MiR-19b Functions as a Potential Protector in Experimental Autoimmune Encephalomyelitis

Title:MiR-19b Functions as a Potential Protector in Experimental Autoimmune Encephalomyelitis

Volume: 18 Issue: 5

Author(s): B. Hu, Z.L. Hu, Q.M. Zeng, B. Xiao and H. Yang*

Affiliation:

• Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008,China

Keywords: MiR-19b, experimental autoimmune encephalomyelitis, oligodendrocyte, PTEN, agomiR-19b, MBP, CNP.

Abstract: Background: MicroRNA-19b (miR-19b) is essential in determining oligodendroglia proliferation. Phosphatase and tensin homologue on chromosome 10 (PTEN) is considered the target of miR-19b and participates in oligodendrocyte differentiation and proliferation.

Methods: Murine EAE was induced by myelin oligodendrocyte glycoprotein (MOG35– 55). For EAE reversal, artificially synthesized agomiR-19b was intravenous injected after immunization.

Results: We found that the expression of miR-19b is significantly reduced in an experimental autoimmune encephalomyelitis (EAE) mouse model. This downregulation, which is associated with the neurological scores, can be dramatically ameliorated by agomiR-19b. Our results show that agomiR-19b increases the expression of myelin basic protein (MBP) and cyclic nucleotide phosphodiesterase (CNP), which are regularly utilized as molecular markers of oligodendrocytes. Furthermore, our study also revealed that miR-19b probably affects the expression of PTEN in the EAE model.

Conclusion: These results indicate that the restoration of miR-19b probably exerts its therapeutic effect by affecting PTEN in the pathogenesis of EAE.

Cite this article as:

Hu B. , Hu Z.L., Zeng Q.M., Xiao B. and Yang H. *, MiR-19b Functions as a Potential Protector in Experimental Autoimmune Encephalomyelitis, Current Molecular Medicine 2018; 18 (5) . https://dx.doi.org/10.2174/1566524018666181004123716