Abstract
Background: The effect of milking menaquinone-7 (MK-7) with biocompatible organic solvents on MK-7 production in B. subtilis fermentation was studied. Periodic milking of MK-7 from the fermentation medium by n-hexane significantly enhanced the total MK-7 production ~ 1.7 fold at the end of 72 hours of fermentation (p < 0.05) as compared to the control medium.
Methods: Milking of MK-7 with a mixture of n-hexane phase modified with n-butanol was also explored. Although milking of MK-7 by a mixture of n-hexane and n-butanol (1:2, v/v), was found to be appropriate in terms of high extraction capacity, no significant increase in total MK-7 concentration was observed. Biocompatibility between the extraction solvents and B. subtilis was also examined.
Results and Conclusion: The results indicated that the mixture of n-hexane and n-butanol exhibited some detrimental effects. However, n-hexane alone exhibited delayed toxicity starting at 84 hours of periodic milking and could, therefore, be considered as the most promising organic solvent for milking MK-7 in B. subtilis fermentation while enhancing the productivity of the system.
Keywords: MK-7, B. subtilis, milking, fermentation, viability, n-hexane, n-butanol.
Graphical Abstract
Current Pharmaceutical Biotechnology
Title:High Level of Menaquinone-7 Production by Milking Menaquinone-7 with Biocompatible Organic Solvents
Volume: 19 Issue: 3
Author(s): Dinali Ranmadugala, Alireza Ebrahiminezhad*, Merilyn Manley-Harris, Younes Ghasemi and Aydin Berenjian*
Affiliation:
- Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz,Iran
- Faculty of Science and Engineering, The University of Waikato, Hamilton,New Zealand
Keywords: MK-7, B. subtilis, milking, fermentation, viability, n-hexane, n-butanol.
Abstract: Background: The effect of milking menaquinone-7 (MK-7) with biocompatible organic solvents on MK-7 production in B. subtilis fermentation was studied. Periodic milking of MK-7 from the fermentation medium by n-hexane significantly enhanced the total MK-7 production ~ 1.7 fold at the end of 72 hours of fermentation (p < 0.05) as compared to the control medium.
Methods: Milking of MK-7 with a mixture of n-hexane phase modified with n-butanol was also explored. Although milking of MK-7 by a mixture of n-hexane and n-butanol (1:2, v/v), was found to be appropriate in terms of high extraction capacity, no significant increase in total MK-7 concentration was observed. Biocompatibility between the extraction solvents and B. subtilis was also examined.
Results and Conclusion: The results indicated that the mixture of n-hexane and n-butanol exhibited some detrimental effects. However, n-hexane alone exhibited delayed toxicity starting at 84 hours of periodic milking and could, therefore, be considered as the most promising organic solvent for milking MK-7 in B. subtilis fermentation while enhancing the productivity of the system.
Export Options
About this article
Cite this article as:
Ranmadugala Dinali , Ebrahiminezhad Alireza *, Manley-Harris Merilyn , Ghasemi Younes and Berenjian Aydin *, High Level of Menaquinone-7 Production by Milking Menaquinone-7 with Biocompatible Organic Solvents, Current Pharmaceutical Biotechnology 2018; 19 (3) . https://dx.doi.org/10.2174/1389201019666180515115534
DOI https://dx.doi.org/10.2174/1389201019666180515115534 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers