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Letter Article

FoxF1是Cilium中Sonic Hedgehog信号组件的Ciliogenesis和分布所必需的

卷 19, 期 5, 2019

页: [326 - 334] 页: 9

弟呕挨: 10.2174/1566524019666190405115420

价格: $65

摘要

背景：在脊椎动物中，纤毛对于Hedgehog信号转导至关重要。据报道，在许多情况下，叉头盒转录因子FoxF1与Sonic Hedgehog（Shh）信号传导有关。然而，FoxF1在纤毛中的作用仍然未知。在这里，我们展示了FoxF1在纤毛发生的调节和纤毛中Shh信号组分分布中的重要作用。 方法：NIH / 3T3细胞血清饥饿24h诱导纤毛。同时，shRNA用于敲低细胞中的FoxF1表达，并且CRISPR / Cas9用于产生FoxF1斑马鱼突变体。通过qRT-PCR和蛋白质印迹分别检测指示基因的mRNA和蛋白质表达。进行免疫荧光染色以检测纤毛和Shh组分分布。 结果：FoxF1敲低降低了NIH / 3T3细胞的纤毛长度。同时，FoxF1功能的破坏抑制了纤毛相关基因的表达，并导致纤毛中Shh成分的异常分布。此外，纯合的FoxF1突变体在早期斑马鱼胚胎中表现出缺陷性纤毛的缺陷。 结论：总之，我们的数据表明FoxF1是体外和体内纤毛发生所必需的，并且是纤毛中Shh信号组分的正确定位所必需的。

关键词: FoxF1，纤毛，ciliogenesis，Sonic Hedgehog，斑马鱼突变体，hypephric cilium。

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[http://dx.doi.org/10.1016/S0091- 679X(10)97022-2.]

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