摘要
背景：考虑到外泌体作为细胞间转运蛋白，不可避免地与质膜和大量可用的血细胞表面相互作用，我们想知道一小部分循环外泌体是否与血细胞表面有关。 目的：本研究的目的是开发一种有效的方案，用于分离与血细胞表面相关的外泌体，并进一步研究该部分在健康状态和乳腺癌发展过程中的特征，以及其可能的意义。用于诊断应用。 方法：从健康女性（HF）和乳腺癌患者（BCP）收集血液样本。从血浆中提取的外泌体和从血细胞表面洗脱的外体通过超滤分离，随后进行超速离心。 结果：透射电子显微镜（TEM）以及免疫金标记证明，从血浆和血细胞洗脱液中分离的膜包裹的细胞外囊泡（EV）中存在外泌体。 TEM，纳米粒子追踪分析和NanoOrange蛋白质定量数据显示，细胞相关外泌体构成不少于总血液外泌体数量的2/3。大约50-70纳米的外泌体在乳腺癌患者的血液中占优势，而较小的外泌体（30-50纳米）主要在健康女性的血液中观察到。对在血液中循环的外来体中的特定蛋白质和RNA的分析证明了HF和BCP的外来体中聚合物的堆积密度的显着差异。初步数据表明，检测与红细胞分数相关的外泌体中的癌症特异性miRNA（miR-103，miR-191，miR-195），与血浆中循环的无细胞外泌体相比，可以更准确地区分HF和BCP。 。 结论：我们的数据为使用血细胞相关外泌体进行诊断应用提供了基础。
关键词: 外泌体，细胞外囊泡，血细胞，细胞相关外泌体，miRNA，乳腺癌。
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