Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know

Title:Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know

Volume: 24 Issue: 6

Author(s): Seyed Mohammad Gheibi Hayat, Najmeh Farahani, Behrouz Golichenari and Amirhossein Sahebkar*

Affiliation:

• School of Pharmacy, Mashhad University of Medical Sciences, Mashhad,Iran

Keywords: Escherichia coli, cloning, recombinant protein, vector, host, genome.

Abstract: Background: Host, vector, and culture conditions (including cultivation media) are considered among the three main elements contributing to a successful production of recombinant proteins. Accordingly, one of the most common hosts to produce recombinant therapeutic proteins is Escherichia coli.

Methodology: A comprehensive literature review was performed to identify important factors affecting production of recombinant proteins in Escherichia coli.

Results: Escherichia coli is taken into account as the easiest, quickest, and cheapest host with a fully known genome. Thus, numerous modifications have been carried out on Escherichia coli to optimize it as a good candidate for protein expression and; as a result, several engineered strains of Escherichia coli have been designed. In general; host strain, vector, and cultivation parameters are recognized as crucial ones determining success of recombinant protein expression in Escherichia coli. In this review, the role of host, vector, and culture conditions along with current pros and cons of different types of these factors leading to success of recombinant protein expression in Escherichia coli were discussed.

Conclusion: Successful protein expression in Escherichia coli necessitates a broad knowledge about physicochemical properties of recombinant proteins, selection among common strains of Escherichia coli and vectors, as well as factors related to media including time, temperature, and inducer.

Cite this article as:

Hayat Mohammad Gheibi Seyed, Farahani Najmeh , Golichenari Behrouz and Sahebkar Amirhossein*, Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know, Current Pharmaceutical Design 2018; 24 (6) . https://dx.doi.org/10.2174/1381612824666180131121940