非致命蛋白毒性应力后发生的，耐热性增强的一种获得状态。大量证据表明，属于热休克蛋白家族的分子伴侣分子可能是耐热状态的潜在介体。 目的：最近的证据表明，热休克蛋白HSP90，HSP70和HSP27通过在应激诱导的凋亡途径中的各个步骤进行干预，抑制了热诱导的细胞死亡。先前的研究表明，HSP70通过阻止MCL-1水平的NOXA依赖性下降（导致BAX激活和线粒体细胞色素c释放）来防止热诱导的细胞凋亡。我们还证明了表达HSP70的细胞具有增强的miR-23a水平，可以防止热诱导的NOXA水平增加并抑制细胞凋亡。 方法：使用RT-PCR和半定量RT-PCR对稳定表达对照shRNA或靶向miRNA的miR-23a稳定转染的细胞系进行定量，以确定不同的高温暴露处理对miR-23a和Noxa mRNA表达水平的影响。 结果：这项研究表明耐热诱导的预热休克治疗能够增加miR-23a的水平。此外，表达具有降低的miR-23a水平的表达靶向miR-23a的shRNA的稳定细胞克隆不能形成耐热状态，从而导致细胞凋亡。 结论：这些结果证明了miR-23a是耐热状态发展的重要因素这一新颖发现。

关键词: 耐热性，细胞凋亡，热休克蛋白，热疗，HSP70，NOXA，miR-23ª。

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