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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Phytochemical Investigation and Antimutagenic Potential of Ethanolic Extracts of Emblica officinalis, Terminalia chebula and Terminalia bellirica

Author(s): Venugopal Singamaneni, Sudheer Kumar Dokuparthi, Nilanjana Banerjee*, Ashish Kumar and Tulika Chakrabarti

Volume 10, Issue 4, 2020

Page: [488 - 494] Pages: 7

DOI: 10.2174/2210315509666190618101140

Price: $65

Abstract

Background: Emblica officinalis Gaertn. which belongs to the family Euphorbiaceae, Terminalia chebula Retz. and Terminalia bellerica Roxb. belong to the family Combretaceae. These are well known medicinal plants with phytochemical reservoir of great medicinal values and possess a vast ethnomedical history.

Objective: The aim of the present study is to isolation of major compounds and to evaluate antimutagenic potential of the ethanol extracts of these plants.

Methods: The dried fruits of E. officinalis, T. bellirica and T. chebula were powdered and extracted with 95% ethanol. The ethyl acetate portions were chromatographed over silica gel to isolate major compounds. Antimutagenic activity was determined by Ames test using TA98 and TA100 strains of Salmonella typhimurium.

Results: Two major known compounds, gallic acid and ellagic acid were isolated from the dried fruits of Emblica officinalis, Terminalia chebula and T. bellirica. All the three extracts counteracted the mutagenicity induced by different genotoxic compounds in a dose dependent manner.

Conclusion: This study showed that ethyl acetate portion of three extracts contain two major compounds, gallic acid and ellagic acid which might be responsible for potent antimutagenic activity of these extracts.

Keywords: Antimutagenic activity, Emblica officinalis, Terminalia chebula, Terminalia bellirica, ames test, bacterial strains.

Graphical Abstract

[1]
Ames, B.N.; Durston, W.E.; Yamasaki, E.; Lee, F.D. Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. USA, 1973, 70(8), 2281-2285.
[http://dx.doi.org/10.1073/pnas.70.8.2281] [PMID: 4151811]
[2]
Gupta, S.; Chaudhuri, T.; Seth, P.; Ganguly, D.K.; Giri, A.K. Antimutagenic effects of black tea (World Blend) and its two active polyphenols theaflavins and thearubigins in Salmonella assays. Phytother. Res., 2002, 16(7), 655-661.
[http://dx.doi.org/10.1002/ptr.1038] [PMID: 12410547]
[3]
Mortelmans, K.; Zeiger, E. The Ames Salmonella/microsome mutagenicity assay. Mutat. Res., 2000, 455(1-2), 29-60.
[http://dx.doi.org/10.1016/S0027-5107(00)00064-6] [PMID: 11113466]
[4]
Bhagavathy, S.; Sumathi, P.; Madhushree, M. Antimutagenic assay of carotenoids from green algae Chlorococcum humicola using Salmonella typhimurium TA98, TA100 and TA102. Asian Pac. J. Trop. Dis., 2011, 1, 308-316.
[http://dx.doi.org/10.1016/S2222-1808(11)60073-X]
[5]
Edenharder, R.; von Petersdorff, I.; Rauscher, R. Antimutagenic effects of flavonoids, chalcones and structurally related compounds on the activity of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and other heterocyclic amine mutagens from cooked food. Mutat. Res., 1993, 287(2), 261-274.
[http://dx.doi.org/10.1016/0027-5107(93)90019-C] [PMID: 7685486]
[6]
Kada, T.; Kaneko, K.; Matsuzaki, S.; Matsuzaki, T.; Hara, Y. Detection and chemical identification of natural bio-antimutagens. A case of the green tea factor. Mutat. Res., 1985, 150(1-2), 127-132.
[http://dx.doi.org/10.1016/0027-5107(85)90109-5] [PMID: 3923334]
[7]
Sakai, Y.; Nagase, H.; Ose, Y.; Kito, H.; Sato, T.; Kawai, M.; Mizuno, M. Inhibitory action of peony root extract on the mutagenicity of benzo[a]pyrene. Mutat. Res., 1990, 244(2), 129-134.
[http://dx.doi.org/10.1016/0165-7992(90)90061-N] [PMID: 2192283]
[8]
Imanishi, H.; Sasaki, Y.F.; Ohta, T.; Watanabe, M.; Kato, T.; Shirasu, Y. Tea tannin components modify the induction of sister-chromatid exchanges and chromosome aberrations in mutagen-treated cultured mammalian cells and mice. Mutat. Res., 1991, 259(1), 79-87.
[http://dx.doi.org/10.1016/0165-1218(91)90111-X] [PMID: 1899132]
[9]
Tanaka, K.; Hayatsu, T.; Negishi, T.; Hayatsu, H. Inhibition of N-nitrosation of secondary amines in vitro by tea extracts and catechins. Mutat. Res., 1998, 412(1), 91-98.
[http://dx.doi.org/10.1016/S1383-5718(97)00178-2] [PMID: 9508368]
[10]
Jain, R.; Pandey, R.; Mahant, R.N.; Rathore, D.S. A review on medicinal importance of Emblica officinalis. Int. J. Pharm. Sci. Res., 2015, 6, 72-84.
[11]
Khan, K.H. Roles of Emblica officinalis in Medicine - A review. Bot. Res. Int., 2009, 2, 218-228.
[12]
Aruna, S.; Nandakishore, L.V. Haritaki a boon to herbalism- A review. Sch. Acad. J. Biosci., 2014, 2, 132-136.
[13]
Gupta, P.C. Biological and pharmacological properties of Terminalia chebula retz. (Haritaki)- An overview. Int. J. Pharm. Pharm. Sci., 2012, 4, 62-68.
[14]
Rathinamoorthy, R.; Thilagavathi, G. Terminalia chebula- Review on pharmacological and biochemical studies. Int. J. Pharm. Tech. Res., 2014, 6, 97-116.
[15]
Saraswathi, M.N.; Karthikeyan, M.; Kannan, M.; Rajasekhar, S. Terminalia belerica. Roxb- A phytopharmacological review. Int. J. Res. Pharm. Biomed. Sci., 2012, 3, 96-99.
[16]
Deb, A.; Barua, S.; Das, B. Pharmacological activities of Baheda (Terminalia bellerica): A review. J. Pharmacog. Phytochem., 2015, 5, 194-197.
[17]
Mallik, J.; Das, P.; Karon, B.; Das, S. A review on phytochemistry and pharmacological activity of Terminalia belerica. Int. J. Drug. For. Res., 2012, 3, 1-7.
[18]
Maron, D.M.; Ames, B.N. Revised methods for the Salmonella mutagenicity test. Mutat. Res., 1983, 113(3-4), 173-215.
[http://dx.doi.org/10.1016/0165-1161(83)90010-9] [PMID: 6341825]
[19]
Dokuparthi, S.K.; Banerjee, N.; Kumar, A.; Singamaneni, V.; Giri, A.K.; Mukhopadhyay, S. Phytochemical investigation and evaluation of antimutagenic activity of the extract of Cuscuta reflexa Roxb by ames test. Int. J. Pharm. Sci. Res., 2014, 5, 3430-3434.
[20]
Bhattacharya, U.; Mukhopadhyay, S.; Giri, A.K. Comparative antimutagenic and anticancer activity of three fractions of black tea polyphenols thearubigins. Nutr. Cancer, 2011, 63(7), 1122-1132.
[http://dx.doi.org/10.1080/01635581.2011.605985] [PMID: 21919645]
[21]
Chanwitheesuk, A.; Teerawutgulrag, A.; Kilburn, J.D.; Rakariyatham, N. Antimicrobial gallic acid from Caesalpinia mimosoides Lamk. Food Chem., 2007, 100, 1044-1048.
[http://dx.doi.org/10.1016/j.foodchem.2005.11.008]
[22]
Srivastava, A.; Jagan Mohan Rao, L.; Shivanandappa, T. Isolation of ellagic acid from the aqueous extract of the roots of Decalepis hamiltonii: Antioxidant activity and cytoprotective effect. Food Chem., 2007, 103, 224-233.
[http://dx.doi.org/10.1016/j.foodchem.2006.08.010]
[23]
de Mejía, E.G.; Castaño-Tostado, E.; Loarca-Piña, G. Antimutagenic effects of natural phenolic compounds in beans. Mutat. Res., 1999, 441(1), 1-9.
[http://dx.doi.org/10.1016/S1383-5718(99)00040-6] [PMID: 10224317]
[24]
Hour, T.C.; Liang, Y.C.; Chu, I.S.; Lin, J.K. Inhibition of eleven mutagens by various tea extracts, (-)epigallocatechin-3-gallate, gallic acid and caffeine. Food Chem. Toxicol., 1999, 37(6), 569-579.
[http://dx.doi.org/10.1016/S0278-6915(99)00031-9] [PMID: 10478825]
[25]
Kaur, S.; Grover, I.S.; Kumar, S. Antimutagenic potential of ellagic acid isolated from Terminalia arjuna. Indian J. Exp. Biol., 1997, 35(5), 478-482.
[PMID: 9378517]

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