摘要
RNA干扰在疾病预防中的广泛应用依赖于以经济上可行，可扩展和可持续的方式生产dsRNA，以及鉴定安全有效的RNA递送方法。目前的研究引起人们对酿酒酵母（Saccharomyces cerevisiae）用于这些应用的兴趣。该综述研究了酵母干扰RNA表达和递送系统的商业开发潜力。酿酒酵母是缺乏功能性RNA干扰系统的遗传模型生物，其可以使其成为表达和积累高水平重组干扰RNA的理想系统。此外，最近在各种真核物种中的研究表明，这种微生物可能是干扰RNA递送的优良且安全的系统。进一步研究和开发的关键领域包括优化酿酒酵母中的干扰RNA表达，表达干扰RNA分子的重组酵母培养物的工业大小比例，用于保持干扰RNA完整性的酵母的大规模干燥方法的开发，以及鉴定在各种环境条件下促进酵母稳定性的包封剂。酿酒酵母的遗传易处理性以及在食品和制药工业中使用这种微生物的悠久历史将促进这种有前景的新技术的进一步发展，该技术具有许多具有医学重要性的潜在应用。
关键词: RNAi，shRNA，基因疗法，埃及伊蚊，冈比亚按蚊，蚊子，生物制药，生物工程。
图形摘要
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