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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Sequential Injection Chromatography with Monolithic Column for Phenothiazines Assay in Human Urine and Pharmaceutical Formulations

Author(s): Abubakr M. Idris*

Volume 16, Issue 7, 2020

Page: [967 - 975] Pages: 9

DOI: 10.2174/1573412915666190219143750

Price: $65

Abstract

Background: Sequential injection chromatography (SIC) with monolithic column has been proposed with potential benefits for separation and quantification.

Objective: To utilize SIC to develop a new assay method for the separation and quantification of some phenothiazines (promethazine, chlorpromazine and perphenazine) in human urine and synthetic pharmaceutical formulations.

Methods: The 32 full-factorial design was adopted to study the effect of mobile phase composition on separation efficiency, retention time, peak height and baseline. The separation was conducted on a C18 monolithic column (100 × 4.6 mm) using a mobile phase composition of phosphate: acetonitrile:methanol (60:28:12) at pH 4.0. The detection was carried out using a miniaturized fiber optic spectrometer at 250 nm.

Results: Satisfactory analytical features, including number of theoretical plates (1809-6232), peak symmetry (1.0-1.3), recovery (95.5-99.1% in pharmaceutical formulations and 91.6-94.7% in urine), intra-day precision (0.36-1.60% for pharmaceutical formulation and 2.96-3.67 for urine), inter-day precision (1.47-2.28% for pharmaceutical formulation), limits of detection (0.23-0.88 μg/ml) and limits of quantification (0.77-2.90 μg/ml), were obtained.

Conclusion: The remarkable advantages of the proposed SIC method are the inexpensiveness in terms of instrumentation and reagent consumption.

Keywords: Sequential injection chromatography, bioanalysis, pharmaceutical analysis, phenothiazines, monolithic column, human urine.

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[1]
Dahl, S.G. in:clinical pharmacology and psychiatry, neuroleptic antidepres-sants research (2nd international meeting). macmillan, lon-don; usdin, e; dahl, s.g; gram, l.f, eds., 1981, p. 125-137.
[2]
Baldessarini, R.J. The pharmacological basis of therapeutics, 7thed; gilman, a.g.; goodman, l.s.; rall, t.w.; murad, f., eds.; macmillan: new york, , 1985. pp; 391-, 423, 426;
[3]
Darwish, H.W.; Naguib, I.A.; Bakheit, A.H. Stability Indicating spectrofluorimetric analysis of metopimazine by signal enhanced - partial least squares chemometric models: a comparative study. Curr. Pharm. Anal., 2016, 12(3), 234-243.
[http://dx.doi.org/10.2174/1573412912666151207185931]
[4]
Seubert, A.; Rasheed, A.S. Separation of metal–trifluoperazine hydrochloride complexes using Zwitterionic Ion Chromatography (ZIC) coupled online with ICP-AES. Curr. Pharm. Anal., 2017, 13(4), 328-333.
[http://dx.doi.org/10.2174/1573412912666160720114147]
[5]
Aoki, R.; Arinobu, T.; Kumazawa, T.; Hattori, H.; Noguchi, H. An automated on-line method for simultaneous analysis of phenothiazines in human serum by high-performance liquid chromatography/sonic spray ionization mass spectrometry using backflush column switching. Forensic Toxicol., 2007, 25(1), 8-15.
[http://dx.doi.org/10.1007/s11419-007-0020-z]
[6]
Lara, F.J.; García-Campaña, A.M.; Alés-Barrero, F.; Bosque-Sendra, J.M. Development and validation of a capillary electrophoresis method for the determination of phenothiazines in human urine in the low nanogram per milliliter concentration range using field-amplified sample injection. Electrophoresis, 2005, 26(12), 2418-2429.
[http://dx.doi.org/10.1002/elps.200500098] [PMID: 15924366]
[7]
Moffat, A.C.; Osselton, M.D.; Widdop, B.; Galichet, L.Y., Eds.; Clarke’s analysis of drugs and poisons; Pharmaceutical Press: London, 2004.
[8]
Randall, C. disposition of toxic drugs and chemical in man, baselt bio-medical publications, 6th ed; foster city, california, usa , 2002.
[9]
Ruzicka, J.; Marshall, G.D. Sequential injection: a new concept for chemical sensors, process analysis and laboratory assays. Anal. Chim. Acta, 1990, 237, 329-343.
[http://dx.doi.org/10.1016/S0003-2670(00)83937-9]
[10]
Idris, A.M. The second five years of sequential injection chromatography: significant developments in the technology and methodologies. Crit. Rev. Anal. Chem., 2014, 44(3), 220-232.
[http://dx.doi.org/10.1080/10408347.2013.848778] [PMID: 25391562]
[11]
Saurina, J. Flow-injection analysis for multi-component determinations of drugs based on chemometric approaches. Trends Analyt. Chem., 2010, 29(9), 1027-1037.
[http://dx.doi.org/10.1016/j.trac.2010.05.012]
[12]
Worsfold, P.J.; Clough, R.; Lohan, M.C.; Monbet, P.; Ellis, P.S.; Quétel, C.R.; Floor, G.H.; McKelvie, I.D. Flow injection analysis as a tool for enhancing oceanographic nutrient measurements--a review. Anal. Chim. Acta, 2013, 803, 15-40.
[http://dx.doi.org/10.1016/j.aca.2013.06.015] [PMID: 24216194]
[13]
Ma, J. Yuan, Lin, K.; Feng, S.; Zhou, T.; Li, Q. Applications of flow techniques in seawater analysis: a review. Trends Environ. Anal. Chem., 2016, 10, 1-10.
[http://dx.doi.org/10.1016/j.teac.2016.02.003]
[14]
López-Paz, J.L.; Catalá-Icardo, M. Analysis of pesticides by flow injection coupled with chemiluminescent detection: A Review. Anal. Lett., 2011, 44(1-3), 146-175.
[http://dx.doi.org/10.1080/00032719.2010.500788]
[15]
Idris, A.M. Flow injection, overlooked techniques in forensic analysis. Crit. Rev. Anal. Chem., 2010, 40(4), 218-225.
[http://dx.doi.org/10.1080/10408347.2010.515448]
[16]
Satinsky, D.; Solich, P.; Chocholous, P.; Karlicek, R. Monolithic columns—a new concept of separation in the sequential injection technique. Anal. Chim. Acta, 2003, 499, 205-214.
[http://dx.doi.org/10.1016/S0003-2670(03)00625-1]
[17]
Idris, A.M.; Elgorashe, R.E.E. Sequential injection chromatography with a miniaturized multi-channel fiber optic detector for separation and quantification of propranolol and hydrochlorothiazide. Chem. Cent. J., 2011, 5, 28.
[http://dx.doi.org/10.1186/1752-153X-5-28] [PMID: 21658210]
[18]
Idris, A.M.; Elgorashe, R.E.E.; Assubaie, F.N.; Alnajjar, A.O. Inexpensive green method for diclofenac assay utilizing sequential injection chromatography. Chromatographia, 2011, 73(5-6), 431-437.
[http://dx.doi.org/10.1007/s10337-011-1936-3]
[19]
Idris, A.M.; Elgorashe, R.E.E. Sequential injection chromatography against hplc and ce: application to separation and quantification of amoxicillin and clavulanic acid. Microchem. J., 2011, 99(2), 174-179.
[http://dx.doi.org/10.1016/j.microc.2011.04.011]
[20]
Idris, A.M.; Naheid, S.A.; Eltayeb, M.A.H.; Elgorashe, R.E.E.; Al-Akra, H.N. Reversed-phase sequential injection liquid chromatographic method for Sildenafil Assay. J. Liq. Chromatogr. Relat. Technol., 2011, 34(19), 2256-2270.
[http://dx.doi.org/10.1080/10826076.2011.587222]
[21]
Idris, A.M.; Naheid, S.A.; Elgorashe, R.E.E.; Alnajjar, A.O.; Eltayeb, M.A.H. Multi-response optimization of sequential injection chromatographic method for simultaneous determination of lisinopril and hydrochlorothiazide. Anal. Methods, 2012, 4(7), 2081-2087.
[http://dx.doi.org/10.1039/c2ay05876f]
[22]
Elgorashe, R.E.E.; Idris, A.M.; Abdelrahman, M.A.; Saeed, A.E.M. Micro-scale method for the separation and quantification of atenolol and hydrochlorothiazide by sequential injection chromatography. J. Liq. Chromatogr. Relat. Technol., 2013, 36(19), 2814-2827.
[http://dx.doi.org/10.1080/10826076.2012.725699]
[23]
Elgorashe, R.E.E.; Idris, A.M.; Abdelrahman, M.A.; Saeed, A.E.M. Facile assay method for norfloxacin and ciprofloxacin by sequential injection chromatography. Acta Chromatogr., 2014, 26(2), 321-334.
[http://dx.doi.org/10.1556/AChrom.26.2014.2.9]
[24]
Idris, A.M.; Alnajjar, A.O. Optimizing SIC assay method for Acetyl Salicylic Acid and Rosuvastatin; and adapting to HPLC with performance comparison. Acta Chromatogr., 2015, 27(1), 111-125.
[http://dx.doi.org/10.1556/AChrom.27.2015.1.9]
[25]
Karakosta, T.D.; Tzanavaras, P.D.; Themelis, D.G. Automated determination of total captopril in urine by liquid chromatography with post-column derivatization coupled to on-line solid phase extraction in a sequential injection manifold. Talanta, 2012, 88, 561-566.
[http://dx.doi.org/10.1016/j.talanta.2011.11.034] [PMID: 22265541]
[26]
Idris, A.M. Sequential injection chromatography for biofluidic analysis: application to promethazine assay. J. Liq. Chromatogr. Relat. Technol., 2012, 35(20), 2884-2899.
[http://dx.doi.org/10.1080/10826076.2011.639119]
[27]
Idris, A.M.; Elgorashe, R.E.E. Sequential injection chromatography for separation and quantification of chlorpromazine in human urine and pharmaceutical formulations. J. AOAC Int., 2013, 96(2), 282-289.
[http://dx.doi.org/10.5740/jaoacint.11-057] [PMID: 23767351]
[28]
Idris, A.M.; Alnajjar, A.O. Native fluorescent detection with sequential injection chromatography for doping control analysis. Chem. Cent. J., 2013, 7(1), 144.
[http://dx.doi.org/10.1186/1752-153X-7-144] [PMID: 23985079]
[29]
Šrámková, I.; Chocholouš, P.; Sklenářová, H.; Šatínský, D. On-line coupling of micro-extraction by packed sorbent with sequential injection chromatography system for direct extraction and determination of betaxolol in human urine. Talanta, 2015, 143, 132-137.
[http://dx.doi.org/10.1016/j.talanta.2015.05.048] [PMID: 26078140]
[30]
Naheed, S.A.; Idris, A.M.; Elgorashe, R.E.E.; Altayeb, M.A.H.; Alnajjar, A.O.; Assubaie, F.N. High-throughput reagent-saving sequential injection assay method for chlorpromazine. J. Anal. Chem., 2013, 68(3), 252-259.
[31]
Idris, A.M. Factorial design and response surface optimization of a spectrophotometric sequential injection analysis of olanzapine. J. Anal. Chem., 2010, 65(1), 36-42.
[http://dx.doi.org/10.1134/S1061934810010089]
[32]
Idris, A.M.; Elgorashe, R.E.E.; Alnajjar, A.O. Developing new method for quantifying pindolol by sequential injection analysis. J. Anal. Chem., 2012, 67(5), 497-503.
[http://dx.doi.org/10.1134/S1061934812030021]
[33]
Idris, A.M.; Assubaie, F.N.; Sultan, S.M. experimental design optimization of a sequential injection method for promazine assay in bulk and pharmaceutical formulations j. auto. methods manage. chem, 2007. Article id 32470.,
[34]
Idris, A.M.; Assubaie, F.N.; Sultan, S.M. Chemometric optimization of a SIA promethazine hydrochloride assay method. Microchem. J., 2006, 83(1), 7-13.
[http://dx.doi.org/10.1016/j.microc.2005.12.004]
[35]
Idris, A.M. On-line coupling of solid-phase extraction, derivatization reaction and spectrophotometry by sequential injection analysis: application to trifluoperazine assay in human urine. J. Pharmacol. Toxicol. Methods, 2007, 56(3), 330-335.
[http://dx.doi.org/10.1016/j.vascn.2007.08.005] [PMID: 17897843]
[36]
Idris, A.M.; Ibrahim, A.E.E.; Abulkibash, A.M.; Saleh, T.A.; Ibrahim, K.E.E. Rapid inexpensive assay method for verapamil by spectrophotometric sequential injection analysis. Drug Test. Anal., 2011, 3(6), 380-386.
[http://dx.doi.org/10.1002/dta.277] [PMID: 21452175]
[37]
Cruz-Vera, M.; Lucena, R.; Cárdenas, S.; Valcárcel, M. Determination of phenothiazine derivatives in human urine by using ionic liquid-based dynamic liquid-phase microextraction coupled with liquid chromatography. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2009, 877(1-2), 37-42.
[http://dx.doi.org/10.1016/j.jchromb.2008.11.017] [PMID: 19042163]
[38]
Tanaka, E.; Nakamura, T.; Terada, M.; Shinozuka, T.; Hashimoto, C.; Kurihara, K.; Honda, K. Simple and simultaneous determination for 12 phenothiazines in human serum by reversed-phase highperformance liquid chromatography. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, 854(1-2), 116-120.
[http://dx.doi.org/10.1016/j.jchromb.2007.04.004] [PMID: 17459789]
[39]
Hayen, H.; Karst, U. Analysis of phenothiazine and its derivatives using LC/electrochemistry/MS and LC/electrochemistry/fluorescence. Anal. Chem., 2003, 75(18), 4833-4840.
[http://dx.doi.org/10.1021/ac0346050] [PMID: 14674461]
[40]
ich harmonised tripartite guideline validation of analytical procedures: text and methodology q2 (r1). parent guideline dated october 27, 1994 (complementary guideline on meth-odology dated november 6, 1996 incorporated in november,, 2005.
[41]
International union of pure and applied chemistry. Harmonized guidelines for single laboratory validation of methods of analysis (IUPAC Technical Report). Pure Appl. Chem., 2002, 74(2), 835-855.
[42]
Piperaki, S.; Perakis, A.; Parissi-Poulou, M. Liquid chromatographic retention behaviour and separation of promethazine and isopromethazine on a β-cyclodextrin bonded-phase column. J. Chromatogr. A, 1994, 660(1-2), 339-350.
[http://dx.doi.org/10.1016/0021-9673(94)85129-8]

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