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Current Proteomics

Editor-in-Chief

ISSN (Print): 1570-1646
ISSN (Online): 1875-6247

Research Article

iTRAQ-Based Proteomic Analysis of the Rat Pancreas Following Gastric Bypass Surgery

Author(s): Zhuolin Xie, Lu Bai, Xiang Gao, Liyong Zhu, Shaihong Zhu, Weizheng Li* and Pengzhou Li*

Volume 18, Issue 4, 2021

Published on: 16 November, 2020

Page: [480 - 490] Pages: 11

DOI: 10.2174/1570164617999201116153530

Price: $65

Abstract

Aim: There are many people with type 2 Diabetes Mellitus (T2DM) worldwide. Rouxen- Y Gastric Bypass (RYGB) is an effective surgery for treating T2DM with beneficial effects on β cell metabolism. However, the mechanism of how RYGB affects the pancreas, is not clear. We focused on metabolic changes in the pancreas of rats following RYGBand to investigate complex postoperative pancreatic metabolic reprogramming and to understand how RYGB improves islet function.

Methods: We performed RYGBonstreptozotocin-induced male T2DM rats. Then we measured indicators such as weight, fasting GLU, etc. After 10 weeks, pancreatic tissues were removed to identify and quantify differentially expressed proteins. Furthermore, functional analysis of these proteins and their associated pathways was conducted by bioinformatics methods.

Results: After surgery, 451 differentially expressed proteins associated with the mechanism ofRYGB were identified. Protein levels of regenerating islet-derived protein 3 (Reg3A), cell division cycle-associated protein 2 (CDCA2), and ADP-ribosylation factor 1 (Arf1) varied greatly, and Arf1 may be a point target in diabetes.

Conclusion: This research shows that RYGB could treat T2DM through changes in the pancreatic proteins. It will give some new insight into the molecular mechanism behind the effect of RYGB.

Keywords: T2DM, RYGB, proteomics, pancreatic protein, reg3A, CDCA2, Arf1.

Graphical Abstract


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