Progress in One-pot Bioconversion of Cephalosporin C to 7-Aminocephalosporanic Acid

Title:Progress in One-pot Bioconversion of Cephalosporin C to 7-Aminocephalosporanic Acid

Volume: 19 Issue: 1

Author(s): Qiang Tan*, Jie Qiu, Xiang Luo, Yewang Zhang, Yuting Liu, Yuquan Chen, Jing Yuan and Wei Liao

Affiliation:

• Institute of Pharmacy, Guangxi University of Chinese Medicine, Guangxi, 530001,China

Keywords: One-pot, bioconversion, enzymatic process, cephalosporin C, 7-aminocephalosporanic acid, acylase.

Abstract: Background: Cephalosporins are the most widely used semisynthetic antibiotics, which acted on bacterial cell wall (peptidoglycan) synthesis. The key intermediate for fabricating about twothirds of cephalosporins in clinical use is 7-aminocephalosporanic acid (7-ACA), which is derived from chemical or enzymatic deacylation of the natural antibiotic cephalosporin C (CPC). The chemical deacylation process has been replaced by the enzymatic deacylation process because the chemical process required harsh conditions and released toxic waste.

Methods: A two-step enzymatic process that utilized D-amino acid oxidase (DAAO) and 7-β-(4- carboxybutanamido)-cephalosporanic acid acylase (GLA) for two successive reactions has been applied for the conversion of CPC to 7-ACA in an industrial scale.

Results: To simplify the process and lower costs, the one-pot enzymatic processes were developed by the application of the mono-enzymatic process (application of cephalosporin C acylase or the variants of GLA), di-enzymatic process (simultaneous action of DAAO and GLA) or the tri-enzymatic process (simultaneous action of DAAO, GLA and catalase) for direct conversion of CPC to 7-ACA.

Conclusion: Here, we mainly focused on the description of these one-pot enzymatic processes and emphasized on the preparation of the involved biocatalysts.

Cite this article as:

Tan Qiang *, Qiu Jie , Luo Xiang , Zhang Yewang , Liu Yuting , Chen Yuquan , Yuan Jing and Liao Wei , Progress in One-pot Bioconversion of Cephalosporin C to 7-Aminocephalosporanic Acid, Current Pharmaceutical Biotechnology 2018; 19 (1) . https://dx.doi.org/10.2174/1389201019666180509093956