摘要
背景:感光细胞死亡是一个关键的视网膜变性疾病的病理学。到目前为止,这个病理过程的分子机制在很大程度上仍不清楚。交叉的附着力c分子(Jam-c)已经被证明能够扮演重要的角色在不同的生物事件。然而,它对视网膜神经细胞的影响是未知的。 目的:确定Jam-c对成年老鼠的眼睛,特别是在视网膜结构、血管和感光细胞,为了探索潜在的眼部疾病的重要目标分子。 方法:Jam-c全球基因敲除小鼠,endothelial-specific neuronal-specific Jam-c条件基因敲除小鼠使用Tie2-Cre和Nestin-Cre小鼠分别被用于这项研究。老鼠的眼睛是收获从不同群体和眼睛的尺寸检查。Cryosections的眼睛和苏木精和伊红染色())和视网膜层的厚度测量。分析了视网膜血管和锥杆光感受器使用isolectin B4,花生凝集素和视紫红质分别标记。在老鼠体内Jam-c击倒的眼睛是由intravitreal注入Jam-c成分。Jam-c表达式在视网膜感觉被实时PCR量化。 结果:全球Jam-c老鼠基因缺失导致小眼睛,减少镜片的直径和虹膜。Jam-c - / -小鼠显示显著变薄的外核层(筒),数量少的感光细胞,视网膜血管异常。重要的是,neuronal-specific Jam-c删除导致相似的表型,而没有明显的缺陷在endothelial-specific Jam-c基因敲除小鼠。此外,Jam-c击倒的成分也减少了辊筒厚度和感光数字。 结论:我们发现Jam-c至关所需的正常大小和视网膜结构。特别是Jam-c扮演重要的角色在维持正常的视网膜厚度、血管和感光号码。因此Jam-c因此可能在各种眼部疾病有重要的作用。
关键词: 交叉的附着力c分子、视网膜光感受器变性
Current Molecular Medicine
Title:Neuronal Expression of Junctional Adhesion Molecule-C is Essential for Retinal Thickness and Photoreceptor Survival
Volume: 17 Issue: 7
关键词: 交叉的附着力c分子、视网膜光感受器变性
摘要: Background: Photoreceptor cell death is a key pathology of retinal degeneration diseases. To date, the molecular mechanisms for this pathological process remain largely unclear. Junctional adhesion molecule-c (Jam-c) has been shown to play important roles in different biological events. However, its effect on retinal neuronal cells is unknown.
Objective: To determine the effect of Jam-c on adult mouse eyes, particularly, on retinal structure, vasculature and photoreceptor cells, in order to explore potential important target molecules for ocular diseases.
Methods: Jam-c global knockout mice, endothelial-specific and neuronal-specific Jam-c conditional knockout mice using Tie2-Cre and Nestin-Cre mice respectively were used in this study. Mouse eyes were harvested from the different groups and eye size examined. Cryosections of the eyes were made and stained with Hematoxylin and Eosin (H&E) and the thicknesses of retinal layers measured. Retinal blood vessels and cone and rod photoreceptors were analyzed using isolectin B4, peanut agglutinin and rhodopsin as markers respectively. In vivo Jam-c knockdown in mouse eyes was performed by intravitreal injection of Jam-c shRNA. Jam-c expression in the retinae was quantified by real-time PCR.
Results: Global Jam-c gene deletion in mice resulted in smaller eyes and decreased the diameters of lens and iris. Jam-c-/- mice display marked thinning of the outer nuclear layer (ONL), less numbers of photoreceptor cells, and abnormal retinal vasculature. Importantly, neuronal-specific Jam-c deletion led to similar phenotype, whereas no obvious defect was observed in endothelial-specific Jam-c knockout mice. Moreover, Jam-c knockdown by shRNA also decreased ONL thickness and photoreceptor numbers.
Conclusion: We found that Jam-c is critically required for the normal size and retinal structure. Particularly, Jam-c plays important roles in maintaining the normal retinal thickness, vasculature and photoreceptor numbers. Jam-c thus may therefore have important roles in various ocular diseases.
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Cite this article as:
Neuronal Expression of Junctional Adhesion Molecule-C is Essential for Retinal Thickness and Photoreceptor Survival, Current Molecular Medicine 2017; 17 (7) . https://dx.doi.org/10.2174/1566524018666180212144500
DOI https://dx.doi.org/10.2174/1566524018666180212144500 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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