三倍体鲫鱼对镉诱导的氧化和内质网应激的增强抵抗力

Title:Enhanced Resistance of Triploid Crucian Carp to Cadmium-induced Oxidative and Endoplasmic Reticulum Stresses

Volume: 18 Issue: 6

关键词: 镉，三倍体鲫鱼，ER应激，氧化应激，IRE-1，PERK。

摘要: Background: Cadmium is a major heavy metal pollutant. Even at low concentrations in waste water Cadmium can accumulate in algae and sediments, and it is absorbed by both plants and aquatic animals, like shellfish and fish. Triploid crucian carp is an important economic fish, and have been farmed on a large scale in China.

Objective: to explore the molecular mechanisms underlying Cadmium stress of triploid crucian carp.

Method: we applied following experimental method to conduct research: transcriptomes analysis, histological observation, Quantitative real-time PCR and enzyme activity analysis et al.

Results: in the present study, we obtained following important results: 1) Under Cd stress, the mortality and abnormality rates in triploid crucian carp were lower than those of diploid strains and the effects of Cadmium treatment on the liver were revealed by histological observation; 2) the liver transcriptomes of triploid crucian carp were obtained and the data was analyzed; 3) 14 genes related to algae and sediments stress were screened from transcriptome data, and the expression of these genes was verified by Quantitative real-time PCR. The results were consistent with the gene results in transcriptome data; 4) Quantitative real-time PCR and enzyme activity analysis all confirmed triploid crucian carp resistance to Cadmium stress was regulated by both oxidative stress and ER stress responses; 5) We found that IRE-1 and PERK, not ATF- 6, were involved in the enhance Cadmium resistance of triploid crucian carp.

Conclusion: these studies demonstrate that triploid crucian carp have strong resistance to Cadmium.

Cite this article as:

Enhanced Resistance of Triploid Crucian Carp to Cadmium-induced Oxidative and Endoplasmic Reticulum Stresses, Current Molecular Medicine 2018; 18 (6) . https://dx.doi.org/10.2174/1566524018666181113105018