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Research Article

硬脂酸对猪肠道上皮细胞增殖，分化，凋亡和自噬的影响

卷 20, 期 2, 2020

页: [157 - 166] 页: 10

弟呕挨: 10.2174/1566524019666190917144127

价格: $65

摘要

背景：硬脂酸（SA）是一种由18个碳原子组成的饱和长链脂肪酸，广泛存在于饲料成分中，例如玉米，大豆和小麦。然而，SA在肠上皮细胞更新中的作用仍不清楚。 方法和结果：在本研究中，我们发现0.01-0.1 mM SA促进IPEC-J2细胞分化，并且不影响IPEC-J2细胞活力。另外，结果表明，在高浓度下，SA以时间和剂量依赖性方式抑制了IPEC-J2细胞的活力。流式细胞仪和蛋白质印迹分析表明SA诱导细胞凋亡，自噬和内质网应激。另外，用SA攻击后，甘油三酸酯的量显着增加。而且，由SA诱导的细胞活力的降低可以被4-PBA（一种ER应激的抑制剂）减轻。 结论：总之，SA在0.01-0.1 mM的速度下加速了IPEC-J2细胞的分化。此外，SA通过引起ER应激而诱导IPEC-J2细胞凋亡和自噬。

关键词: 硬脂酸，IPEC-J2细胞，内质网应激，凋亡，自噬，甘油三酸酯。

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