In vitro Studies on Cytotoxic, DNA Protecting, Antibiofilm and Antibacterial Effects of Biogenic Silver Nanoparticles Prepared with Bergenia ciliata Rhizome Extract

Title:In vitro Studies on Cytotoxic, DNA Protecting, Antibiofilm and Antibacterial Effects of Biogenic Silver Nanoparticles Prepared with Bergenia ciliata Rhizome Extract

Volume: 19 Issue: 1

Author(s): Ghazna Zia, Haleema Sadia, Shabnam Nazir, Kiren Ejaz, Shaukat Ali, Ihsan-ul-Haq, Tariq Iqbal, Muhammad A.R. Khan, Abida Raza and Saiqa Andleeb*

Affiliation:

• Microbial Biotechnology Laboratory, Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad, 13100,Pakistan

Keywords: Anti-biofilm assay, cell viability assay, antibacterial assay, brine shrimp assay, UV-vis spectrophotometer, scanning electron microscope, FTIR, protein kinase inhibitor assay.

Abstract: Background: Many health hazardous diseases are caused by clinical pathogens. Drugresistant microbes are one of the major health problems in the world. To overcome the effect of infectious diseases new antimicrobial agent from nature has been explored which is environmentally friendly, less costly and more effective for the development of next-generation drugs. Bergenia ciliata and silver nitrate both have medicinal properties.

Objectives: The aim of the current research was to evaluate the cytotoxic, and antibacterial effect of green synthesized nanoparticles using Bergenia ciliata rhizome against clinical bacterial pathogens.

Methods: Extract of Bergenia ciliata was prepared by maceration technique. Silver nanoparticles were synthesized using Bergenia ciliata rhizome extract. Synthesized silver nanoparticles were confirmed by UV-vis spectrophotometer, Scanning electron microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, DNA protection, brine shrimp lethality effects of synthesized nanoparticles were investigated.

Results: UV-vis spectrophotometer indicated the prelaminar synthesis of silver nanoparticles at 400 nm. The spherical shape of synthesized nanoparticles with 35 nm size was confirmed using SEM. Greatest zone of inhibition (6.0 ± 0.0 mm to 8.3 ± 0.57 mm) was recorded against all tested pathogens compared with the B. ciliata aqueous extract. Anti-biofilm analysis and MTT assay supported the results of the antibacterial activity. Silver nanoparticles protect the DNA degradation.

Conclusion: Green synthesized nanoparticles had potent antibacterial activity and may provide a basis for the development of the new antibacterial drug.

Cite this article as:

Zia Ghazna, Sadia Haleema , Nazir Shabnam , Ejaz Kiren , Ali Shaukat, Ihsan-ul-Haq , Iqbal Tariq , Khan A.R. Muhammad , Raza Abida and Andleeb Saiqa *, In vitro Studies on Cytotoxic, DNA Protecting, Antibiofilm and Antibacterial Effects of Biogenic Silver Nanoparticles Prepared with Bergenia ciliata Rhizome Extract, Current Pharmaceutical Biotechnology 2018; 19 (1) . https://dx.doi.org/10.2174/1389201019666180417160049