Abstract
Hepatopancreas of king crab Paralithodes camtschatica produces a metalloprotease, which belongs to the astacin family, as cDNA cloning and sequencing showed. The metalloprotease has been purified chromatographically to apparent homogeneity. The purification factor was 16 and activity recovery was 20%. pH and temperature optimum have been determined. In its properties (molecular weight, pI, metal content) the metalloprotease is close to crayfish astacin. However, analysis of the enzyme sequences revealed differences, which account for differences in substrate specificities and imply a different activation mechanism.
Keywords: Metalloprotease, astacin family, affinity chromatography, Paralithodes camtschatica
Protein & Peptide Letters
Title: cDNA Cloning, Purification and Properties of Paralithodes camtschatica Metalloprotease
Volume: 13 Issue: 6
Author(s): Svetlana A. Semenova, Galina N. Rudenskaya, Denis V. Rebrikov and Vyacheslav A. Isaev
Affiliation:
Keywords: Metalloprotease, astacin family, affinity chromatography, Paralithodes camtschatica
Abstract: Hepatopancreas of king crab Paralithodes camtschatica produces a metalloprotease, which belongs to the astacin family, as cDNA cloning and sequencing showed. The metalloprotease has been purified chromatographically to apparent homogeneity. The purification factor was 16 and activity recovery was 20%. pH and temperature optimum have been determined. In its properties (molecular weight, pI, metal content) the metalloprotease is close to crayfish astacin. However, analysis of the enzyme sequences revealed differences, which account for differences in substrate specificities and imply a different activation mechanism.
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Cite this article as:
Semenova A. Svetlana, Rudenskaya N. Galina, Rebrikov V. Denis and Isaev A. Vyacheslav, cDNA Cloning, Purification and Properties of Paralithodes camtschatica Metalloprotease, Protein & Peptide Letters 2006; 13 (6) . https://dx.doi.org/10.2174/092986606777145887
DOI https://dx.doi.org/10.2174/092986606777145887 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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