Antioxidant, Antimicrobial and the Phenolic Content of Infusion, Decoction and Methanolic Extracts of Thyme and Rosmarinus Species

Title:Antioxidant, Antimicrobial and the Phenolic Content of Infusion, Decoction and Methanolic Extracts of Thyme and Rosmarinus Species

Volume: 19 Issue: 7

Author(s): Amira Zairi*, Sahar Nouir, Naceur M`hamdi, Marwa Bennani, Ines Bergaoui, Ali Mtiraoui, Maher Chaouachi and Mounir Trabelsi

Affiliation:

• Laboratory of Biochemistry, Faculty of Medicine, University of Sousse, Sousse,Tunisia

Keywords: Plant extracts, phytochemicals, tlc, antimicrobials, antioxidants, cytotoxicity, aqueous extracts, decoction, infusion.

Abstract: Background: The plant species Rosmarinus officinalis (RO), Thymus algeriensis (TA) and Thymus capitatus (TC) are widely used in traditional medicine in Tunisia. Their bioactivities have been reported before and particularly referred to their essential oils. The main objective of this work was to assess the phytochemical composition, the antioxidant activity, the antibacterial, antifungal, and cytotoxic potential of these 3 plants.

Method: The High Performance Liquid Chromatography (HPLC), chemical tests and spectrophotometric methods were used for screening, quantification of phytochemicals and for antioxidant activities. Extracts were evaluated for antibacterial potential by the microdilution method. Antifungal activities were tested using the Poisoned food method against: Aspergillus niger and Aspergillus flavus. The cytotoxic potential of the plant extracts was checked using HCT 116 cultures.

Results: Results revealed that aqueous extracts are not toxic compared to the methanolic extracts. Phenolic compounds were detected and these extracts showed excellent antioxidant activity presenting dose-dependent relationship. For antibacterial potential, all tested strains are more sensitive to Thymus extracts than Rosmarinus extracts. However, for antifungal activities, only Rosmarinus extracts inhibited mycelial growth. HPLC analysis allowed the identification of ten compounds with the abundance of gallic acid.

Conclusion: This study showed important bioactivities (antioxidant, antimicrobial, and safety potential) of the plant species RO, TA and TC used in traditional medicine.

Cite this article as:

Zairi Amira *, Nouir Sahar , M`hamdi Naceur , Bennani Marwa , Bergaoui Ines , Mtiraoui Ali, Chaouachi Maher and Trabelsi Mounir , Antioxidant, Antimicrobial and the Phenolic Content of Infusion, Decoction and Methanolic Extracts of Thyme and Rosmarinus Species, Current Pharmaceutical Biotechnology 2018; 19 (7) . https://dx.doi.org/10.2174/1389201019666180817141512