Abstract
We previously reported that surface-linked liposomal antigen induced IgEselective unresponsiveness. The results were consistent even when different coupling procedures for antigen with liposomes, or for liposomes with different lipid components, were employed. During the course of an investigation intended to clarify the mechanism of IgE-selective unresponsiveness induced by surface-coupled liposomal antigens, we discovered an alternative approach to regulate the production of IgE, one that is independent of the activity of T-cells. Immunization of mice with OVA-liposome conjugates induced IgE- selective unresponsiveness without apparent Th1 polarization. Neither interleukin-12 (IL-12), IL-10, nor CD8+ T-cells participated in the regulation. Further, CD4+ T-cells of mice immunized with OVA-liposome were capable of inducing antigen-specific IgE synthesis in athymic nude mice immunized with alum-adsorbed OVA. On the other hand, immunization of the recipient mice with OVAliposome did not induce anti-OVA IgE production, even when CD4+ T-cells of mice immunized with alumadsorbed OVA were transferred. In the secondary immune response, OVA-liposome enhanced anti-OVA IgG antibody production but not the ongoing IgE production, suggesting that the IgE-selective unresponsiveness induced by the liposomal antigen involved direct effects on IgE but not IgG switching in vivo. These results suggest the role of an alternative mechanism, one not involving T-cells, in the regulation of IgE synthesis, and raise the possibility that surface-linked liposomal antigen is potentially applicable for the development of a novel vaccine that induces the least IgE synthesis. Moreover, given the relatively low allergic response to and increased antigenicity of the allergen, this form of antigen preparation would be applicable to allergen immunotherapy.
Current Drug Targets - Immune, Endocrine & Metabolic Disorders
Title: Surface-Linked Liposomal Antigen Induces IgE Selective Unresponsiveness in a T-Cell Independent Fashion
Volume: 3 Issue: 2
Author(s): Tetsuya Uchida
Affiliation:
Keywords: liposome, Ige, vaccine, allergy
Abstract: We previously reported that surface-linked liposomal antigen induced IgEselective unresponsiveness. The results were consistent even when different coupling procedures for antigen with liposomes, or for liposomes with different lipid components, were employed. During the course of an investigation intended to clarify the mechanism of IgE-selective unresponsiveness induced by surface-coupled liposomal antigens, we discovered an alternative approach to regulate the production of IgE, one that is independent of the activity of T-cells. Immunization of mice with OVA-liposome conjugates induced IgE- selective unresponsiveness without apparent Th1 polarization. Neither interleukin-12 (IL-12), IL-10, nor CD8+ T-cells participated in the regulation. Further, CD4+ T-cells of mice immunized with OVA-liposome were capable of inducing antigen-specific IgE synthesis in athymic nude mice immunized with alum-adsorbed OVA. On the other hand, immunization of the recipient mice with OVAliposome did not induce anti-OVA IgE production, even when CD4+ T-cells of mice immunized with alumadsorbed OVA were transferred. In the secondary immune response, OVA-liposome enhanced anti-OVA IgG antibody production but not the ongoing IgE production, suggesting that the IgE-selective unresponsiveness induced by the liposomal antigen involved direct effects on IgE but not IgG switching in vivo. These results suggest the role of an alternative mechanism, one not involving T-cells, in the regulation of IgE synthesis, and raise the possibility that surface-linked liposomal antigen is potentially applicable for the development of a novel vaccine that induces the least IgE synthesis. Moreover, given the relatively low allergic response to and increased antigenicity of the allergen, this form of antigen preparation would be applicable to allergen immunotherapy.
Export Options
About this article
Cite this article as:
Uchida Tetsuya, Surface-Linked Liposomal Antigen Induces IgE Selective Unresponsiveness in a T-Cell Independent Fashion, Current Drug Targets - Immune, Endocrine & Metabolic Disorders 2003; 3 (2) . https://dx.doi.org/10.2174/1568005310303020119
DOI https://dx.doi.org/10.2174/1568005310303020119 |
Print ISSN 1568-0088 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5917 |
Related Articles
-
Hepatitis C Virus Infection: Immune Responsiveness and Interferon-a Treatment
Current Pharmaceutical Design Pathology of Multiple Sclerosis
CNS & Neurological Disorders - Drug Targets Treating Older HIV-1-infected Subjects With Cobicistat-boosted Darunavir in a 48-week Phase 3 Trial
Reviews on Recent Clinical Trials Advancement in Shampoo (A Dermal Care Product): Preparation Methods, Patents and Commercial Utility
Recent Patents on Inflammation & Allergy Drug Discovery Human Recombinant Relaxin (Serelaxin) as Anti-fibrotic Agent: Pharmacology, Limitations and Actual Perspectives
Current Molecular Medicine Targeting Enzymes with Phosphonate-Based Inhibitors: Mimics of Tetrahedral Transition States and Stable Isosteric Analogues of Phosphates
Current Enzyme Inhibition Subject Index To Volume 2
Current Women`s Health Reviews Lung Volume Reduction in Patients with COPD: Physiological and Clinical Implications
Current Respiratory Medicine Reviews Disruption of Circadian Rhythms and Sleep in Critical Illness and its Impact on the Development of Delirium
Current Pharmaceutical Design Glycaemic Control in Cardiac Surgery Patients: a Double-Edged Sword
Current Vascular Pharmacology Are Antipsychotics Useful in the Treatment of Anorexia Nervosa? A Review of the Literature
Current Psychopharmacology Immunotherapy-Based Strategies for the Treatment of Autoimmune Diabetes: Searching for the Cure
Current Pharmaceutical Design History and Milestones of Mouse Models of Autoimmune Diseases
Current Pharmaceutical Design COX-2 in Brain and Spinal Cord - Implications for Therapeutic Use.
Current Medicinal Chemistry Application of Radiolabeled Antibodies in Targeting Therapy of Breast Cancer
Current Molecular Imaging (Discontinued) Vaccines and Vaccine Strategies Against HIV
Current Drug Targets STAT3: A Molecular Target for Cancer Whose Time Has Come
Current Signal Transduction Therapy Mas Receptor Agonists as Novel Antihypertensive Agents
Current Hypertension Reviews AMPK - Activated Protein Kinase and its Role in Energy Metabolism of the Heart
Current Cardiology Reviews Astroglial Connexins as a Therapeutic Target for Alzheimer's Disease
Current Pharmaceutical Design