Login Register Cart 0
Generic placeholder image

Anti-Cancer Agents in Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

A Cytotoxic Natural Product from Patrinia villosa Juss

Author(s): Yangcheng Liu, Wei Liu, Changlan Chen, Zheng Xiang* and Hongwei Liu*

Volume 19, Issue 11, 2019

Page: [1399 - 1404] Pages: 6

DOI: 10.2174/1871520619666190416101014

Price: $65

Abstract

Background and Purpose: Patrinia villosa Juss is an important Chinese herbal medicine widely used for thousands of years, but few reports on the ingredients of the herb have been presented. In this study, we aim to isolate the bioactive compound from the plant.

Material and Methods: The air-dried leaves of P. villosa (15kg) were extracted three times with 70% EtOH under reflux. The condensed extract was suspended in H2O and partitioned with light petroleum, dichloromethane and n-BuOH. The dichloromethane portion was then subjected to normal-phase silica gel column chromatography, ODS silica gel column chromatography and semi-preparative HPLC to yield compound 1. Cytotoxicities of 1 were assayed on HepG2, A549 and A2780 cell lines. The mechanism of apoptosis and cell cycle on A549 was confirmed subsequently.

Results: A new impecylone (Impecylone A) was isolated from the leaves of Patrinia villosa Juss, and its structures were established using 1D, 2D-NMR spectra and HR-ESI-MS. Impecylone A could selectivity inhibit HepG2 and A549 cell lines. The compound could induce apoptosis of A549 and arrest the cell cycle at G2/M phase in a dose-dependent manner.

Conclusion: Impecylone A is a novel compound from Patrinia villosa Juss and could be a potential antitumor agent especially in the cell lines of A549.

Keywords: Patrinia villosa Juss, impecylone, nuclear magnetic resonance, cytotoxicity, apoptosis, cell cycle.

« Previous Next »
Graphical Abstract

[1]
Cui, W.Y.; Liu, S.X.; Song, X.K.; Liu, D.L.; Zhang, T.J. Research progress on chemical constituents and pharmacological effects of Patrinia scabiosaefolia and Patrinia villosa. Drug. Eval. Res, 2016, 39(3), 482-488.
[2]
Wang, Q. Research progress of Patrinia villosa and Patrinia scabiosaefolia. Med. Recapitulate, 2009, 15(13), 2021-2022.
[3]
Chiu, L.C.; Ho, T.S.; Wong, Y.L.; Ooi, E.C. Ethyl acetate extract of Patrinia scabiosaefolia downregulates anti-apoptotic Bcl-2/Bcl-X(L) expression, and induces apoptosis in human breast carcinoma MCF-7 cells independent of caspase-9 activation. J. Ethnopharmacol., 2006, 105(1-2), 1-268.
[4]
Peng, J.Y.; Fan, G.R.; Wu, Y.T. Isolation and separation of flavones from Patrinia villosa by high-speed counter-current chromatography. Ch. Pharm. J., 2006, 41(13), 977-979.
[5]
Yan, X.J.; Liu, W.; Zhao, Y.; Chen, N.; Xu, Y.; Wu, J.; Wang, T.; Li, Y.; Xiang, Z. A new biphenyl neolignan from leaves of Patrinia villosa (Thunb.) Juss. Pharmacogn. Mag., 2016, 12(45), 1-3.
[6]
Yang, Y.F.; Ma, H.M.; Chen, G.; Wang, H.F.; Xiang, Z.; Feng, Q.M.; Hua, H.M.; Pei, Y.H. A new sesquiterpene lactone glycoside and a new quinic acid methyl ester from Patrinia villosa. J. Asian Nat. Prod. Res., 2016, 18(10), 945-951.
[7]
Xiang, Z.; Zhao, S.S.; Zhao, Y.; Li, N.; Wu, J.; Chen, C.L.; Liu, H.W. Chemical constituents from Patrinia villosa (Thunb.) Juss. Lat. Am. J. Pharm., 2017, 36(12), 2425-2430.
[8]
Xiang, Z.; Chen, N.; Xu, Y.; Wu, J.; Liu, Y.J.; Tan, C.; Ji, Y.B.; Li, W.L. New flavonoid from Patrinia villosa. Pharm. Biol., 2016, 54(7), 1219-1222.
[9]
Yan, X.J.; Zheng, W.; Wen, J.; Wu, J.; Xu, Y.; Cao, L.; Wang, X.H.; Xiang, Z. Chemical constituents of Patrinia villosa. Chin. Tradit. Herbal Drugs, 2017, 48(2), 247-251.
[10]
Xiang, X.; Yan, J.X.; Wen, J.; Liu, W. Chemical constituents of whole plants of Patrinia villosa (Thunb.) Juss. Chin. Pharm. J, 2017, 52(3), 185-187.
[11]
Bao, Y.R.; Yan, X.J.; Yang, X.X.; Wang, S.; Zhao, L.; Bai, M.; Xiang, Z. Study on chemical constituents of Patrinia villosa. Chin. Med. Mat, 2017, 40(2), 347-349.
[12]
Yan, X.J.; Zheng, W.; Wen, J.; Wu, J.; Bai, M.; Xiang, Z. Lignan constituents of Patrinia villosa (Thunb.) Juss. Chin. Pharm. J, 2017, 52(13), 1126-1131.
[13]
Liu, X.; Zhang, B.F.; Yang, L.; Chou, G.X.; Wang, Z.T. Four new compounds from Imperata cylindrica. J. Nat. Med., 2014, 68(2), 295-301.
[14]
Jiang, D.; Chiaro, C.; Maddali, P.; Prabhu, K.S.; Peterson, D.G. Identification of hydroxycinnamic acid-maillard reaction products in low-moisture baking model systems. J. Agric. Food Chem., 2009, 57(21), 9932-9943.

Rights & Permissions Print Cite
Article Metrics
42
3
© 2024 Bentham Science Publishers | Privacy Policy