摘要
背景：肝激酶B1（LKB1）/ 5''-腺苷单磷酸激活蛋白激酶（AMPK）信号传导是一种代谢检查点，在阿尔茨海默病（AD）的发病机制中起着神经保护作用。淀粉样蛋白-β（Aβ）充当AD的经典生物标志物。本研究的目的是探讨小檗碱（BBR）是否激活LKB1 / AMPK信号并改善Aβ病理。 方法：采用酶联免疫吸附试验和免疫组化法检测Aβ水平。通过蛋白质印迹测量以下生物标志物：磷酸化（p-）LKB1（Ser334和Thr189），p-AMPK（AMPKα和AMPKβ1），突触素，突触后密度蛋白95和p-cAMP-反应元件结合蛋白（p- CREB）。使用蛋白质印迹和免疫组织化学测定胶质原纤维酸性蛋白（GFAP）。 结果：BBR抑制APP / PS1小鼠脑内Aβ的表达。在BBR处理后，APP / PS1转基因小鼠的脑中p-LKB1（Ser334和Thr189）和p-AMPK（AMPKα和AMPKβ1）均有强烈的上调（P <0.01）。与模型小鼠相比，BBR促进AD脑中突触素，突触后密度蛋白95和p-CREB（Ser133）的表达。 结论：BBR通过激活APP / PS1转基因小鼠脑内LKB1 / AMPK信号通路，减轻Aβ发病机制，挽救突触损伤。
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