New Emergent Nanotechnologies in Medical and Biochemical Applications:Advanced Fluorescence Protein-Based Nanosensors

Title: New Emergent Nanotechnologies in Medical and Biochemical Applications:Advanced Fluorescence Protein-Based Nanosensors

Volume: 1 Issue: 1

Author(s): Maria Staiano, Marcella de Champdore, Stefano Borini, Andrea M. Rossi, Mose Rossi and Sabato D'Auria

Affiliation:

Keywords: Porous silicon, proteins, diabetes, fluorescence, biosensors

Abstract: In this review we explore the advantages deriving from the use of either enzymes or binding proteins to develop non-consuming substrate fluorescence biosensors. We report on a novel approach to address the consumption of substrate by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins, which through still able to bind the ligand yet cannot transform it into product. We also report recent studies in which fluorescence labelling of a naturally thermostable binding protein by a fluorescent probe allows a quantitative monitoring of glucose. Finally, we will illustrate a novel methodology based on the utilization of porous silicon chips that allows a nanotechnological approach to the realization of protein arrays for analyses of high medical and biochemical interest.

Cite this article as:

Staiano Maria, de Champdore Marcella, Borini Stefano, Rossi M. Andrea, Rossi Mose and D'Auria Sabato, New Emergent Nanotechnologies in Medical and Biochemical Applications:Advanced Fluorescence Protein-Based Nanosensors, Current Chemical Biology 2007; 1 (1) . https://dx.doi.org/10.2174/2212796810701010003