Abstract
A determination method for amino acids in mouse plasma was developed by HPLC with fluorescence detection. The developed methodology is based on a pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) carried out in the sample injection loop. The optimized derivatization time and temperature were 10 min and 60 °C, respectively. The separation of 21 amino acid derivatives was achieved on a monolithic silica column with gradient elution. The amino acid derivatives were detected with excitation and emission at 470 nm and 530 nm, respectively. The lower limit of detection for the individual amino acids was 1.52 to 47.0 fmol. The calibration curves for all the amino acids were linear over the tested range of 0.03 to 30 pmol. The validation data showed that the assay is sensitive, selective and reproducible for determination of amino acids. The analytical method was successfully applied to determine the amino acids in a mouse plasma sample.
Keywords: Mouse, plasma, NBD-F, fluorescence.
Graphical Abstract
Current Analytical Chemistry
Title:Amino Acids Analysis Using in Loop Derivatization with 4-fluoro-7-nitro- 2,1,3-benzoxadiazole
Volume: 10 Issue: 3
Author(s): Makoto Tsunoda, Yanting Song and Takashi Funatsu
Affiliation:
Keywords: Mouse, plasma, NBD-F, fluorescence.
Abstract: A determination method for amino acids in mouse plasma was developed by HPLC with fluorescence detection. The developed methodology is based on a pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) carried out in the sample injection loop. The optimized derivatization time and temperature were 10 min and 60 °C, respectively. The separation of 21 amino acid derivatives was achieved on a monolithic silica column with gradient elution. The amino acid derivatives were detected with excitation and emission at 470 nm and 530 nm, respectively. The lower limit of detection for the individual amino acids was 1.52 to 47.0 fmol. The calibration curves for all the amino acids were linear over the tested range of 0.03 to 30 pmol. The validation data showed that the assay is sensitive, selective and reproducible for determination of amino acids. The analytical method was successfully applied to determine the amino acids in a mouse plasma sample.
Export Options
About this article
Cite this article as:
Tsunoda Makoto, Song Yanting and Funatsu Takashi, Amino Acids Analysis Using in Loop Derivatization with 4-fluoro-7-nitro- 2,1,3-benzoxadiazole, Current Analytical Chemistry 2014; 10 (3) . https://dx.doi.org/10.2174/1573411010999131219102447
DOI https://dx.doi.org/10.2174/1573411010999131219102447 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Alert Cell Strategy: Mechanisms of Inflammatory Response and Organ Protection
Current Pharmaceutical Design What Metabolic Syndrome Contributes to Brain Outcomes in African American & Caucasian Cohorts
Current Alzheimer Research Studying the Human Gut Microbiota in the Trans-Omics Era - Focus on Metagenomics and Metabonomics
Current Pharmaceutical Design Keratin 18, Apoptosis, and Liver Disease in Children
Current Pediatric Reviews Tumor-Receptor Imaging in Breast Cancer: A Tool for Patient Selection and Response Monitoring
Current Molecular Medicine Antioxidant Activity of Galantamine and Some of its Derivatives
Current Medicinal Chemistry The Physiological Characteristics and Transcytosis Mechanisms of the Blood-Brain Barrier (BBB)
Current Pharmaceutical Biotechnology Effects of School-Based Physical Activity Interventions on Cognition and Academic Achievement: A Systematic Review
CNS & Neurological Disorders - Drug Targets Nitric Oxide Related Therapeutic Phenomenon: A Challenging Task
Current Pharmaceutical Design Molecular Basis of Cardioprotection by Erythropoietin
Current Molecular Pharmacology Ceramic Nanoparticles: Fabrication Methods and Applications in Drug Delivery
Current Pharmaceutical Design The Role of Dairy Products in Healthy Weight and Body Composition in Children and Adolescents
Current Nutrition & Food Science Hypothalamic Neuropeptide Systems as Targets for Potential Anti-Obesity Drugs
Mini-Reviews in Medicinal Chemistry Outfielders Playing in the Infield: Functions of Aging-Associated "Nuclear" Proteins in the Mitochondria
Current Molecular Medicine Monoclonal Antibodies in Cancer Therapy
Current Medicinal Chemistry - Anti-Cancer Agents Antiglycation Activity of Triazole Schiff’s Bases Against Fructosemediated Glycation: In Vitro and In Silico Study
Medicinal Chemistry Non-Vitamin K Oral Anticoagulants (NOACs) A Review of Clinical Management and Laboratory Issues
Current Vascular Pharmacology Effect of Nitric Oxide Synthase Inhibition on Body Temperature Control During Endotoxemic Shock
Current Enzyme Inhibition Avocado (Persea americana) Seed as a Source of Bioactive Phytochemicals
Current Pharmaceutical Design IN MEMORIAM
Endocrine, Metabolic & Immune Disorders - Drug Targets