Abstract
Peptidylarginine deiminase IV (PAD4) catalyzes the conversion of an Arg residue to a citrulline residue in various proteins. In particular, citrullination of histone subunits, such as H2A and H3, by PAD4 is thought to be related to rheumatoid arthritis. However, the details of the citrullination mechanism of histone H2A and H3 are not yet well known. Moreover, the effects of N-terminal acetylation on histone subunits with respect to PAD4 recognition have not yet been studied. To further study the mechanism of PAD4 recognition of histone H2A and H3 subunits, a series of the N-terminal peptides was chemically synthesized and the citrullination sites were identified using MALDI-TOF/MS. N-terminal acetylation of histone H2A was not significant with respect to PAD4 recognition in vitro, but the acetylation of H3 peptide had a significant effect on PAD4 recognition in vitro, resulting in predominant citrullination at the Arg2 residue.
Keywords: Histone, Acetylation, Peptidylarginine deiminase, Citrullination, Rheumatoid arthritis
Protein & Peptide Letters
Title: Recognition of the N-Terminal Histone H2A and H3 Peptides by Peptidylarginine Deiminase IV
Volume: 16 Issue: 9
Author(s): Masatoshi Saiki, Mayumi Watase, Hironori Matsubayashi and Yuji Hidaka
Affiliation:
Keywords: Histone, Acetylation, Peptidylarginine deiminase, Citrullination, Rheumatoid arthritis
Abstract: Peptidylarginine deiminase IV (PAD4) catalyzes the conversion of an Arg residue to a citrulline residue in various proteins. In particular, citrullination of histone subunits, such as H2A and H3, by PAD4 is thought to be related to rheumatoid arthritis. However, the details of the citrullination mechanism of histone H2A and H3 are not yet well known. Moreover, the effects of N-terminal acetylation on histone subunits with respect to PAD4 recognition have not yet been studied. To further study the mechanism of PAD4 recognition of histone H2A and H3 subunits, a series of the N-terminal peptides was chemically synthesized and the citrullination sites were identified using MALDI-TOF/MS. N-terminal acetylation of histone H2A was not significant with respect to PAD4 recognition in vitro, but the acetylation of H3 peptide had a significant effect on PAD4 recognition in vitro, resulting in predominant citrullination at the Arg2 residue.
Export Options
About this article
Cite this article as:
Saiki Masatoshi, Watase Mayumi, Matsubayashi Hironori and Hidaka Yuji, Recognition of the N-Terminal Histone H2A and H3 Peptides by Peptidylarginine Deiminase IV, Protein & Peptide Letters 2009; 16 (9) . https://dx.doi.org/10.2174/092986609789055449
DOI https://dx.doi.org/10.2174/092986609789055449 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Antimalarial Drugs and their Useful Therapeutic Lives: Rational Drug Design Lessons from Pleiotropic Action of Quinolines and Artemisinins
Current Drug Discovery Technologies Immune Aging and Autoimmune Diseases in Children
Current Immunology Reviews (Discontinued) Targeting Heat Shock Protein 90 for Malaria
Mini-Reviews in Medicinal Chemistry Pyridinylimidazole Based p38 MAP Kinase Inhibitors
Current Topics in Medicinal Chemistry NF-κB in Anti-Inflammatory Activity of Probiotics: An Update
Current Immunology Reviews (Discontinued) Polymorphism Gln27Glu of β2 Adrenergic Receptors in Patients with Ischaemic Cardiomyopathy
Current Vascular Pharmacology TLRs Play Crucial Roles in Regulating RA Synoviocyte
Endocrine, Metabolic & Immune Disorders - Drug Targets MicroRNAs-based Therapy: A Novel and Promising Strategy for Cancer Treatment
MicroRNA IL-22 and Its Receptors, New Players in the Inflammatory Network
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Diclofenac 1,3,4-Oxadiazole Derivatives; Biology-Oriented Drug Synthesis (BIODS) in Search of Better Non-Steroidal, Non-Acid Antiinflammatory Agents
Medicinal Chemistry Clinical Analysis Methods of Voice Disorders
Current Bioinformatics Antisense Strategies
Current Molecular Medicine Genetic Insights into Sepsis: What have we Learned and How will it Help?
Current Pharmaceutical Design Development and Validation of a Patient Reported Experience Measure (PREM) for Patients with Rheumatoid Arthritis (RA) and other Rheumatic Conditions
Current Rheumatology Reviews Engineering of Therapeutic Proteins Production in Escherichia coli
Current Pharmaceutical Biotechnology Targeting Receptor Tyrosine Kinases Using Monoclonal Antibodies: The Most Specific Tools for Targeted-Based Cancer Therapy
Current Drug Targets Anti-cytokines and Cytokines in the Treatment of Rheumatoid Arthritis
Current Pharmaceutical Design Systemic Sclerosis-Related Interstitial Lung Disease
Current Respiratory Medicine Reviews Paracrine Provision of Lipids in the Immune System
Current Immunology Reviews (Discontinued) Cytokine Status of Serum in Ovarian Cancer Patients with Different Tumor Neoadjuvant Chemotherapy Response
Anti-Cancer Agents in Medicinal Chemistry