Login Register Cart 0
Generic placeholder image

Recent Patents on Biotechnology

Editor-in-Chief

ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

The Effect of Alpha-Tocopherol on Morphine Tolerance-induced Expression of c-fos Proto-oncogene from a Biotechnological Perspective

Author(s): Soraya Mehrabi, Farinaz Nasirinezhad*, Mahmood Barati, Nahid Abutaleb, Shirin Barati, Bahram Teymoory Dereshky, Naser Amini, Peiman Brouki Milan, Ahmad Jahanmahin, Arash Sarveazad, Ali Samadikuchaksaraei and Masoud Mozafari

Volume 13, Issue 2, 2019

Page: [137 - 148] Pages: 12

DOI: 10.2174/1872208312666181120105333

Price: $65

Abstract

Background: The increase of oxidant compounds is the most well-known reasons for the tolerance to the analgesic properties of Morphine. Additionally, the production of proxy-nitrite impairs receptors, proteins and enzymes involved in the signaling pathways of analgesia, apoptosis and necrosis. Also, we revised all patents relating to opioid tolerance control methods.

Objective: The aim of this study was to assess the effects of Alpha-tocopherol as an anti-oxidant agent to reduce Morphine tolerance.

Method: Forty male rats randomly divided into four groups. 10 mg/kg of morphine was injected subcutaneously to create the desired level of tolerance. After modeling, 70 mg/kg Alpha- Tocopherol was injected intraperitoneal. Also, the hot plate recorded pain threshold alterations was used to evaluate the behavioral test. All tissue samples were extracted from the spinal cord, thalamus and frontal cortex for molecular and gene expression evaluations. Also, the effect of Alpha- Tocopherol on the apoptosis and necrosis parameters was analyzed using nissl staining and tunel test.

Results: The time latency results showed that there were no significant differences in the different days in groups treated with Morphine plus Alpha-Tocopherol. However, our data highlighted that the pain threshold and their time latency in respond to it had substantially increased in comparison with the control group. Furthermore, we found that the Alpha-Tocopherol obviously decreased c-fos gene expression, especially in the spinal cord.

Conclusion: Thus, co-administration of Alpha-Tocopherol with Morphine can decrease the adverse effects of nitrite proxy, which is released due to repeated injections of Morphine.

Keywords: Alpha-tocopherol, morphine tolerance, C-fos, peroxynitrite, proto-oncogene, morphine.

« Previous Next »
Graphical Abstract

[1]
Salvemini D, Neumann WL. Peroxynitrite: a strategic linchpin of opioid analgesic tolerance. Trends Pharmacol Sci 2009; 30(4): 194-202.
[2]
Ghavimi H, Darvishi S, Ghanbarzadeh S. Attenuation of morphine-induced tolerance and dependence by pretreatment with cerebrolysin in male rats. Drug Res 2018; 68(01): 33-7.
[3]
Deng X-T, Han Y, Liu W-T, Song X-J. B vitamins potentiate acute morphine antinociception and attenuate the development of tolerance to chronic morphine in mice. Pain Med 2017; 18(10): 1961-74.
[4]
Zanelli S, Ashraf Q, Mishra O. Nitration is a mechanism of regulation of the NMDA receptor function during hypoxia. Neuroscience 2002; 112(4): 869-77.
[5]
Waters KA, Machaalani R. NMDA receptors in the developing brain and effects of noxious insults. Neurosignals 2004; 13(4): 162-74.
[6]
Mennerick S, Shen W, Xu W, et al. Substrate turnover by transporters curtails synaptic glutamate transients. J Neurosci 1999; 19(21): 9242-51.
[7]
Chadwick DJ, Goode JA. Pathological pain: From molecular to clinical aspects. Hoboken, NJ: John Wiley & Sons 2005.
[8]
Mao J, Sung B, Ji R-R, Lim G. Chronic morphine induces downregulation of spinal glutamate transporters: implications in morphine tolerance and abnormal pain sensitivity. J Neurosci 2002; 22(18): 8312-23.
[9]
Bharath S, Hsu M, Kaur D, Rajagopalan S, Andersen JK. Glutathione, iron and Parkinson’s disease. Biochem Pharmacol 2002; 64(5-6): 1037-48.
[10]
Harris JA. Using c-fos as a neural marker of pain. Brain Res Bull 1998; 45(1): 1-8.
[11]
Arout CA, Caldwell M, McCloskey DP, Kest B. C-Fos activation in the periaqueductal gray following acute morphine-3β-D-glucuronide or morphine administration. Physiol Behav 2014; 130: 28-33.
[12]
Coggeshall RE. Fos, nociception and the dorsal horn. Prog Neurobiol 2005; 77(5): 299-352.
[13]
Asma HA, Zalina I. C-Fos and its consequences in pain. Malays J Med Sci 2002; 9: 3-8.
[14]
Ramezani S, Vousooghi N, Kapourchali FR, et al. Rolipram potentiates bevacizumab-induced cell death in human glioblastoma stem-like cells. Life Sci 2017; 173: 11-9.
[15]
Milan PB, Lotfibakhshaiesh N, Joghataie M, et al. Accelerated wound healing in a diabetic rat model using decellularized dermal matrix and human umbilical cord perivascular cells. Acta Biomater 2016; 45: 234-46.
[16]
Loo DT. In Situ detection of apoptosis by the TUNEL assay: an overview of techniques.In: Didenko V,EdsDNA Damage detection in situ, ex vivo, and in vivo Methods in Molecular Biology. Totowa, NJ: Springer 2011; pp. 3-13.
[17]
Amini N, Vousooghi N, Soleimani M, et al. A new rat model of neonatal bilirubin encephalopathy (kernicterus). J Pharmacol Toxicol Methods 2017; 84: 44-50.
[18]
Amini N, Vousooghi N, Hadjighassem M, et al. Efficacy of human adipose tissue-derived stem cells on neonatal bilirubin encephalopathy in rats. Neurotox Res 2016; 29(4): 514-24.
[19]
Asle-Rousta M, Kolahdooz Z, Oryan S, Ahmadiani A, Dargahi L. FTY720 (fingolimod) attenuates beta-amyloid peptide (Aβ42)-induced impairment of spatial learning and memory in rats. J Mol Neurosci 2013; 50(3): 524-32.
[20]
Ramezani S, Hadjighassem M, Vousooghi N, et al. The role of protein kinase b signaling pathway in anti-cancer effect of rolipram on glioblastoma multiforme: An in vitro study. Basic Clin Neurosci 2017; 8(4): 325.
[21]
Mohammadi A, Maleki-Jamshid A, Milan P, Ebrahimzadeh K, Faghihi F, Joghataei M. Intrahippocampal transplantation of undifferentiated human chorionic-derived mesenchymal stem cells does not improve learning and memory in the rat model of sporadic alzheimer disease Curr Stem Cell Res Ther 2018: 2018: [Epub ahead of print].
[22]
Singh RP, Sharad S, Kapur S. Free radicals and oxidative stress in neurodegenerative diseases: relevance of dietary antioxidants. J Indian Acad Clin Med 2004; 5(3): 218-25.
[23]
Fattman CL, Schaefer LM, Oury TD. Extracellular superoxide dismutase in biology and medicine. Free Radic Biol Med 2003; 35(3): 236-56.
[24]
Sagen J. Preventing opiate tolerance by cellular implantation.US5762925A 1994.
[25]
Cobley JN, McHardy H, Morton JP, Nikolaidis MG, Close GL. Influence of vitamin C and vitamin E on redox signaling: Implications for exercise adaptations. Free Radic Biol Med 2015; 84: 65-76.
[26]
Engin KN. Alpha-tocopherol: looking beyond an antioxidant. Mol Vis 2009; 15: 855.
[27]
Malfroy B, Baudry C. Synthetic catalytic free radical scavengers useful as antioxidants for prevention and therapy of disease. US5403834A, 1992.
[28]
Le Guen S, Catheline G, Besson J-M. Development of tolerance to the antinociceptive effect of systemic morphine at the lumbar spinal cord level: a c-Fos study in the rat. Brain Res 1998; 813(1): 128-38.
[29]
Kim HK, Kim JH, Gao X, Zhou J-L, Lee I, Chung K, et al. Analgesic effect of vitamin E is mediated by reducing central sensitization in neuropathic pain. Pain 2006; 122(1-2): 53-62.
[30]
Vatassery GT, Smith WE, Quach HT. α-Tocopherol in rat brain subcellular fractions is oxidized rapidly during incubations with low concentrations of peroxynitrite. J Nutr 1998; 128(2): 152-7.
[31]
Mansouri MT, Naghizadeh B, Ghorbanzadeh B. Ellagic acid enhances morphine analgesia and attenuates the development of morphine tolerance and dependence in mice. Eur J Pharmacol 2014; 741: 272-80.
[32]
Oshlack B, Colucci R, Wright C, Breder C. Pharmaceutical formulation containing opioid agonist, opioid antagonist and bittering agent. US20030124185A1, 2001.
[33]
Habib T, Begum S, Ali T, Imtiaz M, Masood S. antinociceptive and anti-inflammatory effects of combined administration of a-tocopherol and morphine in acetic acid induced writhing test. Anwer Khan Mod Med Coll J 2017; 7(2): 20-4.
[34]
Miranda HF, Noriega V, Zanetta P, et al. Isobolographic analysis of the opioid-opioid interactions in a tonic and a phasic mouse model of induced nociceptive pain. J Biomed Sci 2014; 21(1): 62.
[35]
Bravo D, Maturana C, Pelissier T, Hernandez A, Constandil L. Interactions of pannexin 1 with NMDA and P2X7 receptors in central nervous system pathologies: possible role on chronic pain. Pharmacol Res 2015; 101: 86-93.
[36]
Hassanzadeh K, Habibi-asl B, Farajnia S, Roshangar L. Minocycline prevents morphine-induced apoptosis in rat cerebral cortex and lumbar spinal cord: a possible mechanism for attenuating morphine tolerance. Neurotox Res 2011; 19(4): 649-59.

Rights & Permissions Print Cite
Article Metrics
82
5
2
2
© 2024 Bentham Science Publishers | Privacy Policy