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Current Nutrition & Food Science

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ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

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Research Article

Screening of Salmonella enterica Serovars, Typhi, Typhimurium, and Enteritidis in Raw Milk and Dairy Products in South-Khorasan, Iran: Conventional versus Molecular Method

Author(s): Tayebeh Zeinali, Kobra Naseri*, Nasrin Zandi and Matin Khosravi

Volume 16, Issue 5, 2020

Page: [763 - 767] Pages: 5

DOI: 10.2174/1573401315666191010130113

Price: $65

Abstract

Background and Objective: Food-borne Salmonellosis has been reported as the second most common bacterial infection. Salmonella enterica subsp. enterica serotype enteritidis (S. Enteritidis) and S. enterica subsp. enterica serotype typhimurium (S. Typhimurium) are the most common serotypes worldwide as salmonellosis agents. Salmonella yyphi is the causative agent of typhoid fever worldwide. The purpose of the present study was to determine the contamination rate of raw milk and dairy products to Salmonella typhi, S. typhimurium and S. Enteritidis in South-Khorasan, Iran. It is very important in food safety risk assessment and human health.

Methods: A total of 260 raw milk and 181 dairy products were obtained from South-Khorasan, Iran. Dairy samples were pre-enriched in buffered peptone water and enriched in Rappaport Vassiliadis (RV). Raw milk was enriched in RV. Plating of the enriched samples was carried out on Xylose Lysine Desoxycholate (XLD) agar and Brilliant Green agar (BGA). All of the enriched samples were also tested by M-PCR for detection of S. typhi, S. typhimurium and S. Enteritidis.

Results: Among the 441 tested samples only 4 samples were contaminated with Salmonella spp. in culture method. PCR assay, didn’t find any positive sample regarding Salmonella spp. In chi-square test, the difference of two methods of isolation was significant (P< 0.05).

Conclusion: In conclusion, the results of the present study showed a good hygienic state of raw milk and dairy products. Enrichment based PCR assay is more economical than time-consuming culture method for Salmonella detection.

Keywords: Bacterial infections, dairy products, food safety, milk, molecular detection, Salmonella.

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[1]
Chen J, Zhang L, Paoli GC, Shi C, Tu S-I, Shi X. A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis. Int J Food Microbiol 2010; 137(2-3): 168-74.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2009.12.004] [PMID: 20060189]
[2]
de Freitas CG, Santana AP, da Silva PH, et al. PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat. Int J Food Microbiol 2010; 139(1-2): 15-22.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2010.02.007] [PMID: 20199820]
[3]
Akiba M, Kusumoto M, Iwata T. Rapid identification of Salmonella enterica serovars, typhimurium, choleraesuis, infantis, hadar, enteritidis, Dublin and gallinarum, by multiplex PCR. J Microbiol Methods 2011; 85(1): 9-15.
[http://dx.doi.org/10.1016/j.mimet.2011.02.002] [PMID: 21329737]
[4]
Liu B, Zhou X, Zhang L, Liu W, Dan X, Shi C, et al. Development of a novel multiplex PCR assay for the identification of Salmonella enterica typhimurium and enteritidis. Food Control 2012; 27: 87-93.
[http://dx.doi.org/10.1016/j.foodcont.2012.01.062]
[5]
Mathews S, Ngoma L, Gashe B, Mpuchane S. Assessment of pathogenic bacteria from ice cream and ice pop sold in Gaborone, Botswana. Ethno Med 2013; 7(3): 195-203.
[http://dx.doi.org/10.1080/09735070.2013.11886461]
[6]
Callaway TR, Keen JE, Edrington TS, et al. Fecal prevalence and diversity of Salmonella species in lactating dairy cattle in four states. J Dairy Sci 2005; 88(10): 3603-8.
[http://dx.doi.org/10.3168/jds.S0022-0302(05)73045-9] [PMID: 16162534]
[7]
Gwida MM, Al-Ashmawy MAM. Culture versus PCR for Salmonella Species Identification in Some Dairy Products and Dairy Handlers with Special Concern to Its Zoonotic Importance. Vet Med Int 2014; 2014, 502370
[http://dx.doi.org/10.1155/2014/502370] [PMID: 24808971]
[8]
Andrews WH, Jacobson A, Hammack T. Bacteriological Analytical Manual. Chapter 5. Salmonella. US Food and Drug Administration 2014.Available from. https://www.fda.gov/food/foodscience research/laboratorymethods/ucm070149.htm
[9]
Zeinali T, Jamshidi A, Rad M, Bassami M. A comparison analysis of Listeria monocytogenes isolates recovered from chicken carcasses and human by using RAPD PCR. Int J Clin Exp Med 2015; 8(6): 10152-7.
[PMID: 26309714]
[10]
Karshima NS, Pam VA, Bata SI, Dung PA, Paman ND. Isolation of Salmonella species from milk and locally processed milk products traded for human consumption and associated risk factors in Kanam, Plateau State, Nigeria. J Anim Prod Adv 2013; 3(3): 69-74.
[http://dx.doi.org/10.5455/japa.20130330124355]
[11]
Shaigan nia S, Rostami F, Safarpour dehkordi F, et al. Isolation and evaluation virulence factors of Salmonella typhimurium and Salmonella enteritidis in milk and dairy products. Iran J Med Microbiol 2014; 8(1): 54-61.
[12]
Rea MC, Cogan TM, Tobin S. Incidence of pathogenic bacteria in raw milk in Ireland. J Appl Bacteriol 1992; 73(4): 331-6.
[http://dx.doi.org/10.1111/j.1365-2672.1992.tb04985.x] [PMID: 1429309]
[13]
Steele ML, McNab WB, Poppe C, et al. Survey of Ontario bulk tank milk for foodborne pathogens. J Food Prot 1997; 60(11): 1341-6.
[http://dx.doi.org/10.4315/0362-028X-60.11.1341]
[14]
Tavakoli HR, Najafi A, Ahmadi A. Rapid specific and concurrent detection of Listeria, Salmonella and Escherichia coli pathogens by multiplex PCR in Iranian. J Microbiol Res (Rosemead Calif) 2010; 4(23): 2503-7.
[15]
Kawasaki S, Fratamico P. Development of the Multiplex PCR Detection kit for Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7. JPN Agric Res Quarter 2011; 45(1): 77-81.
[http://dx.doi.org/10.6090/jarq.45.77]
[16]
Omar RH, Eltinay AH. Microbial quality of camel’s raw milk in central southern region of United Arab Emirates. Emir J Food Agric 2008; 20(1): 76-83.
[http://dx.doi.org/10.9755/ejfa.v20i1.5182]
[17]
Rastegar H, Ahmadi Ashtiani HR, Afraz K, Andalibi M, et al. Detection, isolation and assessment of Salmonella entiritidis in milk by conventional culture methods and real-time PCR in Iran. Am J Res Commun 2013; 1(8): 81-97.
[18]
Ni P, Xu Q, Yin Y, Liu D, Zhang J, Wu Q, et al. Prevalence and characterization of Salmonella serovars isolated from farm products in Shanghai. Food Control 2018; 85: 269-75.
[http://dx.doi.org/10.1016/j.foodcont.2017.10.009]
[19]
Shao K. Study on types and active surveillance of foodborne Salmonella 476 Spp in Shandong province. Master's Thesis. China: Shandong University 2011.
[20]
Zavarshani M, Kownani M, Estabraghi E, Yarahmad E. Detection Salmonella spp. carriers in camel using polymerase chain reaction and cultural methods. Biological Forum -. Int J 2015; 7(1): 352-6.
[21]
Yu S, Yan L, Wu X, Li F, Wang D, Xu H. Multiplex PCR coupled with propidium monoazide for the detection of viable Cronobacter sakazakii, Bacillus cereus, and Salmonella spp. in milk and milk products. J Dairy Sci 2017; 100(10): 7874-82.
[http://dx.doi.org/10.3168/jds.2017-13110] [PMID: 28755939]
[22]
Gonzales-Barron U, Gonçalves-Tenório A, Rodrigues V, Cadavez V. Foodborne pathogens in raw milk and cheese of sheep and goat origin: a meta-analysis approach. Curr Opin Food Sci 2017; 18: 7-13.
[http://dx.doi.org/10.1016/j.cofs.2017.10.002]
[23]
Amagliani G, Petruzzelli A, Carloni E, et al. Presence of Escherichia coli O157, Salmonella spp., and Listeria monocytogenes in raw ovine milk destined for cheese production and evaluation of the equivalence between the analytical methods applied. Foodborne Pathog Dis 2016; 13(11): 626-32.
[http://dx.doi.org/10.1089/fpd.2016.2159] [PMID: 27607835]
[24]
Siala M, Barbana A, Smaoui S, et al. Screening and detecting Salmonella in different food matrices in Southern Tunisia using a combined enrichment/real-time PCR method: Correlation with Conventional Culture Method. Front Microbiol 2017; 8: 2416.
[http://dx.doi.org/10.3389/fmicb.2017.02416] [PMID: 29270157]
[25]
Alvarez J, Sota M, Vivanco AB, et al. Development of a multiplex PCR technique for detection and epidemiological typing of salmonella in human clinical samples. J Clin Microbiol 2004; 42(4): 1734-8.
[http://dx.doi.org/10.1128/JCM.42.4.1734-1738.2004] [PMID: 15071035]
[26]
Kumar S, Balakrishna K, Batra HV. Detection of Salmonella enterica serovar Typhi (S. Typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in mutiplex format. Lett Appl Microbiol 2006; 42(2): 149-54.
[http://dx.doi.org/10.1111/j.1472-765X.2005.01813.x] [PMID: 16441380]

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