摘要
背景:增生性玻璃体视网膜病变(PVR)是一个由分化的疾病,与多种细胞增殖迁移的地方。这些细胞形成纤维细胞膜导致视网膜脱离,视力丧失。PVR发生在原发性视网膜脱离(RD)患者中的8% - 10%,成为RD修复成功的主要障碍。视网膜色素上皮(RPE)细胞是主要的细胞组成的纤维细胞膜间。reproliferation和上皮间质转化(EMT)是PVR视网膜色素上皮细胞的主要病理改变。 方法:用不同浓度姜黄素处理RPE细胞24, 48、72小时。MTT法检测活细胞。RPE细胞的凋亡multicaspase V/7-AAD染色和流式细胞仪检测。PI染色定量分析细胞周期。实时荧光定量PCR检测mrna水平。蛋白印迹法检测蛋白水平。 结果:姜黄素对体外培养的RPE细胞增殖和EMT有明显的抑制作用。进一步研究表明姜黄素通过p53途径激活G2检查点诱导细胞周期阻滞。同时,我们发现姜黄素抑制RPE细胞中的Akt、MAPK和TGF-β通路,也可能影响增殖和EMT。我们的研究发现姜黄素是一种潜在的预防和治疗PVR的新药物。姜黄素激活G2检查点诱导细胞周期阻滞。 结论:本研究结果也为拓宽姜黄素在临床研究中的应用提供了思路。
关键词: 姜黄素,增生性玻璃体视网膜病变,视网膜色素上皮细胞、纤维细胞的细胞膜、细胞周期。
Current Molecular Medicine
Title:Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells Via Multiple Pathways
Volume: 17 Issue: 4
关键词: 姜黄素,增生性玻璃体视网膜病变,视网膜色素上皮细胞、纤维细胞的细胞膜、细胞周期。
摘要: Background: Proliferative vitreoretinopathy (PVR) is a disease caused by dedifferentiation, translocation and proliferation of several types of local cells. These cells form fibrocellular membranes resulting in detachment of retinal and vision loss. PVR occurs in 8%-10% of patients undergoing primary retinal detachment (RD) surgery and becomes a major obstacle for successful RD repair. Retinal pigment epithelial (RPE) cells are among the major cells which consist of fibrocellular membranes. Reproliferation and Epithelial-mesenchymal transition (EMT) are the primary pathological alteration of RPE cells in PVR.
Methods: RPE cells were treated with curcumin at different concentrations for 24, 48 and 72 hours. The viable cells were detected by MTT assay. The apoptosis of RPE was stained by Multicaspase/7-AAD and detected using flow cytometry. Cell cycle analysis was quantified by PI staining. The mRNA levels were detected by real-time PCR. The protein levels were detected by western blot.
Results: We found a compound curcumin significantly inhibited proliferation and EMT of RPE cells in vitro. Further study showed curcumin induced cell cycle arrest by activating G2 checkpoint through p53 pathway. Meanwhile, we found that curcumin suppressed the AKT, MAPK and TGF-β pathways in RPE cells which may also affect proliferation and EMT. Our research identified curcumin a potential novel agent for the PVR prevention and treatment. Curcumin induces cell cycle arrest by activating G2 checkpoint.
Conclusion: Our results in this study also provide the insights to broaden the application of curcumin in research and probably clinics.
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Cite this article as:
Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells Via Multiple Pathways, Current Molecular Medicine 2017; 17 (4) . https://dx.doi.org/10.2174/1566524017666171106115655
DOI https://dx.doi.org/10.2174/1566524017666171106115655 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
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