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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

HAMPT，一种用于癌症转移化学预防的新型四联药物组合：从假设到概念验证

卷 19, 期 4, 2019

页: [296 - 303] 页: 8

弟呕挨: 10.2174/1568009618666181001102557

价格: $65

摘要

背景：高活性转移预防治疗（HAMPT）是一种由米非司酮，阿司匹林，赖氨酸和强力霉素组成的四聚体药物组合。 目的：在此前的研究基础上，我们进一步证明HAMPT可以有效，安全地预防结肠癌的转移。 方法：专门用于协同控制关键癌转移途径。 HAMPT的剂量设计低于药学推荐剂量，因此亚健康癌症幸存者可以安全地长期服用HAMPT进行转移化学预防。 结果：HAMPT在其有效浓度范围内（1-50μg/ mL）对结肠癌细胞HT-29和CT-26无细胞毒性，但显着抑制这些结肠癌细胞对人脐带血管内皮细胞的粘附和侵袭（ HUVECs）和Matrigel。 HAMPT表现出良好的粘附抑制率，表明其主要通过抑制癌细胞与HUVEC的粘附而不是杀死癌细胞起作用。在低浓度下，HAMPT还抑制癌细胞迁移。流式细胞术分析显示HAMPT对细胞周期没有显着影响，但抑制IL-1β诱导的HUVEC的E-选择蛋白和HT-29的唾液酸-Lewis X的表达。体内实验表明，HAMPT以剂量依赖性方式抑制CT-26细胞向小鼠肺的转移。在小鼠模型中，HAMPT在预防转移方面显示出优于其他组合的优势。 结论：本研究表明HAMPT是一种新型四联药物组合，可安全有效地预防癌症转移。

关键词: 结肠癌转移，药物组合，循环肿瘤细胞，细胞粘附分子HAMPT，化学预防，四联药物组合。

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