Abstract
The use of genomics tools to discover new genes, to decipher pathways or to assign a function to a gene is just beginning to have an impact. Genomics approaches have been applied to both antibacterial and antifungal target discovery in order to identify a new generation of antibiotics. This review discusses genomics approaches for antifungal drug discovery, focusing on the areas of gene discovery, target validation, and compound screening. A variety of methods to identify fungal genes of interest are discussed, as well as methods for obtaining full-length sequences of these genes. One approach is well-suited to organisms having few introns (Candida albicans), and another for organisms with many introns (Aspergillus fumigatus). To validate broad spectrum fungal targets, the yeast Saccharomyces cerevisiae was used as a model system to rapidly identify genes essential for growth and viability of the organism. Validated targets were then exploited for high-throughput compound screening.
Keywords: antifungal, antibacterial, target validation, fungal genes, bacterial genes
Current Pharmaceutical Design
Title: Genomics Strategies for Antifungal Drug Discovery - From Gene Discovery to Compound Screening
Volume: 8 Issue: 13
Author(s): Debra Aker Willins, Marco Kessler, Scott S. Walker, Gregory R. Reyes and Guillaume Cottarel
Affiliation:
Keywords: antifungal, antibacterial, target validation, fungal genes, bacterial genes
Abstract: The use of genomics tools to discover new genes, to decipher pathways or to assign a function to a gene is just beginning to have an impact. Genomics approaches have been applied to both antibacterial and antifungal target discovery in order to identify a new generation of antibiotics. This review discusses genomics approaches for antifungal drug discovery, focusing on the areas of gene discovery, target validation, and compound screening. A variety of methods to identify fungal genes of interest are discussed, as well as methods for obtaining full-length sequences of these genes. One approach is well-suited to organisms having few introns (Candida albicans), and another for organisms with many introns (Aspergillus fumigatus). To validate broad spectrum fungal targets, the yeast Saccharomyces cerevisiae was used as a model system to rapidly identify genes essential for growth and viability of the organism. Validated targets were then exploited for high-throughput compound screening.
Export Options
About this article
Cite this article as:
Willins Aker Debra, Kessler Marco, Walker S. Scott, Reyes R. Gregory and Cottarel Guillaume, Genomics Strategies for Antifungal Drug Discovery - From Gene Discovery to Compound Screening, Current Pharmaceutical Design 2002; 8 (13) . https://dx.doi.org/10.2174/1381612023394890
DOI https://dx.doi.org/10.2174/1381612023394890 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Chemical Characterization and Antioxidant Potential of <i>Athroisma proteiformis</i> Essential Oil
The Natural Products Journal A SAR Study: Evaluation of Bromo Derivatives of 8-Substituted Quinolines as Novel Anticancer Agents
Letters in Drug Design & Discovery Phytochemical Screening and Antibacterial Activity of Aloe Vera Collected from Different Climatic Regions of India
The Natural Products Journal Antioxidants in Health, Disease and Aging
CNS & Neurological Disorders - Drug Targets Rhenium-188 and Copper-67 Radiopharmaceuticals for the Treatment of Bladder Cancer
Mini-Reviews in Medicinal Chemistry New Insights in the Gene Electrotransfer Process: Evidence for the Involvement of the Plasmid DNA Topology
Current Gene Therapy The Ultrasonographic Diagnosis of Polycystic Ovary Syndrome
Current Medical Imaging Phytochemicals for Drug Discovery in Alzheimer’s Disease: <i>In Silico</i> Advances
Current Pharmaceutical Design Understanding Recognition and Self-assembly in Biology using the Chemist´s Toolbox. Insight into Medicinal Chemistry
Current Topics in Medicinal Chemistry Interaction of Anthocyanins with Drug-metabolizing and Antioxidant Enzymes
Current Medicinal Chemistry Mass Spectroscopic Methods for the Characterization of Flavonoid Compounds
Current Bioactive Compounds Aptamers as Therapeutics in Cardiovascular Diseases
Current Medicinal Chemistry Microdialysis: A Technique for Pharmacokinetic-Pharmacodynamic Studies of Oncological Drugs
Current Pharmaceutical Biotechnology Trypanosomal dUTPases as Potential Targets for Drug Design
Current Protein & Peptide Science Chromatographic Fingerprinting and Metabolomics for Quality Control of TCM
Combinatorial Chemistry & High Throughput Screening Chemical Screening and Biological Activities of Extracts from <i>Cistus munbyi</i> Pomel
Current Traditional Medicine Microwave-Assisted Synthesis, Antimicrobial and Cytotoxic Activities of Some 4-Thioxo-Thiazolidine-2-One Derivatives
Letters in Drug Design & Discovery Reproductive Toxicology of Environmental Toxicants: Emerging Issues and Concerns
Current Pharmaceutical Design Downstream Processing of Antibodies: Potential of Affinity Chromatography
Current Biochemical Engineering (Discontinued) Imaging Drug Resistance with Radiolabeled Molecules
Current Pharmaceutical Design