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Research Article

梗阻性黄疸及肠内外胆管引流后小鼠肠上皮Paneth细胞的功能变化

卷 19, 期 10, 2019

页: [746 - 757] 页: 12

弟呕挨: 10.2174/1566524019666190820141331

价格: $65

摘要

目的：探讨梗阻性黄疸（OJ）小鼠及胆内引流（ID）和胆外引流（ED）小鼠肠上皮中Paneth细胞的功能变化。 方法：实验分为两个阶段。第一阶段：将小鼠随机分为两组：（I）假手术（SH）； （II）OJ。在手术前和手术后的第1、3、5和7天处死小鼠以收集标本。第二阶段：将小鼠随机分为四组：（I）SH； （II）OJ； （III）OJ和ED； （IV）OJ和ID。他们在第5天因胆道引流手术而再次手术。第10天收集标本。 结果：OJ组随时间的变化溶菌酶和cryptdin-4的表达先升高后降低，随着OJ时间的延长，Paneth细胞数逐渐减少（p <0.05）。小鼠二次手术缓解OJ后，ID组的Paneth细胞数量以及溶菌酶和cryptdin-4的表达均明显高于ED组（p <0.05）。 结论：OJ可引起小鼠肠道Paneth细胞功能异常。在恢复Paneth细胞功能方面，ID比ED更重要。这可能是使ID优于ED的机制之一。

关键词: 阻塞性黄疸，Paneth细胞，隐蛋白，溶菌酶，肠粘膜屏障，细菌易位。

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