镜头原位再生的命运是由撕囊大小确定

Title:The Fate of In Situ Lens Regeneration is Determined by Capsulorhexis Size

Volume: 17 Issue: 4

关键词: 原位再生，晶状体，白内障连续环形撕囊，超声乳化，内源的干细胞。

摘要: Background and Objective: Lens regeneration is an optimal strategy for cataract patients to regain visual acuity with accommodation. We recently designed a novel, minimally invasive capsulorhexis surgical method for cataract removal that achieved functional lens regeneration in human infants. However, small anterior capsulorhexis requires advanced surgical expertise. To examine whether the quality of the regenerated lens can be maintained with enlarged anterior capsulorhexis, we investigated the shape and transparency of the regenerated lenses with different anterior capsulorhexis diameters (ACDs).

Methods: Thirty-six 4-week-old New Zealand albino rabbits were randomly divided into three groups which underwent lens extraction with different ACDs (Group A: 2.0±0.5 mm, Group B: 4.0±0.5 mm, Group C: 6.0±0.5 mm). The anterior capsule opening area (ACOA) was quantified, and the morphology, weight, and histological characteristics of the regenerated lenses were examined.

Results: Lens regeneration was observed in all three groups. In Group A, the regenerated lenses were relatively complete and transparent. In Groups B and C, the regenerated lenses were doughnut-shaped and opaque. The speed of lens regeneration in Group A was significantly faster than that in Groups B and C. The ACOA in Group A healed quickly and completely approximately 2 weeks after surgery. However, in Groups B and C, ACOA did not heal completely until 12 weeks after surgery. Histological examination showed that in Group A, most of the lens epithelial cells differentiated into well-organized lens fibers. However, in Groups B and C, the regenerated lens fibers were disorganized.

Conclusion: Capsulorhexis size is a critical determinant of integrity and transparency in lens regeneration.

Cite this article as:

The Fate of In Situ Lens Regeneration is Determined by Capsulorhexis Size, Current Molecular Medicine 2017; 17 (4) . https://dx.doi.org/10.2174/1566524017666171106110304