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Research Article

载脂蛋白A5和人类脂肪间充质干细胞成脂调控的新见解。

卷 20, 期 2, 2020

页: [144 - 156] 页: 13

弟呕挨: 10.2174/1566524019666190927155702

价格: $65

摘要

背景：肥胖症的标志是甘油三酸酯（TG）在脂肪组织中的过度积累。载脂蛋白A5（ApoA5）已显示影响肥胖症的患病率和发病机理。但是，基本机制仍有待阐明。 方法：用600 ng / ml人重组ApoA5蛋白处理人脂肪来源的间充质干细胞（AMSC）。确定了ApoA5对细胞内TG含量和成脂相关因子表达的影响。此外，还观察到ApoA5对CIDE-C表达的影响。 结果：在脂肪形成过程中，ApoA5处理可减少脂质滴的细胞内积累和TG水平。同时，ApoA5下调了脂肪形成相关因子的表达水平，包括CCAAT增强子结合蛋白α/β（C /EBPα/β），脂肪酸合成酶（FAS）和脂肪酸结合蛋白4（FABP4）。此外，通过抑制CIDE-C表达来介导ApoA5对脂肪形成的抑制，CIDE-C表达是促进脂肪形成过程的重要因素。但是，过度表达细胞内CIDE-C可能会导致ApoA5在抑制AMSCs成脂中的功能丧失。 结论：总之，ApoA5通过至少部分下调CIDE-C表达来抑制AMSC的成脂过程。本研究提供了新颖的机制，通过该机制，ApoA5可通过人类的AMSC预防肥胖。

关键词: 载脂蛋白A5，CIDE-C，脂肪来源的间充质干细胞，脂肪形成，抑制，AMSC

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