Login Register Cart 0
Generic placeholder image

Current Analytical Chemistry

Editor-in-Chief

ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Simultaneous Determination of Four Iridoid Glycosides from Paederia Scandens in Rat Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study

Author(s): Yanxiang Xie, Ergang Jiang, Tianming Dai and Renke Dai*

Volume 16, Issue 3, 2020

Page: [298 - 307] Pages: 10

DOI: 10.2174/1573411014666180907152359

Price: $65

Abstract

Background: Paederia scandens (Lour.) Merrill, belonging to the Rubiaceae family, was one of the Traditional Chinese Medicines that has been generally used to treat aches and pains, inflammation, bacillary dysentery, piles and rheumatic arthritis. The four iridoid glycosides that Paederosidic acid, Paederoside, Paederosidic acid methyl ester, and Asperuloside from Paederia scandens are considered to be the main bioactive constituents. However, their pharmacokinetics and action mechanisms of these iridoid glycosides still remain unknown. In this study, a method of simultaneous determination of four iridoid glycosides components in rat plasma with LC-MS/MS has been established and successfully applied to a pharmacokinetic study in rats, which would be favourable for further exploration.

Methods: In this study, a sensitive, rapid, accurate bioanalytical method of simultaneous determination of four iridoid glycosides components from Paederia scandens extract in rat plasma with LCMS/ MS has been established. It is easy and rapid for LC-MS/MS to accurately separate and simultaneously quantify the analytes because of its high sensitivity, selectivity and accuracy. This validated method was successfully applied to a pharmacokinetic study in rats with intravenous and oral administrations of Paederia scandens extract.

Results: The resulting data are fully satisfied for the selectivity and the sensitivity. Their lower limit of quantitation are in 0.25-0.5 ng/mL. Intra- and interday precisions were less than 15.0% with accuracy in the range of -15.0% to 15.0%. In the stability measurements including repeated Freeze-thaw, 4 hours at 4°C, 24 hours at 4°C, and 30 days at -80°C, the variations between the measurements and the nominated values of these compounds in plasma were detected to be less than 15.0%. The extraction recoveries of the compounds were from 85.4% to 105.6%. The bioavailability (F) of the four iridoid glycosides were 1.74% to 3.36%.

Conclusion: In summary, a simple, rapid, precise, and sensitive LC-MS/MS method has been established and validated to determine the four iridoid glycosides from Paederia scandens extract in rat plasma, which was successfully applied to pharmacokinetic study in rats. The information obtained from the study will facilitate further exploration on Paederosidic acid, Paederoside, Paederosidic acid methyl ester as well as Asperuloside and these may provide valuable pharmacokinetic reference for human applications.

Keywords: HPLC-MS/MS, iridoid glycosides, Paederia scandens, pharmacokinetics, simultaneous quantification, rat plasma.

« Previous Next »
Graphical Abstract

[1]
Quang, D.N.; Hashimoto, T.; Tanaka, M.; Dung, N.X.; Asakawa, Y. Iridoid glucosides from roots of Vietnamese Paederia scandens. Phytochemistry, 2002, 60(5), 505-514.
[http://dx.doi.org/10.1016/S0031-9422(02)00096-1] [PMID: 12052517]
[2]
Wang, L.; Jiang, Y.; Han, T.; Zheng, C.; Qin, L. A phytochemical, pharmacological and clinical profile of Paederia foetida and P. scandens. Nat. Prod. Commun., 2014, 9(6), 879-886.
[http://dx.doi.org/10.1177/1934578X1400900640] [PMID: 25115105]
[3]
Wu, Z-J.; Wang, J-H.; Fang, D-M.; Zhang, G-L. Analysis of iridoid glucosides from Paederia scandens using HPLC-ESI-MS/MS. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2013, 923-924, 54-64.
[http://dx.doi.org/10.1016/j.jchromb.2013.01.032] [PMID: 23466447]
[4]
He, D.H.; Chen, J.S.; Wang, X.L. A new iridoid glycoside from Paederia scandens. Chin. Chem. Lett., 2010, 21(4), 437.
[http://dx.doi.org/10.1016/j.cclet.2009.12.009]
[5]
Wang, D-M.; Xu, Y-F.; Chen, Z.; Huang, L-F.; Chen, S-L. UPLC/Q-TOF-MS analysis of iridoid glycosides and metabolites in rat plasma after oral administration of Paederia scandens extracts. Chin. J. Nat. Med., 2015, 13(3), 215-221.
[http://dx.doi.org/10.1016/S1875-5364(15)30007-8] [PMID: 25835366]
[6]
Kim, Y.L.; Chin, Y.W.; Kim, J.; Park, J.H. Two new acylated iridoid glucosides from the aerial parts of Paederia scandens. Chem. Pharm. Bull. (Tokyo), 2004, 52(11), 1356-1357.
[http://dx.doi.org/10.1248/cpb.52.1356] [PMID: 15516763]
[7]
Zou, X.; Peng, S.; Liu, X.; Bai, B.; Ding, L. Sulfur-containing iridoid glucosides from Paederia scandens. Fitoterapia, 2006, 77(5), 374-377.
[http://dx.doi.org/10.1016/j.fitote.2006.05.003] [PMID: 16797140]
[8]
Peng, W.; Qiu, X.Q.; Shu, Z.H.; Liu, Q.C.; Hu, M.B.; Han, T.; Rahman, K.; Qin, L.P.; Zheng, C.J. Hepatoprotective activity of total iridoid glycosides isolated from Paederia scandens (lour.) Merr. var. tomentosa. J. Ethnopharmacol., 2015, 174, 317-321.
[http://dx.doi.org/10.1016/j.jep.2015.08.032] [PMID: 26320683]
[9]
Otsuka, H. Two New Iridoid Glucosides from Paederia scandens (Lour.) Merr. var. mairei (Leveille) Hara. Nat. Med., 2002, 56(2), 59.
[10]
Wang, Y.C.; Huang, T.L. Screening of anti-Helicobacter pylori herbs deriving from Taiwanese folk medicinal plants. FEMS Immunol. Med. Microbiol., 2005, 43(2), 295-300.
[http://dx.doi.org/10.1016/j.femsim.2004.09.008] [PMID: 15681161]
[11]
Konoshima, T.; Takasaki, M.; Tokuda, H.; Nishino, H. Cancer chemopreventive activity of an iridoid glycoside, 8-acetylharpagide, from Ajuga decumbens. Cancer Lett., 2000, 157(1), 87-92.
[http://dx.doi.org/10.1016/S0304-3835(00)00479-1] [PMID: 10893446]
[12]
Chen, Y.F.; Li, N.; Jiao, Y.L.; Wei, P.; Zhang, Q.Y.; Rahman, K.; Zheng, H.C.; Qin, L.P. Antinociceptive activity of petroleum ether fraction from the MeOH extracts of Paederia scandens in mice. Phytomedicine, 2008, 15(6-7), 427-436.
[http://dx.doi.org/10.1016/j.phymed.2007.09.023] [PMID: 17976971]
[13]
FDA. 2001.Guidance for industry: bioanalytical methods validation., http://www.fda.gov/downloads/Drugs/GuidanceCompliance RegulatoryInformation/Guidances/UCM070107.pdf pdf (Accessed May 2001).
[14]
Jiang, E.; Deng, J.; Guo, J.; Li, Y.; Tan, L.; Cheng, H.; Dai, R. Determination of A Novel Selective B-Raf V600E Inhibitor (LXK4) in Dog Plasma by HPLC-MS/MS and its Application in a Pharmacokinetic Study. Chromatographia, 2016, 80(1), 1.
[15]
Yang, G.; Lu, W.; Pan, M.; Zhang, C.; Zhou, Y.; Hu, P.; Hu, M.; Song, G. An LC-MS/MS method for simultaneous determination of nine steroidal saponins from Paris polyphylla var. in rat plasma and its application to pharmacokinetic study. J. Pharm. Biomed. Anal., 2017, 145, 675-681.
[http://dx.doi.org/10.1016/j.jpba.2017.07.052] [PMID: 28802242]
[16]
Sun, R.; Zeng, M.; Du, T.; Li, L.; Yang, G.; Hu, M.; Gao, S. Simultaneous determinations of 17 marker compounds in Xiao-Chai-Hu-Tang by LC-MS/MS: Application to its pharmacokinetic studies in mice. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2015, 1003, 12-21.
[http://dx.doi.org/10.1016/j.jchromb.2015.09.004] [PMID: 26397748]
[17]
Yu, Y.; Li, Q.; Bi, K.; Xie, P.; Yang, G.; Chen, X. A sensitive liquid chromatography-mass spectrometry method for simultaneous determination of alisol A and alisol A 24-acetate from Alisma orientale (Sam.) Juz. in rat plasma. Anal. Bioanal. Chem., 2011, 399(3), 1363-1369.
[http://dx.doi.org/10.1007/s00216-010-4426-9] [PMID: 21107819]
[18]
Wu, W.J.; Yan, R.; Li, T.; Li, Y.P.; Zhou, R.N.; Wang, Y.T. Pharmacokinetic alterations of rhubarb anthraquinones in experimental colitis induced by dextran sulfate sodium in the rat. J. Ethnopharmacol., 2017, 198, 600-607.
[http://dx.doi.org/10.1016/j.jep.2017.01.049] [PMID: 28214059]
[19]
Lei, T.; Zhang, D.; Guo, K.; Li, M.; Lv, C.; Wang, J.; Jia, L.; Lu, J. Validated UPLC-MS/MS method for simultaneous quantification of eight saikosaponins in rat plasma: Application to a comparative pharmacokinetic study in depression rats after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2017, 1060, 231-239.
[http://dx.doi.org/10.1016/j.jchromb.2017.06.007] [PMID: 28628838]
[20]
Zhou, Q.L.; Zhu, D.N.; Yang, Y.F.; Xu, W.; Yang, X.W. Simultaneous quantification of twenty-one ginsenosides and their three aglycones in rat plasma by a developed UFLC-MS/MS assay: Application to a pharmacokinetic study of red ginseng. J. Pharm. Biomed. Anal., 2017, 137, 1-12.
[http://dx.doi.org/10.1016/j.jpba.2017.01.009] [PMID: 28086165]
[21]
Xing, J.; Chen, X.; Zhong, D. Absorption and enterohepatic circulation of baicalin in rats. Life Sci., 2005, 78(2), 140-146.
[http://dx.doi.org/10.1016/j.lfs.2005.04.072] [PMID: 16107266]
[22]
Ma, Y.; Li, P.; Chen, D.; Fang, T.; Li, H.; Su, W. LC/MS/MS quantitation assay for pharmacokinetics of naringenin and double peaks phenomenon in rats plasma. Int. J. Pharm., 2006, 307(2), 292-299.
[http://dx.doi.org/10.1016/j.ijpharm.2005.10.018] [PMID: 16289985]
[23]
Xu, W.; Li, X.; Lin, N.; Zhang, X.; Huang, X.; Wu, T.; Tai, Y.; Chen, S.; Wu, C.H.; Huang, M.; Wu, S. Pharmacokinetics and tissue distribution of five major triterpenoids after oral administration of Rhizoma Alismatis extract to rats using ultra high-performance liquid chromatography-tandem mass spectrometry. J. Pharm. Biomed. Anal., 2017, 146, 314-323.
[http://dx.doi.org/10.1016/j.jpba.2017.09.009] [PMID: 28910706]

Rights & Permissions Print Cite
Article Metrics
8
© 2024 Bentham Science Publishers | Privacy Policy