摘要
背景:阿尔茨海默病中星形胶质细胞的改变是众所周知的,反应性星形胶质细胞病是该病的标志之一。最近,数据显示 星形胶质细胞的改变也可能在疾病的早期发生。目的:研究培养乳杆菌的转录变化。 3xTg-AD小鼠幼鼠的TES与对照鼠比较。此外,我们还将这些变化与其他症状性AD小鼠星形胶质细胞中的变化进行了比较。 方法:对3xTg-AD和非转基因小鼠新生幼犬海马原代培养的星形胶质细胞进行全基因组芯片研究。我们使用了跨平台标准化和一个u。 非监督分级聚类算法,以比较我们的结果与其他数据集的培养或刚分离的星形胶质细胞,包括从斑块期APPswe/PS1dE9 AD小鼠。 结果:与非TG星形胶质细胞相比,3xTg-AD中有993个基因表达差异。过度表达的基因本体论术语与钙、细胞通讯、mito有关。 线粒体,转录,核苷酸结合和磷酸化。值得注意的是,在培养的3xTg-AD星形胶质细胞中没有发现与炎症相关的基因。从斑块中分离出星形胶质细胞的比较 年龄APPswe/PS1dE9显示,在原代3xTg-AD星形胶质细胞中,993个基因中有882个基因被选择性地改变,50个基因被共同调控,61个基因被反调控。 在数据集中)。 结论:在AD小鼠模型培养的星形胶质细胞中,转录变化与模仿疾病晚期的模型不同。
关键词: 全基因组微阵列,3xTg-AD小鼠,培养的星形胶质细胞,跨平台正常化,早期阿尔茨海默病,FAD突变。
Current Alzheimer Research
Title:Transcriptional Remodeling in Primary Hippocampal Astrocytes from an Alzheimer’s Disease Mouse Model
Volume: 15 Issue: 11
关键词: 全基因组微阵列,3xTg-AD小鼠,培养的星形胶质细胞,跨平台正常化,早期阿尔茨海默病,FAD突变。
摘要: Background: It is well known that alterations in astrocytes occur in Alzheimer’s disease and reactive astrogliosis is one of the hallmarks of the disease. Recently, data has emerged that suggests that alterations in astrocytes may also occur early in the pathogenesis of the disease.
Objective: The aim of present work was to characterize the transcriptional alterations occurring in cultured astrocytes from 3xTg-AD mouse pups compared to control non-transgenic mice. Furthermore, we also compared these changes to those reported by others in astrocytes from symptomatic AD mice.
Method: We conducted a whole-genome microarray study on primary cultured astrocytes from the hippocampus of 3xTg-AD and non-transgenic mouse newborn pups. We used cross-platform normalization and an unsupervised hierarchical clustering algorithm to compare our results with other datasets of cultured or freshly isolated astrocytes, including those isolated from plaque-stage APPswe/PS1dE9 AD mice.
Results: We found a set of 993 genes differentially expressed in 3xTg-AD as compared with non-Tg astrocytes. Over-represented gene ontology terms were related to calcium, cell-cell communication, mitochondria, transcription, nucleotide binding and phosphorylation. Of note, no genes related to inflammation were found in cultured 3xTg-AD astrocytes. Comparison with astrocytes isolated from plaque stage APPswe/PS1dE9 showed that 882 out of 993 genes were selectively changed in primary 3xTg-AD astrocytes while 50 genes were co-regulated and 61 were anti-regulated (regulated in the opposite direction in the datasets).
Conclusion: Our data show that in cultured astrocytes from an AD mouse model, transcriptional changes occur and are different from those reported in models mimicking later stages of the disease.
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Cite this article as:
Transcriptional Remodeling in Primary Hippocampal Astrocytes from an Alzheimer’s Disease Mouse Model, Current Alzheimer Research 2018; 15 (11) . https://dx.doi.org/10.2174/1567205015666180613113924
DOI https://dx.doi.org/10.2174/1567205015666180613113924 |
Print ISSN 1567-2050 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5828 |
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