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Review Article

如何荧光标记钾离子通道:一个hERG病例

卷 27, 期 18, 2020

页: [3046 - 3054] 页: 9

弟呕挨: 10.2174/0929867326666181129094455

价格: $65

摘要

hERG(Human ether-a-go-go-related gene)钾通道在心脏动作电位复极化中发挥重要作用，是遗传和药物诱导的长QT综合征的原因。最近，捕获了hERG通道开放构象的低温电镜结构被确定，从而推动了对3.8 A分辨率的hERG通道的研究。本报告主要总结了几种以hERG通道为靶点的荧光探针的设计原理和应用，这些荧光探针能够对钾离子通道亲和性进行动态和实时监测，从而进一步加深对通道的理解。

关键词: hERG通道，小分子荧光探针，开启开关，荧光团，识别基序，低温电子显微镜结构。

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