Recent Advances of Butelase 1 in Peptide/Protein Ligations

Title:Recent Advances of Butelase 1 in Peptide/Protein Ligations

Volume: 22 Issue: 8

Author(s): Ye Guo*, Jia-Xing Wang, Hong-Li Zhang and Xiao-Wen Fan

Affiliation:

• Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia 014060,China

Keywords: Chemoselective ligation reaction, peptide ligase, butelase 1, peptide/protein drugs, cyclic peptides, protein modification.

Abstract: Chemoselective ligation reactions provide facile access to various peptides and proteins including cyclic peptides, posttranslationally modified proteins, and peptide/ protein probes. Recently, peptide-ligase-based chemo-enzymatic methods have emerged as promising alternatives to chemical approaches. Ligation mediated by the newly discovered butelase 1 represents one of the state-of-the-art chemo-enzymatic strategies for its high catalytic efficiency and unique substrate specificity. The butelasemediated ligation can be carried out in a semisynthetic manner, in which chemically synthesized peptides and expressed proteins are used as substrates, rendering it suitable for various applications including site-specific modification of proteins and the preparation of thioesters. In this review, we summarize the recent studies and applications of butelase 1.

Cite this article as:

Guo Ye *, Wang Jia-Xing, Zhang Hong-Li and Fan Xiao-Wen, Recent Advances of Butelase 1 in Peptide/Protein Ligations, Current Organic Chemistry 2018; 22 (8) . https://dx.doi.org/10.2174/1385272822666180122152951