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Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Review Article

Current Advances on the Extraction and Identification of Bioactive Components of Sage (Salvia spp.)

Author(s): Efthymios Poulios*, Constantinos Giaginis and Georgios K. Vasios*

Volume 20, Issue 10, 2019

Page: [845 - 857] Pages: 13

DOI: 10.2174/1389201020666190722130440

Price: $65

Abstract

Common sage (Salvia spp., with the most common species Salvia officinalis L., Lamiaceae) is an important medicinal and aromatic plant, with antioxidant, antimicrobial, anti-inflammatory and anticancer properties. The polyphenolic compounds are mainly responsible for its diverse biological activity. Many different methods for extraction and identification of sage bioactive compounds, using various solvents, have been developed in recent years. The total phenolic content is usually measured and identified by various technical methodologies with different sensitivity and specificity. In this aspect, the present review is aimed to critically summarize and discuss various technical approaches for the extraction and identification of sage bioactive components in order to point out the more appropriate approaches of them.

Keywords: Salvia species, sage, extraction techniques, antioxidant activity, phenolic compounds, Folin-Ciocalteu assay, DPPH assay.

Graphical Abstract

[1]
Ghorbani, A.; Esmaeilizadeh, M. Pharmacological properties of Salvia officinalis and its components. J. Tradit. Complement. Med., 2017, 7(4), 433-440.
[http://dx.doi.org/10.1016/j.jtcme.2016.12.014] [PMID: 29034191]
[2]
Guerriero, G.; Berni, R.; Muñoz-Sanchez, J.A.; Apone, F.; Abdel-Salam, E.M.; Qahtan, A.A.; Alatar, A.A.; Cantini, C.; Cai, G.; Hausman, J.F.; Siddiqui, K.S.; Hernández-Sotomayor, S.M.T.; Faisal, M. Production of plant secondary metabolites: Examples, tips and suggestions for biotechnologists. Genes (Basel), 2018, 9(6), 309.
[http://dx.doi.org/10.3390/genes9060309] [PMID: 29925808]
[3]
Tungmunnithum, D.; Thongboonyou, A.; Pholboon, A.; Yangsabai, A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines (Basel), 2018, 5(3), 93.
[http://dx.doi.org/10.3390/medicines5030093] [PMID: 30149600]
[4]
Berdowska, I.; Zieliński, B.; Fecka, I.; Kulbacka, J.; Saczko, J.; Gamian, A. Cytotoxic impact of phenolics from Lamiaceae species on human breast cancer cells. Food Chem., 2013, 141(2), 1313-1321.
[http://dx.doi.org/10.1016/j.foodchem.2013.03.090] [PMID: 23790919]
[5]
Marchev, A.; Haas, C.; Schulz, S.; Georgiev, V.; Steingroewer, J.; Bley, T.; Pavlov, A. Sage in vitro cultures: A promising tool for the production of bioactive terpenes and phenolic substances. Biotechnol. Lett., 2014, 36(2), 211-221.
[http://dx.doi.org/10.1007/s10529-013-1350-z] [PMID: 24129950]
[6]
Areias, F.; Valentão, P.; Andrade, P.B.; Ferreres, F.; Seabra, R.M. Flavonoids and phenolic acids of sage: influence of some agricultural factors. J. Agric. Food Chem., 2000, 48(12), 6081-6084.
[http://dx.doi.org/10.1021/jf000440+] [PMID: 11141272]
[7]
Santos-Gomes, P.C.; Seabra, R.M.; Andrade, P.B.; Fernandes-Ferreira, M. Determination of phenolic antioxidant compounds produced by calli and cell suspensions of sage (Salvia officinalis L.). J. Plant Physiol., 2003, 160(9), 1025-1032.
[http://dx.doi.org/10.1078/0176-1617-00831] [PMID: 14593803]
[8]
Schwarz, K.; Ternes, W. Antioxidative constituents of Rosmarinus officinalis and Salvia officinalis. II. Isolation of carnosic acid and formation of other phenolic diterpenes. Z. Lebensm. Unters. Forsch., 1992, 195(2), 99-103.
[http://dx.doi.org/10.1007/BF01201766] [PMID: 1529648]
[9]
Gericke, S.; Lübken, T.; Wolf, D.; Kaiser, M.; Hannig, C.; Speer, K. Identification of new compounds from sage flowers (Salvia officinalis L.) as markers for quality control and the influence of the manufacturing technology on the chemical composition and antibacterial activity of sage flower extracts. J. Agric. Food Chem., 2018, 66(8), 1843-1853.
[http://dx.doi.org/10.1021/acs.jafc.8b00581] [PMID: 29448760]
[10]
Živković, J.; Ristić, M.; Kschonsek, J.; Westphal, A.; Mihailović, M.; Filipović, V.; Böhm, V. Comparison of chemical profile and antioxidant capacity of seeds and oils from Salvia sclarea and Salvia officinalis. Chem. Biodivers., 2017, 14(12)e1700344
[http://dx.doi.org/10.1002/cbdv.201700344] [PMID: 28865183]
[11]
Shi, M.; Huang, F.; Deng, C.; Wang, Y.; Kai, G. Bioactivities, biosynthesis and biotechnological production of phenolic acids in Salvia miltiorrhiza. Crit. Rev. Food Sci. Nutr., 2018, 10, 1-12.
[http://dx.doi.org/10.1080/10408398.2018.1474170] [PMID: 29746788]
[12]
Lu, Y.; Foo, L.Y. Flavonoid and phenolic glycosides from Salvia officinalis. Phytochemistry, 2000, 55(3), 263-267.
[http://dx.doi.org/10.1016/S0031-9422(00)00309-5] [PMID: 11142853]
[13]
Pavić, V.; Jakovljević, M.; Molnar, M.; Jokić, S. Extraction of carnosic acid and carnosol from sage (Salvia officinalis L.) leaves by supercritical fluid extraction and their antioxidant and antibacterial activity. Plants (Basel), 2019, 8(1), 16.
[http://dx.doi.org/10.3390/plants8010016] [PMID: 30634542]
[14]
Bakota, E.L.; Winkler-Moser, J.K.; Berhow, M.A.; Eller, F.J.; Vaughn, S.F. Antioxidant activity and sensory evaluation of a rosmarinic acid-enriched extract of Salvia officinalis. J. Food Sci., 2015, 80(4), C711-C717.
[http://dx.doi.org/10.1111/1750-3841.12837] [PMID: 25808312]
[15]
Glisic, S.B.; Ristic, M.; Skala, D.U. The combined extraction of sage (Salvia officinalis L.): Ultrasound followed by supercritical CO2 extraction. Ultrason. Sonochem., 2011, 18(1), 318-326.
[http://dx.doi.org/10.1016/j.ultsonch.2010.06.011] [PMID: 20634117]
[16]
Lugemwa, F.N. Extraction of betulin, trimyristin, eugenol and carnosic acid using water-organic solvent mixtures. Molecules, 2012, 17(8), 9274-9282.
[http://dx.doi.org/10.3390/molecules17089274] [PMID: 22864237]
[17]
Fatma Ebru, K.; Ayse, A.; Caglar, K. Extraction and HPLC analysis of sage (Salvia officinalis) plant. Nat. Prod. Chem. Res., 2018, 5(8), 298.
[http://dx.doi.org/10.4172/2329-6836.1000298]
[18]
Alcântara, M.A.; de Lima Brito Polari, I.; de Albuquerque Meireles, B.R.L.; de Lima, A.E.A.; da Silva, Junior J.C.; de Andrade Vieira, É.; Dos Santos, N.A.; de Magalhães Cordeiro, A.M.T. Effect of the solvent composition on the profile of phenolic compounds extracted from chia seeds. Food Chem., 2019, 275, 489-496.
[http://dx.doi.org/10.1016/j.foodchem.2018.09.133] [PMID: 30724224]
[19]
Kozłowska, M.; Laudy, A.E.; Przybył, J.; Ziarno, M.; Majewska, E. Chemical composition and antibacterial activity of some medicinal plants from Lamiaceace family. Acta Pol. Pharm., 2015, 72(4), 757-767.
[PMID: 26647633]
[20]
Walch, S.G.; Lachenmeier, D.W.; Kuballa, T.; Stühlinger, W.; Monakhova, Y.B. Holistic control of herbal teas and tinctures based on sage (Salvia officinalis L.) for compounds with beneficial and adverse effects using NMR spectroscopy. Anal. Chem. Insights, 2012, 7, 1-12.
[http://dx.doi.org/10.4137/ACI.S8946] [PMID: 22493561]
[21]
Koysu, P.; Genc, N.; Elmastas, M.; Aksit, H.; Erenler, R. Isolation, identification of secondary metabolites from Salvia absconditi flora and evaluation of their antioxidative properties. Nat. Prod. Res., 2018, 0, 1-4.
[http://dx.doi.org/10.1080/14786419.2018.1488700] [PMID: 30445824]
[22]
Zengin, G.; Senkardes, I.; Mollica, A.; Picot-Allain, C.M.N.; Bulut, G.; Dogan, A.; Mahomoodally, M.F. New insights into the in vitro biological effects, in silico docking and chemical profile of clary sage - Salvia sclarea L. Comput. Biol. Chem., 2018, 75, 111-119.
[http://dx.doi.org/10.1016/j.compbiolchem.2018.05.005] [PMID: 29772456]
[23]
Srivedavyasasri, R.; Hayes, T.; Ross, S.A. Phytochemical and biological evaluation of Salvia apiana. Nat. Prod. Res., 2017, 31(17), 2058-2061.
[http://dx.doi.org/10.1080/14786419.2016.1269096] [PMID: 28025900]
[24]
Cvetkovikj, I.; Stefkov, G.; Acevska, J.; Stanoeva, J.P.; Karapandzova, M.; Stefova, M.; Dimitrovska, A.; Kulevanova, S. Polyphenolic characterization and chromatographic methods for fast assessment of culinary Salvia species from South East Europe. J. Chromatogr. A, 2013, 1282, 38-45.
[http://dx.doi.org/10.1016/j.chroma.2012.12.068] [PMID: 23415138]
[25]
Torun, M.; Dincer, C.; Topuz, A.; Sahin-Nadeem, H.; Ozdemir, F. Aqueous extraction kinetics of soluble solids, phenolics and flavonoids from sage (Salvia fruticosa Miller) leaves. J. Food Sci. Technol., 2015, 52(5), 2797-2805.
[http://dx.doi.org/10.1007/s13197-014-1308-8] [PMID: 25892777]
[26]
Grzegorczyk-Karolak, I.; Kiss, A.K. Determination of the phenolic profile and antioxidant properties of Salvia viridis L. shoots: A comparison of aqueous and hydroethanolic extracts. Molecules, 2018, 23(6), 1468.
[http://dx.doi.org/10.3390/molecules23061468] [PMID: 29914194]
[27]
Omar, J.; Olivares, M.; Alonso, I.; Vallejo, A.; Aizpurua-Olaizola, O.; Etxebarria, N. Quantitative analysis of bioactive compounds from aromatic plants by means of dynamic headspace extraction and multiple headspace extraction-gas chromatography-mass spectrometry. J. Food Sci., 2016, 81(4), C867-C873.
[http://dx.doi.org/10.1111/1750-3841.13257] [PMID: 26925555]
[28]
Sajewicz, M.; Staszek, D.; Wojtal, Ł.; Kowalska, T.; Hajnos, M.Ł.; Waksmundzka-Hajnos, M. Binary HPLC-diode array detector and HPLC-evaporative light-scattering detector fingerprints of methanol extracts from the selected sage (Salvia) species. J. AOAC Int., 2011, 94(1), 71-76.
[PMID: 21391482]
[29]
da Silva, S.B.; Oliveira, A.; Ferreira, D.; Sarmento, B.; Pintado, M. Development and validation method for simultaneous quantification of phenolic compounds in natural extracts and nanosystems. Phytochem. Anal., 2013, 24(6), 638-644.
[http://dx.doi.org/10.1002/pca.2446] [PMID: 23716364]
[30]
Zimmermann, B.F.; Walch, S.G.; Tinzoh, L.N.; Stühlinger, W.; Lachenmeier, D.W. Rapid UHPLC determination of polyphenols in aqueous infusions of Salvia officinalis L. (sage tea). J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2011, 879(24), 2459-2464.
[http://dx.doi.org/10.1016/j.jchromb.2011.06.038] [PMID: 21783434]
[31]
Zheng, W.; Wang, S.Y. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem., 2001, 49(11), 5165-5170.
[http://dx.doi.org/10.1021/jf010697n] [PMID: 11714298]
[32]
Kim, T.Y.; Cho, J.Y.; Lee, Y.G.; Jeong, H.Y.; Lee, H.J.; Moon, J.H. Phenolics and eudesmanolide from aged common sage exudate with sugar. Food Sci. Biotechnol., 2017, 26(6), 1491-1500.
[http://dx.doi.org/10.1007/s10068-017-0234-8] [PMID: 30263686]
[33]
Hossain, M.B.; Rai, D.K.; Brunton, N.P.; Martin-Diana, A.B.; Barry-Ryan, C. Characterization of phenolic composition in Lamiaceae spices by LC-ESI-MS/MS. J. Agric. Food Chem., 2010, 58(19), 10576-10581.
[http://dx.doi.org/10.1021/jf102042g] [PMID: 20825192]
[34]
Rzepa, J.; Wojtal, L.; Staszek, D.; Grygierczyk, G.; Labe, K.; Hajnos, M.; Kowalska, T.; Waksmundzka-Hajnos, M. Fingerprint of selected Salvia species by HS-GC-MS analysis of their volatile fraction. J. Chromatogr. Sci., 2009, 47(7), 575-580.
[http://dx.doi.org/10.1093/chromsci/47.7.575] [PMID: 19772730]
[35]
Lamien-Meda, A.; Nell, M.; Lohwasser, U.; Börner, A.; Franz, C.; Novak, J. Investigation of antioxidant and rosmarinic acid variation in the sage collection of the genebank in Gatersleben. J. Agric. Food Chem., 2010, 58(6), 3813-3819.
[http://dx.doi.org/10.1021/jf903993f] [PMID: 20187608]
[36]
Cieśla, Ł.; Staszek, D.; Kowalska, T.; Waksmundzka-Hajnos, M. The use of TLC-DPPH* test with image processing to study direct antioxidant activity of phenolic acid fractions of selected Lamiaceae family species. J. AOAC Int., 2013, 96(6), 1228-1232.
[http://dx.doi.org/10.5740/jaoacint.SGECiesla] [PMID: 24645498]
[37]
Benedec, D.; Hanganu, D.; Oniga, I.; Tiperciuc, B.; Olah, N.K.; Raita, O.; Bischin, C.; Silaghi-Dumitrescu, R.; Vlase, L. Assessment of rosmarinic acid content in six Lamiaceae species extracts and their antioxidant and antimicrobial potential. Pak. J. Pharm. Sci., 2015, 28(6)(Suppl.), 2297-2303.
[PMID: 26687747]
[38]
Rababah, T.M.; Ereifej, K.I.; Esoh, R.B.; Al-u’datt, M.H.; Alrababah, M.A.; Yang, W. Antioxidant activities, total phenolics and HPLC analyses of the phenolic compounds of extracts from common Mediterranean plants. Nat. Prod. Res., 2011, 25(6), 596-605.
[http://dx.doi.org/10.1080/14786419.2010.488232] [PMID: 21409721]
[39]
Gong, J.; Ju, A.; Zhou, D.; Li, D.; Zhou, W.; Geng, W.; Li, B.; Li, L.; Liu, Y.; He, Y.; Song, M.; Wang, Y.; Ye, Z.; Lin, R. Salvianolic acid Y: A new protector of PC12 cells against hydrogen peroxide-induced injury from Salvia officinalis. Molecules, 2015, 20(1), 683-692.
[http://dx.doi.org/10.3390/molecules20010683] [PMID: 25569522]
[40]
Garcia, C.S.C.; Menti, C.; Lambert, A.P.F.; Barcellos, T.; Moura, S.; Calloni, C.; Branco, C.S.; Salvador, M.; Roesch-Ely, M.; Henriques, J.A.P. Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): Antioxidant, and antitumor in mammalian cells. An. Acad. Bras. Cienc., 2016, 88(1), 281-292.
[http://dx.doi.org/10.1590/0001-3765201520150344] [PMID: 26839997]
[41]
Cocan, I.; Alexa, E.; Danciu, C.; Radulov, I.; Galuscan, A.; Obistioiu, D.; Morvay, A.A.; Sumalan, R.M.; Poiana, M.A.; Pop, G.; Dehelean, C.A. Phytochemical screening and biological activity of Lamiaceae family plant extracts. Exp. Ther. Med., 2018, 15(2), 1863-1870.
[http://dx.doi.org/10.3892/etm.2017.5640] [PMID: 29434776]
[42]
Koşar, M.; Dorman, H.J.; Başer, K.H.; Hiltunen, R. Salvia officinalis L.: composition and antioxidant-related activities of a crude extract and selected sub-fractions. Nat. Prod. Commun., 2010, 5(9), 1453-1456.
[http://dx.doi.org/10.1177/1934578X1000500921] [PMID: 20923007]
[43]
Wang, M.; Shao, Y.; Li, J.; Zhu, N.; Rangarajan, M.; LaVoie, E.J.; Ho, C.T. Antioxidative phenolic glycosides from sage (Salvia officinalis). J. Nat. Prod., 1999, 62(3), 454-456.
[http://dx.doi.org/10.1021/np980436g] [PMID: 10096857]
[44]
Naviglio, D.; Le Grottaglie, L.; Vitulano, M.; Trifuoggi, M.; Gallo, M. Characterization of essential oil components from aromatic plants that grow wild in the “Piana del Sele” (Salerno, Southern Italy) using gas chromatography-mass spectrometry. Nat. Prod. Commun., 2015, 10(7), 1293-1296.
[http://dx.doi.org/10.1177/1934578X1501000739] [PMID: 26411033]
[45]
Williams, J.D.; Yazarians, J.A.; Almeyda, C.C.; Anderson, K.A.; Boyce, G.R. Detection of the previously unobserved stereoisomers of thujone in the essential oil and consumable products of sage (Salvia officinalis L.) using headspace solid-phase microextraction-gas chromatography-mass spectrometry. J. Agric. Food Chem., 2016, 64(21), 4319-4326.
[http://dx.doi.org/10.1021/acs.jafc.6b01065] [PMID: 27181395]
[46]
Craft, J.D.; Satyal, P.; Setzer, W.N. The chemotaxonomy of common sage (Salvia officinalis) based on the volatile constituents. Medicines (Basel), 2017, 4(3), 47.
[http://dx.doi.org/10.3390/medicines4030047] [PMID: 28930262]
[47]
Cvetkovikj, I.; Stefkov, G.; Acevska, J.; Karapandzova, M.; Dimitrovska, A.; Kulevanova, S. Headspace screening: A novel approach for fast quality assessment of the essential oil from culinary sage. Food Chem., 2016, 202, 133-140.
[http://dx.doi.org/10.1016/j.foodchem.2016.01.141] [PMID: 26920277]
[48]
Gudi, G.; Krähmer, A.; Krüger, H.; Schulz, H. Attenuated total Reflectance-Fourier transform infrared spectroscopy on intact dried leaves of sage (Salvia officinalis L.): Accelerated chemotaxonomic discrimination and analysis of essential oil composition. J. Agric. Food Chem., 2015, 63(39), 8743-8750.
[http://dx.doi.org/10.1021/acs.jafc.5b03852] [PMID: 26360136]
[49]
Cvetkovikj, I.; Stefkov, G.; Karapandzova, M.; Kulevanova, S.; Satović, Z. Essential oils and chemical diversity of southeast European populations of Salvia officinalis L. Chem. Biodivers., 2015, 12(7), 1025-1039.
[http://dx.doi.org/10.1002/cbdv.201400273] [PMID: 26172323]
[50]
Martínez-Francés, V.; Hahn, E.; Ríos, S.; Rivera, D.; Reich, E.; Vila, R.; Cañigueral, S. Ethnopharmacological and chemical characterization of Salvia species used in Valencian traditional herbal preparations. Front. Pharmacol., 2017, 8, 467.
[http://dx.doi.org/10.3389/fphar.2017.00467] [PMID: 28790914]
[51]
Cutillas, A.B.; Carrasco, A.; Martinez-Gutierrez, R.; Tomas, V.; Tudela, J. Salvia officinalis L. essential oils from Spain: Determination of composition, antioxidant capacity, antienzymatic, and antimicrobial bioactivities. Chem. Biodivers., 2017, 14(8)e1700102
[http://dx.doi.org/10.1002/cbdv.201700102] [PMID: 28477412]
[52]
Khedher, M.R.B.; Khedher, S.B.; Chaieb, I.; Tounsi, S.; Hammami, M. Chemical composition and biological activities of Salvia officinalis essential oil from Tunisia. EXCLI J., 2017, 16, 160-173.
[http://dx.doi.org/10.17179/excli2016-832] [PMID: 28507464]
[53]
Ben Farhat, M.; Jordán, M.J.; Chaouech-Hamada, R.; Landoulsi, A.; Sotomayor, J.A. Variations in essential oil, phenolic compounds, and antioxidant activity of tunisian cultivated Salvia officinalis L. J. Agric. Food Chem., 2009, 57(21), 10349-10356.
[http://dx.doi.org/10.1021/jf901877x] [PMID: 19886685]
[54]
Pellegrini, M.; Ricci, A.; Serio, A.; Chaves-López, C.; Mazzarrino, G.; D’Amato, S.; Lo Sterzo, C.; Paparella, A. Characterization of essential oils obtained from Abruzzo autochthonous plants: Antioxidant and antimicrobial activities assessment for food application. Foods, 2018, 7(2), 19.
[http://dx.doi.org/10.3390/foods7020019] [PMID: 29393893]

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