摘要
目的:甲状腺相关眼病是成人最常见的眼眶免疫学疾病之一,CD4辅助性t(TH)细胞在甲状腺相关眼病发病机制中起重要作用,但调节CD4 t细胞活性的机制尚不清楚,本研究探讨了辅助性T细胞1型(Th1)、Th17和调节性T细胞中T细胞免疫球蛋白结构域和粘液结构域3(Tim-3)的表达。方法:将受试者分为三组:甲状腺相关眼病组、Graves病无眼眶病组(Gd)和健康对照组(HC),每组每例患者均采集外周血标本,检测流式细胞术检测Th1(CD4 IFN-γ)、Th17(CD4 IL-17)、调节性T细胞(CD4 Cd25hicd127lo)和Tim-3蛋白表达的频率,平均荧光强度(MFI)、TIM-3的表达量和TIM-3细胞的百分比。结果:与GD眼眶病组相比,甲状腺相关眼病患者外周血中Th1和Th17细胞频率较高,Tim-3Th1和Th17细胞比例明显低于GD组,TIM-3细胞百分率与Th1细胞频率呈负相关,Th1和Th17细胞表达显著低于正常对照组。T细胞中Tim-3表达较少,各组间差异无显着性(P>0.05)。
关键词: T细胞免疫球蛋白结构域和粘蛋白结构域3,甲状腺相关眼病,Graves病,CD4辅助T细胞,自身免疫性甲状腺疾病,免疫耐受。
Current Molecular Medicine
Title:Decreased Expression of TIM-3 on Th17 Cells Associated with Ophthalmopathy in Patients with Graves’ Disease
Volume: 18 Issue: 2
关键词: T细胞免疫球蛋白结构域和粘蛋白结构域3,甲状腺相关眼病,Graves病,CD4辅助T细胞,自身免疫性甲状腺疾病,免疫耐受。
摘要: Purpose: Thyroid-associated Ophthalmopathy (TAO) is one of the most common orbital immunological diseases in adults. CD4+ helper T (Th) cells play important roles in the pathogenesis of TAO. But the mechanisms regulating CD4+ T cell activity is unclear. This study examines T cell immunoglobulin domain and mucin domain 3 (TIM-3) expression in helper T cell type 1 (Th1), Th17, and regulatory T cells in sufferers of TAO.
Methods: Participants were divided into 3 groups: patients with TAO, patients with Graves’ disease but without orbitopathy (GD), and healthy control patients (HC). Peripheral blood samples were collected for each patient in the designated group. Flow cytometry methods assessed the frequency of Th1 (CD4+IFN-γ+), Th17 (CD4+IL-17+), regulatory T cells (CD4+CD25hiCD127lo), and TIM-3 protein expression. Mean fluorescence intensity (MFI) measured the magnitude of TIM-3 expression and the percentage of TIM-3+ cells for each patient.
Results: Compared to the GD group, TAO patients possessed higher frequencies of Th1 and Th17 cells in peripheral blood samples. The percentage of TIM-3+ Th1 and Th17 cells was significantly lower in the TAO patients than the GD group. Across all patients sampled, TIM-3+ cell percentage negatively correlated with Th1 cell frequency. Th1 and Th17 cells exhibited significantly decreased expression of TIM-3 in TAO patients compared to healthy controls. Regulatory T cells showed little TIM-3 expression and we observed no significant differences in frequency between groups.
Conclusion: These results suggest a role for TIM-3 in the regulation of Th1 and Th17 cells and the pathogenesis of Graves’ ophthalmopathy.
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Cite this article as:
Decreased Expression of TIM-3 on Th17 Cells Associated with Ophthalmopathy in Patients with Graves’ Disease, Current Molecular Medicine 2018; 18 (2) . https://dx.doi.org/10.2174/1566524018666180705105753
DOI https://dx.doi.org/10.2174/1566524018666180705105753 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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