摘要
背景:柑橘类生物活性化合物作为一种活性抗癌药物,一直是国内外研究的热点。然而,潜在的抗癌基因并没有。被充分强调了。 目的:研究柠檬烯对肝癌细胞HepG 2基因表达谱的影响。 方法:以浓度为50%的HepG 2细胞为研究对象,探讨柠檬烯抗癌作用的遗传机制。流式细胞术和流式细胞仪检测细胞凋亡情况。CAL荧光显微镜共聚焦荧光显微镜显示caspase-3激活和磷脂酰丝氨酸易位两种促凋亡作用.高吞吐量实时PC对16个与肿瘤发生相关的基因家族中的1023个相关基因进行了分析。 结果:与未处理细胞相比,流式细胞仪和荧光显微镜下柠檬烯细胞凋亡率分别提高到89.61%和48.2%。有一种重要的柠檬烯-15个不同基因家族HepG 2细胞的差异基因表达柠檬烯分别显著(>2log)上调和下调14个和59个基因。受影响者受影响最重的基因家族包括细胞凋亡诱导、信号转导、肿瘤基因增强、激酶表达改变、炎症、DNA损伤修复和ce。LL周期蛋白 结论:柠檬烯可作为一种有前途、价廉、有效的抗癌药物。广谱的柠檬烯抗癌活性对抗癌DRU有意义G发展。需要进一步的研究来证实目前的发现,并研究柠檬烯的抗癌潜力以及不同细胞系上的潜在机制。
关键词: 抗癌,癌症相关基因,黄酮类,HepG 2,高通量PCR,柠檬烯,抑癌剂。
图形摘要
Current Cancer Drug Targets
Title:A High-throughput Quantitative Expression Analysis of Cancer-related Genes in Human HepG2 Cells in Response to Limonene, a Potential Anticancer Agent
Volume: 18 Issue: 8
关键词: 抗癌,癌症相关基因,黄酮类,HepG 2,高通量PCR,柠檬烯,抑癌剂。
摘要: Background: Citrus bioactive compounds, as active anticancer agents, have been under focus by several studies worldwide. However, the underlying genes responsible for the anticancer potential have not been sufficiently highlighted.
Objectives: The current study investigated the gene expression profile of hepatocellular carcinoma, HepG2, cells after treatment with Limonene.
Methods: The concentration that killed 50% of HepG2 cells was used to elucidate the genetic mechanisms of limonene anticancer activity. The apoptotic induction was detected by flow cytometry and confocal fluorescence microscope. Two of the pro-apoptotic events, caspase-3 activation and phosphatidylserine translocation were manifested by confocal fluorescence microscopy. Highthroughput real-time PCR was used to profile 1023 cancer-related genes in 16 different gene families related to the cancer development.
Results: In comparison to untreated cells, limonene increased the percentage of apoptotic cells up to 89.61%, by flow cytometry, and 48.2% by fluorescence microscopy. There was a significant limonene- driven differential gene expression of HepG2 cells in 15 different gene families. Limonene was shown to significantly (>2log) up-regulate and down-regulate 14 and 59 genes, respectively. The affected gene families, from the most to the least affected, were apoptosis induction, signal transduction, cancer genes augmentation, alteration in kinases expression, inflammation, DNA damage repair, and cell cycle proteins.
Conclusion: The current study reveals that limonene could be a promising, cheap, and effective anticancer compound. The broad spectrum of limonene anticancer activity is interesting for anticancer drug development. Further research is needed to confirm the current findings and to examine the anticancer potential of limonene along with underlying mechanisms on different cell lines.
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A High-throughput Quantitative Expression Analysis of Cancer-related Genes in Human HepG2 Cells in Response to Limonene, a Potential Anticancer Agent, Current Cancer Drug Targets 2018; 18 (8) . https://dx.doi.org/10.2174/1568009617666171114144236
DOI https://dx.doi.org/10.2174/1568009617666171114144236 |
Print ISSN 1568-0096 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5576 |
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