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Current Analytical Chemistry

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ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

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Research Article

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

Author(s): Ying Wei, Yongqiao Liu, Yifan Hele, Weiwei Sun, Yang Wang, Tunhai Xu* and Tonghua Liu

Volume 15, Issue 1, 2019

Page: [21 - 33] Pages: 13

DOI: 10.2174/1573411014666180730113804

Price: $65

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Abstract

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination.

Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination.

Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed.

Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability.

Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

Keywords: Content determination, Gentianella acuta (Michx.) Hulten, HPLC analysis, isolation, medicinal plants, traditional medicine.

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[1]
Urbain, A.; Marston, A.; Batsuren, D.; Purev, O.; Hostettmann, K. Preparative isolation of closely-related xanthones from gentianella amarella ssp. acuta by high-speed countercurrent chromatography. Phytochem. Anal., 2008, 19(6), 514-519.
[2]
Lv, L.J.; Li, M.H. Terpenoids, flavonoids and xanthones from Gentianella acuta, (Gentianaceae). Biochem. Syst. Ecol., 2009, 37(4), 497-500.
[3]
Li, M.H.; Jin, M.; Zhang, H.T. Study on chemical constituents of Gentianella acuta. J. Baotou. Med. College, 2010, 27, 13-14.
[4]
Wu, H.J.; Li, M.H.; Zhang, H.T. Effect of bellidifolin on electrocardiogram in anesthetized rats. J. Baotou. Med. Coll, 2011, 27, 22.
[5]
Tian, L.Y.; Bai, X.; Chen, X.H. Anti-diabetic effect of methylswertianin and bellidifolin from Swertiapunicea Hemsl. and its potential mechanism. Phytomedicine, 2010, 17, 533-539.
[6]
Song, H.J.; Zhang, J.H.; Yan, P. Experimental study on the protective effect of bellidifolin on ischemic brain injury. J. Dalian Med. Univ, 2007, 29, 110-112.
[7]
Pradeep, P.S. Ambika, Chauhan, S.M. Activity-guided isolation of antioxidant xanthones from Swertiachirayita (Roxb.) H. Karsten (Gentianaceae). Nat. Prod. Res., 2012, 26, 1682-1686.
[8]
Liu, H.Y.; He, H.P.; Yang, X.W.; Chen, M.W.; Hao, X.J. Chemical constituents of the flowers of Helichrysum bracteatum. Nat. Prod. Res. Develop, 2007, 19, 423-426. [in Chinese].
[9]
Kojima, K.; Ondognij, P. Davgiin. K.; Ogihara, Y.; Xanthones from Gentiana acuta. Nat. Med., 1998, 52, 87.
[10]
He, L.W.; Meng, Z.M. Studies on Constituents of alternanthera philoxeroides (Mart) Griseb. J. China. Pharma, 1995, 25, 263-267.
[11]
Xu, X.D.; Yang, J-S. Studies on chemical constituents of Swertia pubescens. Chin. Pharm. J, 2005, 40, 57-59.
[12]
Sakamoto, I.; Tanaka, T.; Tanaka, O.; Tomimori, T. Xanthone glucosides of Swertia japonica makino and a related plant: Structure of a new glucoside, isoswertianolin and structure revision of swertianolin and norswertianolin. Chem. Pharm. Bull., 1982, 30, 4088-4091.
[13]
Zhang, Y.J.; Yang, C.R. Chemical studies on gentianella azurea, A tibetan medicinal plant. . Acta. Botanica. Yunnanica, 1994, 6, 401-406.
[14]
Zhu, K.C.; Ma, C.H.; Fan, M.S. Xanthones from Tripterospermum chinese (Migo). Asian J. Chem., 2007, 19, 1739-1742.
[15]
Kojima, K.; Ondognij, P. Davgiin. K.; Ogihara, Y.; Xanthones from gentiana acuta. Nat. Med., 1998, 52, 87.
[16]
Zhou, H.M.; Liu, Y.L.; Gabor, B.; Cordell, G.A. Swertiabisxanthone-I from Swertia Macrosperma. Phytochemistry, 1989, 28(12), 3569-3571.
[17]
Tan, G.S.; Xu, P.S.; Tian, H.Y.; Xu, K. Studies on the chemical constituents of Swertia davidi. Chin. Pharm J, 2000, 35, 441-443.
[18]
Fan, W.Q.; Xiong, M.X.; Ma, Z.; Li, Q.Y. Chemical constituents of alternathera philoxeroides. Chin. Tradit. Herbal Drugs, 2008, 39, 499-502.
[19]
Duan, C.H.; Shi, B.J.; Wu, L.H.; Chou, G.X.; Wang, Z.T. Chemical constituents of gentiana waltonii. Chin. J. Nat. Med., 2007, 5, 417-420.
[20]
Akdemir, Z.Ş.; Tatli, I.I.; Bedir, E.; Khan, I.A. Neolignan and Phenylethanoid Glycosides from Verbascum salviifolium Boiss. Turk. J. Chem., 2004, 28(5), 621-628.
[21]
Wang, H.L.; Geng, C.A.; Zhang, X.M.; Ma, Y.; Chen, J. Chemical constituents of Swertia macrosperma. China. J. Chinese. Mater. Medica, 2010, 35, 3161-3164.
[22]
Kong, D.Y.; Jiang, Y. Yao, Ying. Studies on the Glucoside Constituents of Shenggengzhangyacai (Swertia elongata). Chin. Tradit. Herbal Drugs, 1995, 26, 7-10.
[23]
Gohari, A.R.; Saeidnia, S.; Bayati-Moghadam, M. Lignans and neolignans from Stelleropsis antoninae. DARU-J. Pharm. Sci., 2011, 9, 74-79.
[24]
Tian, L.Y.; Chen, J.C.; Bai, X. Xanthonoids and iridoid glycosides from swertia punicea Hemsl. Nat. Prod. Res. Dev, 2010, 22, 979-983.

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