光敏剂产生活性氧及其对蛋白质的作用方式

Title:Generation of Reactive Oxygen Species by Photosensitizers and their Modes of Action on Proteins

Volume: 25 Issue: 40

关键词: 单峰氧，光敏剂，氨基酸氧化，乙酰胆碱酯酶，癌症，药物靶向，肽激素

摘要: In this review, we first survey the mechanisms underlying the chemical modification of amino acid residues in proteins by singlet oxygen elicited by photosensitizers. Singlet oxygen has the capacity to cause widespread chemical damage to cellular proteins. Its use in photodynamic therapy of tumors thus requires the development of methodologies for specific addressing of the photosensitizer to malignant cells while sparing normal tissue. We describe three targeting paradigms for achieving this objective. The first involves the use of a photosensitizer with a high affinity for its target protein; in this case, the photosensitizer is methylene blue for acetylcholinesterase. The second paradigm involves the use of the hydrophobic photosensitizer hypericin, which has the capacity to interact selectively with partially unfolded forms of proteins, including nascent species in rapidly dividing or virus-infected and cancer cells, acting preferentially at membrane interfaces. In this case, partially unfolded molten globule species of acetylcholinesterase serve as the model system. In the third paradigm, the photodynamic approach takes advantage of a general approach in ‘state-of-the-art’ chemotherapy, by coupling the photosensitizer emodin to a specific peptide hormone, GnRH, which recognizes malignant cells via specific GnRH receptors on their surface.

Cite this article as:

Generation of Reactive Oxygen Species by Photosensitizers and their Modes of Action on Proteins, Current Medicinal Chemistry 2018; 25 (40) . https://dx.doi.org/10.2174/0929867325666180104153848